Trophic Transfer of Antibiotic Resistance Genes in a Soil Detritus Food Chain

2019 ◽  
Vol 53 (13) ◽  
pp. 7770-7781 ◽  
Author(s):  
Dong Zhu ◽  
Qian Xiang ◽  
Xiao-Ru Yang ◽  
Xin Ke ◽  
Patrick O’Connor ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Food Chain ◽  
Antibiotic Resistance Genes ◽  
Trophic Transfer

scholarly journals Investigation of Some Antibiotic Resistance Genes of Indigenous Lactobacilli Isolated From Traditional Yogurt In Zanjan Province of Iran

2021 ◽  
Author(s):  
Bahare Moghimi ◽  
Maryam Ghobadi Dana ◽  
Reza Shapouri
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Food Chain ◽  
Starter Culture ◽  
Antibiotic Resistance Genes ◽  
Molecular Methods ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Fermented Foods ◽  
Antibiotic Resistant

Abstract Purpose: Given the increasing use of antibiotics on humans and livestock for treatment or as a growth stimulant, antibiotic resistance has become a general concern. The food chain and specially fermented foods could be a source of antibiotic-resistant bacteria and resistance genes. Lactic Acid Bacteria (LAB) and Lactobacilli are considered safe to use as starter culture or probiotic strains. Recently, however, antibiotic-resistant genes isolated from LABs showed the necessity of setting international regulations to reduce the risk of antibiotic resistance genes transmission via the food chain. The current study aimed to investigate the antibiotic resistance of Lactobacilli isolated from traditional yogurt samples from Zanjan province in Iran.Methods: Lactobacilli characterization and identification were carried out through biochemical and molecular methods. The disk diffusion method was applied to determine phenotype resistance using 13 antibiotic disks resistance genes presence were investigated in the isolates to determine transferability risk, respectively.Results: Based on biochemical and molecular methods, 24 isolates have been identified as Lactobacilli with multiple antibiotic-resistant phenotypes. Vancomycin resistance was a typical phenotype and genotype among isolates. On investigated Lactobacilli chromosome, Tetracycline resistance genes Chloramphenicol (cat), beta-lactam, aminoglycosides (aph (3’)-III), and aadA resistance genes have been detected. While the examined resistance genes have not been detected on the plasmids, they were all on the bacterial chromosome.Conclusion: The results showed that the investigated isolates did not carry the resistance genes on their plasmids. It, therefore, would be a good point since they probably do not transfer resistance genes to other bacteria, and they would be proper candidates to do more investigation for introducing new safe starter culture or probiotic strain to food industries.

Antibiotic resistance genes back in the food chain

2001 ◽  
Vol 17 (7) ◽  
pp. 381
Author(s):  
P Ligoxygakis
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Food Chain ◽  
Antibiotic Resistance Genes

Chironomidae larvae: A neglected enricher of antibiotic resistance genes in the food chain of freshwater environments

2021 ◽  
pp. 117486
Author(s):  
Chengshi Ding ◽  
Jing Ma ◽  
Wanxiang Jiang ◽  
Hanyu Zhao ◽  
Mengmeng Shi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Food Chain ◽  
Antibiotic Resistance Genes ◽  
Chironomidae Larvae ◽  
Freshwater Environments

Quintuplex PCR to Detect Antibiotic Resistance Genes in Streptococcus pneumoniae

2016 ◽  
Vol 1 (2) ◽  
pp. 22 ◽  
Author(s):  
Navindra Kumari Palanisamy ◽  
Parasakthi Navaratnam ◽  
Shamala Devi Sekaran
Keyword(s):  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Bacterial Pathogen ◽  
Antibiotic Resistance Genes ◽  
Pcr Amplification ◽  
Penicillin Resistance ◽  
Penicillin Binding Proteins ◽  
Efflux Mechanism ◽  
Mic Value

Introduction: Streptococcus pneumoniae is an important bacterial pathogen, causing respiratory infection. Penicillin resistance in S. pneumoniae is associated with alterations in the penicillin binding proteins, while resistance to macrolides is conferred either by the modification of the ribosomal target site or efflux mechanism. This study aimed to characterize S. pneumoniae and its antibiotic resistance genes using 2 sets of multiplex PCRs. Methods: A quintuplex and triplex PCR was used to characterize the pbp1A, ermB, gyrA, ply, and the mefE genes. Fifty-eight penicillin sensitive strains (PSSP), 36 penicillin intermediate strains (PISP) and 26 penicillin resistance strains (PRSP) were used. Results: Alteration in pbp1A was only observed in PISP and PRSP strains, while PCR amplification of the ermB or mefE was observed only in strains with reduced susceptibility to erythromycin. The assay was found to be sensitive as simulated blood cultures showed the lowest level of detection to be 10cfu. Conclusions: As predicted, the assay was able to differentiate penicillin susceptible from the non-susceptible strains based on the detection of the pbp1A gene, which correlated with the MIC value of the strains.

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