Catalytic, Computational, and Evolutionary Analysis of thed-Lactate Dehydrogenases Responsible ford-Lactic Acid Production in Lactic Acid Bacteria

2018 ◽  
Vol 66 (31) ◽  
pp. 8371-8381 ◽  
Author(s):  
Baolei Jia ◽  
Zhong Ji Pu ◽  
Ke Tang ◽  
Xiaomeng Jia ◽  
Kyung Hyun Kim ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Evolutionary Analysis ◽  
Lactate Dehydrogenases

Incidence of Listeria monocytogenes in Silage and Its Subsequent Control by Specific and Nonspecific Antagonism

1990 ◽  
Vol 53 (8) ◽  
pp. 642-647 ◽  
Author(s):  
CURTT M. PERRY ◽  
CATHERINE W. DONNELLY
Keyword(s):  
Lactic Acid ◽  
Listeria Monocytogenes ◽  
Lactic Acid Bacteria ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Dairy Farms ◽  
Listeria Innocua ◽  
Listeria Species

Silage samples representing approximately 10% of Vermont's dairy farms were tested for the presence of Listeria species. Listeria innocua was isolated from 15.3% of the silage samples, while Listeria monocytogenes was isolated from 2.9% of the examined samples. As silage pH increased, the incidence of Listeria increased concomitantly. Seventy-eight mesophilic lactic acid bacteria, indigenous to silage, were screened for specific and nonspecific antagonism against four L. monocytogenes indicator strains. Most of the silage isolates demonstrated nonspecific inhibition via lactic acid production against the L. monocytogenes indicator strains. None of the indigenous silage isolates tested in this survey demonstrated specific antagonism via production of bacteriocinogenic compounds.

scholarly journals Improvement of Lactic Acid Production in Saccharomyces cerevisiae by Cell Sorting for High Intracellular pH

2006 ◽  
Vol 72 (8) ◽  
pp. 5492-5499 ◽  
Author(s):  
Minoska Valli ◽  
Michael Sauer ◽  
Paola Branduardi ◽  
Nicole Borth ◽  
Danilo Porro ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Intracellular Ph ◽  
Cell Sorting ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Low Ph ◽  
The Mean ◽  
High Level ◽  
Lactate Dehydrogenases

ABSTRACT Yeast strains expressing heterologous l-lactate dehydrogenases can produce lactic acid. Although these microorganisms are tolerant of acidic environments, it is known that at low pH, lactic acid exerts a high level of stress on the cells. In the present study we analyzed intracellular pH (pHi) and viability by staining with cSNARF-4F and ethidium bromide, respectively, of two lactic-acid-producing strains of Saccharomyces cerevisiae, CEN.PK m850 and CEN.PK RWB876. The results showed that the strain producing more lactic acid, CEN.PK m850, has a higher pHi. During batch culture, we observed in both strains a reduction of the mean pHi and the appearance of a subpopulation of cells with low pHi. Simultaneous analysis of pHi and viability proved that the cells with low pHi were dead. Based on the observation that the better lactic-acid-producing strain had a higher pHi and that the cells with low pHi were dead, we hypothesized that we might find better lactic acid producers by screening for cells within the highest pHi range. The screening was performed on UV-mutagenized populations through three consecutive rounds of cell sorting in which only the viable cells within the highest pHi range were selected. The results showed that lactic acid production was significantly improved in the majority of the mutants obtained compared to the parental strains. The best lactic-acid-producing strain was identified within the screening of CEN.PK m850 mutants.

Biotechnological advances in lactic acid production by lactic acid bacteria: lignocellulose as novel substrate

2018 ◽  
Vol 12 (2) ◽  
pp. 290-303 ◽  
Author(s):  
Enrique Cubas-Cano ◽  
Cristina González-Fernández ◽  
Mercedes Ballesteros ◽  
Elia Tomás-Pejó
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Production ◽  
Lactic Acid Production

Co-expression of two heterologous lactate dehydrogenases genes in Kluyveromyces marxianus for l -lactic acid production

2017 ◽  
Vol 241 ◽  
pp. 81-86 ◽  
Author(s):  
Jae Won Lee ◽  
Jung Hoon In ◽  
Joon-Bum Park ◽  
Jonghyeok Shin ◽  
Jin Hwan Park ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Kluyveromyces Marxianus ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Lactate Dehydrogenases

Lactic Acid Production from Distiller’s Grain by Mixed Ccultures of Lactobacillus casei and Streptococcus thermophilus

2010 ◽  
Vol 113-116 ◽  
pp. 1071-1074
Author(s):  
Hui Zou ◽  
Qun Hui Wang ◽  
Ying Ying Liu ◽  
Hui Li
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Synergistic Effects ◽  
Lactic Acid Production ◽  
Streptococcus Thermophilus ◽  
Fermentation Conditions ◽  
Distiller’S Grain ◽  
Distiller's Grain

Lactic acid production using Lactobacillus casei and Streptococcus thermophilus individually or as mixed culture on distiller’s grain in open fermentation conditions was evaluated. Lactic acid production and residual sugar were the main features examined. Increased lactic acid production was observed, when mixed cultures were used in comparison to individual ones. This revealed possible synergistic effects between the two lactic acid bacteria.

Identification and Characteristics of Lactic Acid Bacteria Isolated from Stylosanthes guianensis sw.

2011 ◽  
Vol 361-363 ◽  
pp. 701-706 ◽  
Author(s):  
Hai Rui Zhai ◽  
Han Lin Zhou ◽  
Jin Song Yang ◽  
Qian Wang ◽  
Hai Sheng Tan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
16S Rdna ◽  
Enterococcus Faecalis ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Hainan Province ◽  
Enterococcus Hirae ◽  
Stylosanthes Guianensis ◽  
Better Than

Four lactic acid bacteria (LAB) strains named HN78, HN87, HN91 and HN113 were separated fromStylosanthes guianensis SW. of Hainan province, which were identified bacterially using the classical classification and the sequences of 16S rDNA. The results showed that four strains wereEnterococcus hirae,Enterococcus faecalis,Lactococcus lactisandLactobacillus plantarum. The capability of lactic acid production of HN113 and HN91 were better than HN78 and HN87.

Sign in / Sign up

Export Citation Format

Share Document