Characterization of CsTSI in the Biosynthesis of Theanine in Tea Plants (Camellia sinensis)

2022 ◽  
Author(s):  
Guangbiao She ◽  
Shuwei Yu ◽  
Zhenguo Li ◽  
Anqi Peng ◽  
Penghui Li ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Tea Plants

scholarly journals Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Peixian Bai ◽  
Liyuan Wang ◽  
Kang Wei ◽  
Li Ruan ◽  
Liyun Wu ◽  
...  
Keyword(s):  
Gene Duplication ◽  
Camellia Sinensis ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Protein Sequences ◽  
Biochemical Properties ◽  
High Similarity ◽  
New Gene ◽  
Tea Plants

Abstract Background Alanine decarboxylase (AlaDC), specifically present in tea plants, is crucial for theanine biosynthesis. Serine decarboxylase (SDC), found in many plants, is a protein most closely related to AlaDC. To investigate whether the new gene AlaDC originate from gene SDC and to determine the biochemical properties of the two proteins from Camellia sinensis, the sequences of CsAlaDC and CsSDC were analyzed and the two proteins were over-expressed, purified, and characterized. Results The results showed that exon-intron structures of AlaDC and SDC were quite similar and the protein sequences, encoded by the two genes, shared a high similarity of 85.1%, revealing that new gene AlaDC originated from SDC by gene duplication. CsAlaDC and CsSDC catalyzed the decarboxylation of alanine and serine, respectively. CsAlaDC and CsSDC exhibited the optimal activities at 45 °C (pH 8.0) and 40 °C (pH 7.0), respectively. CsAlaDC was stable under 30 °C (pH 7.0) and CsSDC was stable under 40 °C (pH 6.0–8.0). The activities of the two enzymes were greatly enhanced by the presence of pyridoxal-5′-phosphate. The specific activity of CsSDC (30,488 IU/mg) was 8.8-fold higher than that of CsAlaDC (3467 IU/mg). Conclusions Comparing to CsAlaDC, its ancestral enzyme CsSDC exhibited a higher specific activity and a better thermal and pH stability, indicating that CsSDC acquired the optimized function after a longer evolutionary period. The biochemical properties of CsAlaDC might offer reference for theanine industrial production.

Characterization of fluoride uptake by roots of tea plants (Camellia sinensis (L.) O. Kuntze)

2012 ◽  
Vol 366 (1-2) ◽  
pp. 659-669 ◽  
Author(s):  
Lei Zhang ◽  
Qiong Li ◽  
Lifeng Ma ◽  
Jianyun Ruan
Keyword(s):  
Camellia Sinensis ◽  
Fluoride Uptake ◽  
Tea Plants

scholarly journals Characterization of the R2R3-MYB Transcription Factor CsMYB113 Regulates Anthocyanin Biosynthesis in Tea Plants (Camellia Sinensis)

2021 ◽  
Author(s):  
Liuyuan Shui ◽  
Meilin Yan ◽  
Hui Li ◽  
Pu Wang ◽  
Hua Zhao ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Anthocyanin Biosynthesis ◽  
Tea Plant ◽  
Myb Transcription Factor ◽  
Germplasm Resources ◽  
Characteristic Variety ◽  
History Of ◽  
Tea Plants ◽  
R2r3 Myb

Abstract Tea plant(Camellia sinensis) has very long history of cultivation and abundant germplasm resources in China. Purple bud is a characteristic variety, which has attracted the attention of breeding researchers because it accumulated a large number of anthocyanins naturally. In many species, R2R3-MYBtranscription factors (TFs)wereprovedto be involved in the regulation of anthocyanin biosynthesis.Research on anthocyanin metabolism has been relatively clear in some species, but that needs to be further elucidated in tea plants. In this research, anR2R3-MYB transcriptionfactor CsMYB113 relate to the anthocyanin accumulation regulation was identified from tea plants. Spatial and temporal expressionanalysis revealed differential expression of CsMYB113among different tissues and organs, with highest expression occurringin the roots.Subcellular localization assays showed that CsMYB113 localizedin the nucleus.Ectopic expression of CsMYB113increased pigmentation and anthocyanin contentsby the up-regulationof theexpression levelsof genes in anthocyanin biosynthesis pathwayamongdifferent tissues of Arabidopsis.Moreover, transient overexpressionof 35S::CsMYB113in tea plant increased the anthocyanin contents in the leaves.Our results indicated that CsMYB113 play important role in the anthocyaninbiosynthesis regulation in tea plants. It will also provide useful candidate gene for the modification of anthocyanin metabolism by genetic engineeringin plants.

Molecular Basis of the Distinct Metabolic Features in Shoot Tips and Roots of Tea Plants (Camellia sinensis): Characterization of MYB Regulator for Root Theanine Synthesis

2021 ◽  
Vol 69 (11) ◽  
pp. 3415-3429
Author(s):  
Yanrui Zhang ◽  
Penghui Li ◽  
Guangbiao She ◽  
Yujie Xu ◽  
Anqi Peng ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Molecular Basis ◽  
Shoot Tips ◽  
Tea Plants

GABA shunt contribution to flavonoid biosynthesis and metabolism in tea plants (Camellia sinensis)

2021 ◽  
Author(s):  
Jieren Liao ◽  
Qiang Shen ◽  
Ruiyang Li ◽  
Yu Cao ◽  
Yue Li ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Flavonoid Biosynthesis ◽  
Gaba Shunt ◽  
Tea Plants
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