Time-course RNASeq of Camponotus floridanus forager and nurse ant brains indicate links between plasticity in the biological clock and behavioral division of labor

Background Circadian clocks allow organisms to anticipate daily fluctuations in their environment by driving rhythms in physiology and behavior. Inter-organismal differences in daily rhythms, called chronotypes, exist and can shift with age. In ants, age, caste-related behavior and chronotype appear to be linked. Brood-tending nurse ants are usually younger individuals and show “around-the-clock” activity. With age or in the absence of brood, nurses transition into foraging ants that show daily rhythms in activity. Ants can adaptively shift between these behavioral castes and caste-associated chronotypes depending on social context. We investigated how changes in daily gene expression could be contributing to such behavioral plasticity in Camponotus floridanus carpenter ants by combining time-course behavioral assays and RNA-Sequencing of forager and nurse brains. Results We found that nurse brains have three times fewer 24 h oscillating genes than foragers. However, several hundred genes that oscillated every 24 h in forager brains showed robust 8 h oscillations in nurses, including the core clock genes Period and Shaggy. These differentially rhythmic genes consisted of several components of the circadian entrainment and output pathway, including genes said to be involved in regulating insect locomotory behavior. We also found that Vitellogenin, known to regulate division of labor in social insects, showed robust 24 h oscillations in nurse brains but not in foragers. Finally, we found significant overlap between genes differentially expressed between the two ant castes and genes that show ultradian rhythms in daily expression. Conclusion This study provides a first look at the chronobiological differences in gene expression between forager and nurse ant brains. This endeavor allowed us to identify a putative molecular mechanism underlying plastic timekeeping: several components of the ant circadian clock and its output can seemingly oscillate at different harmonics of the circadian rhythm. We propose that such chronobiological plasticity has evolved to allow for distinct regulatory networks that underlie behavioral castes, while supporting swift caste transitions in response to colony demands. Behavioral division of labor is common among social insects. The links between chronobiological and behavioral plasticity that we found in C. floridanus, thus, likely represent a more general phenomenon that warrants further investigation.

Staging the Social Drama of Work: Ethnography of a Theater Company as a Means of Analyzing Theater Activity

This paper shows how conducting the ethnographic study of a theater hall and company can help define theater activity. Once the aesthetic of the social organization is set apart from the proper division of labor, theater appears as a collective activity which requires the cooperation of eight groups playing different social roles. The cooperation modes rest on a meshing of direct or indirect services for the actors who carry out the core task of performing. This specific organization of work around a central group is what makes the activity artistic. Simultaneously, the service relation offers the possibility for some categories to bring their relationship with actors closer to a state of symmetry and sometimes reverse asymmetry. As a status enhancing opportunity, service relationship for actors also directly or indirectly provide the grounds for participant commitment and thus guarantee long-lasting operation for the theatrical organization.

THE SUPERVISORY PERFORMANCE OF BAWASLU HULU SUNGAI UTARA IN THE 2019 ELECTION

The Hulu Sungai Utara Regency Election Supervisory Body is an Election organizing body tasked with overseeing the implementation of Elections, the problem is the improper installation and / or distribution of Campaign Props (Campaign), Campaign Materials (BK), Unclear division of labor. The purpose of this study was to determine the Performance of the Election Oversight Body in the Supervision of Election of the House of Representatives, Members of the Regional Representative Council, President and Vice President in 2019 at the Election Supervisory Body (Bawaslu) of North Hulu Sungai Regency and the factors that influence it. This research uses a qualitative approach with descriptive-qualitative type. Data collection techniques used were interviews, observation and documentation. Source of data taken through a purposive withdrawal of 15 people. After the data is collected, it is then analyzed using techniques including data reduction, data presentation, and data verification or drawing conclusions. Test the credibility of the data in this study is the extension of observation, increase perseverance, triangulation, negative case analysis, and hold a member check. The results of the research on the performance of the Election Oversight Body in the Supervision of the Election of the House of Representatives, Members of the Regional Representative Council, the President and Vice President in 2019 on the Election Supervisory Body (Bawaslu) of North Hulu Sungai Regency have not been good this can be seen from several indicators.

Gender and Mindscapes of Patriarchy in Poverty Reduction

Is poverty gendered? Feminist theorists suggest that the experience of poverty by any woman is shaped not only by her gender but by ideologies and other systems of social stratification such as race, ethnicity, and class – and that these dimensions are not simply an additional facet of a woman's identity but do affect her gendered experience as well. The narratives examined in this chapter consist of symbolic patriarchy, inheritance laws, gender socialization, domestic division of labor, and certain accounts pertaining to widows and the subordination of women. A scan of the African cultural landscape revealed a profile of rural poverty that is unproportionate, unequal, and sometimes unfairly affects women more than men. Patriarchal and discriminatory practices are stubbornly unyielding in some rural areas and has inflicted a blow against women the most.

Mulheres e futebol: a cobertura sobre o Brasileirão Feminino no site globoesporte.com

O presente trabalho tem como proposta identificar e categorizar as notícias relativas ao Campeonato Brasileiro Feminino 2021 no site globoesporte.com. Aborda o conceito de gênero como um elemento diretamente ligado as relações sociais. Reflete sobre a divisão sexual do trabalho e os papéis sociais de gênero. Comenta sobre a presença da mulher no futebol. É uma pesquisa bibliográfica e quali-quantitativa. Apresenta a estrutura do Campeonato Brasileiro Feminino 2021. Aponta a desigualdade de gênero como a causa das discrepâncias entre o futebol masculino e feminino. Conclui que o site em questão realizou ampla cobertura do campeonato, pois noticiou todas as fases do campeonato.Palavras-chave: Futebol feminino. Campeonato Brasileiro Feminino. Globo Esporte. Women and football: the coverage of the Brazilian Female Championship at globoesporte.comABSTRACTThis paper aims to identify and categorize the news related to the Brazilian Female Championship 2021 on the globoesporte.com website. It approaches the concept of gender as an element directly linked to social relations. It reflects on the sexual division of labor and social gender roles. It comments on the presence of women in soccer. It is a bibliographic and qualitative-quantitative research. Presents the structure of the 2021 Brazilian Women's Championship. Points to gender inequality as the cause of the differences between male and female soccer. It concludes that the site in question carried out extensive coverage of the championship, seeing that it reported all phases of the championship.Keywords: Women’s footbal. Brazilian Female Championship. Globo Esporte.

Strain Improvement and Strain Maintenance Revisited. The Use of Actinoplanes teichomyceticus ATCC 31121 Protoplasts in the Identification of Candidates for Enhanced Teicoplanin Production

Multicellular cooperation in actinomycetes is a division of labor-based beneficial trait where phenotypically specialized clonal subpopulations, or genetically distinct lineages, perform complementary tasks. The division of labor improves the access to nutrients and optimizes reproductive and vegetative tasks while reducing the costly production of secondary metabolites and/or of secreted enzymes. In this study, we took advantage of the possibility to isolate genetically distinct lineages deriving from the division of labor, for the isolation of heterogeneous teicoplanin producer phenotypes from Actinoplanes teichomyceticus ATCC 31121. In order to efficiently separate phenotypes and associated genomes, we produced and regenerated protoplasts. This approach turned out to be a rapid and effective strain improvement method, as it allowed the identification of those phenotypes in the population that produced higher teicoplanin amounts. Interestingly, a heterogeneous teicoplanin complex productivity pattern was also identified among the clones. This study suggests that strain improvement and strain maintenance should be integrated with the use of protoplasts as a strategy to unravel the hidden industrial potential of vegetative mycelium.