Molecular Characterization of WRKY Transcription Factors That Act as Negative Regulators of O-Methylated Catechin Biosynthesis in Tea Plants (Camellia sinensis L.)

2018 ◽  
Vol 66 (43) ◽  
pp. 11234-11243 ◽  
Author(s):  
Yong Luo ◽  
Shuangshuang Yu ◽  
Juan Li ◽  
Qin Li ◽  
Kunbo Wang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Molecular Characterization ◽  
Camellia Sinensis ◽  
Wrky Transcription Factors ◽  
Negative Regulators ◽  
Tea Plants

scholarly journals Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Peixian Bai ◽  
Liyuan Wang ◽  
Kang Wei ◽  
Li Ruan ◽  
Liyun Wu ◽  
...  
Keyword(s):  
Gene Duplication ◽  
Camellia Sinensis ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Protein Sequences ◽  
Biochemical Properties ◽  
High Similarity ◽  
New Gene ◽  
Tea Plants

Abstract Background Alanine decarboxylase (AlaDC), specifically present in tea plants, is crucial for theanine biosynthesis. Serine decarboxylase (SDC), found in many plants, is a protein most closely related to AlaDC. To investigate whether the new gene AlaDC originate from gene SDC and to determine the biochemical properties of the two proteins from Camellia sinensis, the sequences of CsAlaDC and CsSDC were analyzed and the two proteins were over-expressed, purified, and characterized. Results The results showed that exon-intron structures of AlaDC and SDC were quite similar and the protein sequences, encoded by the two genes, shared a high similarity of 85.1%, revealing that new gene AlaDC originated from SDC by gene duplication. CsAlaDC and CsSDC catalyzed the decarboxylation of alanine and serine, respectively. CsAlaDC and CsSDC exhibited the optimal activities at 45 °C (pH 8.0) and 40 °C (pH 7.0), respectively. CsAlaDC was stable under 30 °C (pH 7.0) and CsSDC was stable under 40 °C (pH 6.0–8.0). The activities of the two enzymes were greatly enhanced by the presence of pyridoxal-5′-phosphate. The specific activity of CsSDC (30,488 IU/mg) was 8.8-fold higher than that of CsAlaDC (3467 IU/mg). Conclusions Comparing to CsAlaDC, its ancestral enzyme CsSDC exhibited a higher specific activity and a better thermal and pH stability, indicating that CsSDC acquired the optimized function after a longer evolutionary period. The biochemical properties of CsAlaDC might offer reference for theanine industrial production.

scholarly journals Molecular Characterization of HOXA2 and HOXA3 Binding Properties

2021 ◽  
Vol 9 (4) ◽  
pp. 55
Author(s):  
Joshua Mallen ◽  
Manisha Kalsan ◽  
Peyman Zarrineh ◽  
Laure Bridoux ◽  
Shandar Ahmad ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Molecular Characterization ◽  
Sequence Motif ◽  
Body Parts ◽  
Binding Affinities ◽  
Binding Properties ◽  
Functional Consequences

The highly conserved HOX homeodomain (HD) transcription factors (TFs) establish the identity of different body parts along the antero–posterior axis of bilaterian animals. Segment diversification and the morphogenesis of different structures is achieved by generating precise patterns of HOX expression along the antero–posterior axis and by the ability of different HOX TFs to instruct unique and specific transcriptional programs. However, HOX binding properties in vitro, characterised by the recognition of similar AT-rich binding sequences, do not account for the ability of different HOX to instruct segment-specific transcriptional programs. To address this problem, we previously compared HOXA2 and HOXA3 binding in vivo. Here, we explore if sequence motif enrichments observed in vivo are explained by binding affinities in vitro. Unexpectedly, we found that the highest enriched motif in HOXA2 peaks was not recognised by HOXA2 in vitro, highlighting the importance of investigating HOX binding in its physiological context. We also report the ability of HOXA2 and HOXA3 to heterodimerise, which may have functional consequences for the HOX patterning function in vivo.

Analysis of WRKY transcription factors and characterization of two Botrytis cinerea-responsive LrWRKY genes from Lilium regale

2018 ◽  
Vol 127 ◽  
pp. 525-536 ◽  
Author(s):  
Qi Cui ◽  
Xiao Yan ◽  
Xue Gao ◽  
Dong-mei Zhang ◽  
Heng-bin He ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Botrytis Cinerea ◽  
Wrky Transcription Factors ◽  
Lilium Regale

scholarly journals Characterization of Citrus WRKY transcription factors and their responses to phytohormones and abiotic stresses

2018 ◽  
Vol 62 (1) ◽  
pp. 33-44 ◽  
Author(s):  
V. Vives-Peris ◽  
D. Marmaneu ◽  
A. Gomez-Cadenas ◽  
R. M. Perez-Clemente
Keyword(s):  
Transcription Factors ◽  
Abiotic Stresses ◽  
Wrky Transcription Factors

Expression and Molecular Characterization of ZmMYB-IF35 and Related R2R3-MYB Transcription Factors

2007 ◽  
Vol 37 (2) ◽  
pp. 155-164 ◽  
Author(s):  
George F. Heine ◽  
Vinod Malik ◽  
Anusha P. Dias ◽  
Erich Grotewold
Keyword(s):  
Transcription Factors ◽  
Molecular Characterization ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

Molecular characterization of cold-responsive basic helix-loop-helix transcription factors MabHLHs that interact with MaICE1 in banana fruit

Planta ◽  
2013 ◽  
Vol 238 (5) ◽  
pp. 937-953 ◽  
Author(s):  
Huan-huan Peng ◽  
Wei Shan ◽  
Jian-fei Kuang ◽  
Wang-jin Lu ◽  
Jian-ye Chen
Keyword(s):  
Transcription Factors ◽  
Molecular Characterization ◽  
Banana Fruit ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

Identification and characterization of hemp WRKY transcription factors in response to abiotic stresses

2016 ◽  
Vol 60 (3) ◽  
pp. 489-495 ◽  
Author(s):  
P.-F. Xin ◽  
C.-S. Gao ◽  
C.-H. Cheng ◽  
Q. Tang ◽  
Z.-X. Dong ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Abiotic Stresses ◽  
Wrky Transcription Factors ◽  
Identification And Characterization
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