Reformulating the Hydrolytic Enzyme Cocktail of Trichoderma reesei by Combining XYR1 Overexpression and Elimination of Four Major Cellulases to Improve Saccharification of Corn Fiber

2021 ◽  
Author(s):  
Weixin Zhang ◽  
Junqi Guo ◽  
Xiaoxiao Wu ◽  
Yajing Ren ◽  
Chunyan Li ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Hydrolytic Enzyme ◽  
Corn Fiber ◽  
Enzyme Cocktail

Profile of Enzyme Production by Trichoderma reesei Grown on Corn Fiber Fractions

2005 ◽  
Vol 121 (1-3) ◽  
pp. 0321-0334 ◽  
Author(s):  
Xin-Liang Li ◽  
Bruce S. Dien ◽  
Michael A. Cotta ◽  
Y. Victor Wu ◽  
Badal C. Saha
Keyword(s):  
Trichoderma Reesei ◽  
Enzyme Production ◽  
Corn Fiber ◽  
Fiber Fractions

Profile of Enzyme Production by Trichoderma reesei Grown on Corn Fiber Fractions

2009 ◽  
pp. 321-334 ◽  
Author(s):  
Xin-Liang Li ◽  
Bruce S. Dien ◽  
Michael A. Cotta ◽  
Y. Victor Wu ◽  
Badal C. Saha
Keyword(s):  
Trichoderma Reesei ◽  
Enzyme Production ◽  
Corn Fiber ◽  
Fiber Fractions

scholarly journals A novel Swollenin from Talaromyces leycettanus JCM12802 enhances cellulase hydrolysis towards various substrates

2020 ◽  
Author(s):  
Honghai Zhang ◽  
Yuan Wang ◽  
Roman Brunecky ◽  
Bin Yao ◽  
Xiangming Xie ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Functional Diversity ◽  
Catalytic Mechanism ◽  
Specific Activity ◽  
Synergistic Effects ◽  
Hydrolytic Enzyme ◽  
Industrial Applications ◽  
Maximum Activity ◽  
Carbohydrate Binding ◽  
Cell Wall Polysaccharides

Abstract Background: Swollenins exist within some fungal species such as Trichoderma reesei and Aspergillus niger, and are candidate accessory enzymes for the biodegradation of cellulosic substrates. Swollenins theorized role in biological processes is as promoters in the rearrangement of non-covalent interaction networks formed by cell wall polysaccharides. Such network rearrangements though to be responsible for expanding accessibility for hydrolytic enzyme degradation processes. We hereby report in detail the characterization of a recombinant swollenin and its disruptive activity on cellulosic substrates and its synergistic effects while interacting with cellulases.Results: We have expressed a novel swollenin gene Tlswo consists of an open reading frame encoding 503 amino acids to be identified from Talaromyces leycettanus JCM12802 and successfully expressed it in both Trichoderma reesei and Pichia pastoris. Similar to other fungal swollenins, TlSWO is composed of a N-terminal family 1 carbohydrate binding module (CBM1) followed by a Ser/Thr rich linker connected to an expansin-like domain, followed by a family of 45 endoglucanase-like domains and a group-2 grass pollen allergen domain. From our experimental results, TlSWO demonstrated disruptive activity on Avicel and displayed a synergistic effect with cellobiohydrolases, enhancing its hydrolytic performance up to 132%. We also explored the activity of TlSWO on several model substrates as well as pretreated biomass. Our results indicate that TlSWO is capable of releasing reducing sugars from lichenan, barley β-glucan, carboxymethyl cellulose sodium (CMC-Na) and laminarin. The specific activity of TlSWO towards the substates mentioned above is 9.0 ± 0.100 U/mg, 8.9 ± 0.100U/mg, 2.3 ± 0.002 U/mg and 0.79 ± 0.002 U/mg, respectively. Moreover, further experimentation confirms the fungal swollenin TlSWO exhibits maximum activity at pH 4.0 and 50 ℃.Conclusion: This study reports on a novel swollenin protein capable of enhancing biomass conversion. Experimental data reveals the functional diversity of this swollenin with considerable activity on various substrates. Although the exact catalytic mechanism of swollenin’s remains unknown, the functional diversity of TlSWO broadens its applicability under experimental settings, and suggests it may be a promising candidate for future industrial applications.

Cellulase production and morphology of Trichoderma reesei in different experimental conditions

2018 ◽  
Vol 63 (2) ◽  
pp. 115-129
Author(s):  
Rahela Carpa ◽  
◽  
Alin Cândea ◽  
Alexei Remizovschi ◽  
Lucian Barbu-Tudoran ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production ◽  
Experimental Conditions

Identification and characterization of a novel gene, TrCCD1, and its possible function in hyphal growth and conidiospore development of Trichoderma reesei

2009 ◽  
Vol 46 (3) ◽  
pp. 255-263 ◽  
Author(s):  
Yao Hua Zhong ◽  
Tian Hong Wang ◽  
Xiao Li Wang ◽  
Guang Tao Zhang ◽  
Hai Na Yu
Keyword(s):  
Trichoderma Reesei ◽  
Hyphal Growth ◽  
Novel Gene ◽  
Identification And Characterization

Statistical study of mechanical properties for corn fiber with reinforced of polypropylene fiber matrix composite

2021 ◽  
Author(s):  
Sameer A. Agrawal ◽  
Ashish M. Umbarkar ◽  
Nitin P. Sherie ◽  
Ashish M. Dharme ◽  
Dharmesh Dhabliya
Keyword(s):  
Mechanical Properties ◽  
Matrix Composite ◽  
Statistical Study ◽  
Polypropylene Fiber ◽  
Corn Fiber ◽  
Fiber Matrix
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