Proteomic Analysis of G2/M Arrest Triggered by Natural Borneol/Curcumin in HepG2 Cells, the Importance of the Reactive Oxygen Species-p53 Pathway

2015 ◽  
Vol 63 (28) ◽  
pp. 6440-6449 ◽  
Author(s):  
Jianping Chen ◽  
Lin Li ◽  
Jianyu Su ◽  
Bing Li ◽  
Xia Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Proteomic Analysis ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
P53 Pathway

scholarly journals Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-8
Author(s):  
Xiao-Tian Zhang ◽  
Chun-Jiang Yu ◽  
Jian-Wei Liu ◽  
Yan-Ping Zhang ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Palmitic Acid ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Glucose Consumption ◽  
Control Group ◽  
High Dose ◽  
Oxygen Species ◽  
Insulin Resistant

We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P< 0.001); however, QJJ restored HepG2 cell survival (P< 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (allP< 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (allP< 0.05). Furthermore, high-dose QJJ reduced both TNF-αand IL-6 levels as compared to the IR group (allP< 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.

Reactive oxygen species-dependent JNK downregulated olaquindox-induced autophagy in HepG2 cells

2014 ◽  
Vol 35 (7) ◽  
pp. 709-716 ◽  
Author(s):  
Dongxu Zhao ◽  
Congcong Wang ◽  
Shusheng Tang ◽  
Chaoming Zhang ◽  
Shen Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

Neferine induces reactive oxygen species mediated intrinsic pathway of apoptosis in HepG2 cells

2013 ◽  
Vol 136 (2) ◽  
pp. 659-667 ◽  
Author(s):  
Paramasivan Poornima ◽  
Robin Sheeba Quency ◽  
Viswandha Vijaya Padma
Keyword(s):  
Reactive Oxygen Species ◽  
Hepg2 Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intrinsic Pathway
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