Highly Efficient Production of N-Acetyl-glucosamine in Escherichia coli by Appropriate Catabolic Division of Labor in the Utilization of Mixed Glycerol/Glucose Carbon Sources

2021 ◽  
Author(s):  
Qian Ma ◽  
Quanwei Sun ◽  
Miao Tan ◽  
Li Xia ◽  
Ying Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Division Of Labor ◽  
Carbon Sources ◽  
Efficient Production ◽  
Highly Efficient ◽  
Glucose Carbon

Highly Efficient Production of l-Histidine from Glucose by Metabolically Engineered Escherichia coli

2020 ◽  
Vol 9 (7) ◽  
pp. 1813-1822
Author(s):  
Heyun Wu ◽  
Daoguang Tian ◽  
Xiaoguang Fan ◽  
Weiming Fan ◽  
Yue Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Efficient Production ◽  
Highly Efficient ◽  
Engineered Escherichia Coli ◽  
Metabolically Engineered Escherichia Coli

scholarly journals Metabolic engineering Escherichia coli for efficient production of icariside D2

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Xue Liu ◽  
Lingling Li ◽  
Jincong Liu ◽  
Jianjun Qiao ◽  
Guang-Rong Zhao
Keyword(s):  
Escherichia Coli ◽  
De Novo ◽  
Carbon Sources ◽  
Gene Expression Pattern ◽  
Fine Tuning ◽  
Cell Factory ◽  
Efficient Production ◽  
Uridine Diphosphate ◽  
Microbial Cell Factory ◽  
E Coli

Abstract Background Icariside D2 is a plant-derived natural glycoside with pharmacological activities of inhibiting angiotensin-converting enzyme and killing leukemia cancer cells. Production of icariside D2 by plant extraction and chemical synthesis is inefficient and environmentally unfriendly. Microbial cell factory offers an attractive route for economical production of icariside D2 from renewable and sustainable bioresources. Results We metabolically constructed the biosynthetic pathway of icariside D2 in engineered Escherichia coli. We screened the uridine diphosphate glycosyltransferases (UGTs) and obtained an active RrUGT3 that regio-specifically glycosylated tyrosol at phenolic position to exclusively synthesize icariside D2. We put heterologous genes in E. coli cell for the de novo biosynthesis of icariside D2. By fine-tuning promoter and copy number as well as balancing gene expression pattern to decrease metabolic burden, the BMD10 monoculture was constructed. Parallelly, for balancing pathway strength, we established the BMT23–BMD12 coculture by distributing the icariside D2 biosynthetic genes to two E. coli strains BMT23 and BMD12, responsible for biosynthesis of tyrosol from preferential xylose and icariside D2 from glucose, respectively. Under the optimal conditions in fed-batch shake-flask fermentation, the BMD10 monoculture produced 3.80 g/L of icariside D2 using glucose as sole carbon source, and the BMT23–BMD12 coculture produced 2.92 g/L of icariside D2 using glucose–xylose mixture. Conclusions We for the first time reported the engineered E. coli for the de novo efficient production of icariside D2 with gram titer. It would be potent and sustainable approach for microbial production of icariside D2 from renewable carbon sources. E. coli–E. coli coculture approach is not limited to glycoside production, but could also be applied to other bioproducts.

Highly efficient production of L-Homoserine in Escherichia coli by engineering a redox balance route

2021 ◽  
Author(s):  
Qingxuan Mu ◽  
Shasha Zhang ◽  
Xianjun Mao ◽  
Yong Tao ◽  
Bo Yu
Keyword(s):  
Escherichia Coli ◽  
Redox Balance ◽  
Efficient Production ◽  
Highly Efficient
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