Metabolic Alternations of Amino Acids, γ-Aminobutyric Acid, and Salicylic Acid in Solanum lycopersicum (L.) Following Preplanting Seedling Spray with Salicylic Acid

2018 ◽  
Vol 66 (46) ◽  
pp. 12236-12248 ◽  
Author(s):  
Hari C. Meher ◽  
Ghanendra Singh ◽  
Gautam Chawla
Keyword(s):  
Amino Acids ◽  
Salicylic Acid ◽  
Solanum Lycopersicum ◽  
Aminobutyric Acid ◽  
Solanum Lycopersicum L

scholarly journals A GC-ECD Method for Estimation of Free and Bound Amino Acids, γ-Aminobutyric Acid, Salicylic Acid, and Acetyl Salicylic Acid from Solanum lycopersicum (L.)

2011 ◽  
Vol 94 (1) ◽  
pp. 232-242 ◽  
Author(s):  
Hari Charan Meher ◽  
Vijay T Gajbhiye ◽  
Ghanendra Singh
Keyword(s):  
Amino Acids ◽  
Salicylic Acid ◽  
Solanum Lycopersicum ◽  
Iodoacetic Acid ◽  
Operating Conditions ◽  
Aminobutyric Acid ◽  
Acetyl Salicylic Acid ◽  
Electron Capture Detection ◽  
Acid Vapor ◽  
Solanum Lycopersicum L

Abstract A gas chromatograph with electron capture detection method for estimation of selected metabolites—amino acids (free and bound), γ-aminobutyric acid (GABA), salicylic acid (SA), and acetyl salicylic acid (ASA) from tomato—is reported. The method is based on nitrophenylation of the metabolites by 1-fluoro-2,4-dinitrobenzene under aqueous alkaline conditions to form dinitrophenyl derivatives. The derivatives were stable under the operating conditions of GC. Analysis of bound amino acids comprised perchloric acid precipitation of protein, alkylation (carboxymethylation) with iodoacetic acid, vapor-phase hydrolysis, and derivatization with 1-fluoro-2,4-dinitrobenzene in that order. The metabolites were resolved in 35 min, using a temperature-programmed run. The method is rapid, sensitive, and precise. It easily measured the typical amino acids (aspartate, asparagine, glutamate, glutamine, alanine, leucine, lysine, and phenylalanine) used for identification and quantification of a protein, resolved amino acids of the same mass (leucine and isoleucine), satisfactorily measured sulfur amino acids (methionine, cystine, and cysteine), and quantified GABA, SA, and ASA, as well. The developed method was validated for specificity, linearity, and precision. It has been applied and recommended for estimation of 25 metabolites from Solanum lycopersicum (L.).

Response of Tomato (Solanum lycopersicum L.) to Salicylic Acid and Calcium

2017 ◽  
Vol 15 (2) ◽  
pp. 1-7
Author(s):  
Nigar Afsana ◽  
Mohammad Islam ◽  
Elias Hossain ◽  
Rezowana Nizam ◽  
Nipa Monalesa ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L

scholarly journals Metabolic Alterations in Organic Acids and γ-Aminobutyric Acid in Developing Tomato (Solanum lycopersicum L.) Fruits

2010 ◽  
Vol 51 (8) ◽  
pp. 1300-1314 ◽  
Author(s):  
Yong-Gen Yin ◽  
Takehiro Tominaga ◽  
Yoko Iijima ◽  
Koh Aoki ◽  
Daisuke Shibata ◽  
...  
Keyword(s):  
Organic Acids ◽  
Solanum Lycopersicum ◽  
Aminobutyric Acid ◽  
Metabolic Alterations ◽  
Solanum Lycopersicum L

Accumulation Mechanism of γ-Aminobutyric Acid in Tomatoes (Solanum lycopersicum L.) under Low O2with and without CO2

2012 ◽  
Vol 60 (4) ◽  
pp. 1013-1019 ◽  
Author(s):  
Nobukazu Mae ◽  
Yoshio Makino ◽  
Seiichi Oshita ◽  
Yoshinori Kawagoe ◽  
Atsushi Tanaka ◽  
...  
Keyword(s):  
Solanum Lycopersicum ◽  
Aminobutyric Acid ◽  
Solanum Lycopersicum L ◽  
Accumulation Mechanism

A Liquid Chromatography Method for Determination of Selected Amino Acids, Coenzymes, Growth Regulators, and Vitamins from Cicer arietinum (L.) and Solanum lycopersicum (L.)

2012 ◽  
Vol 95 (4) ◽  
pp. 1142-1152 ◽  
Author(s):  
Hari Charan Meher ◽  
Vijay T Gajbhiye ◽  
Ghanendra Singh
Keyword(s):  
Amino Acids ◽  
Jasmonic Acid ◽  
Solanum Lycopersicum ◽  
Cicer Arietinum ◽  
Growth Regulators ◽  
Food Quality ◽  
Cicer Arietinum L ◽  
Solanum Lycopersicum L ◽  
Fat Soluble Vitamins

Abstract A bottleneck in crosstalk and QC research has been the quantification of diverse chemotypes in small amounts of tissue. An LC-UV method for estimating 28 selected metabolites of the regulatory network underlying growth, development, maintenance, vital functions, defense reactions, and food quality is reported. The method was based on binary gradient resolutions of the analytes in an RP C18 column. The mobile phase comprised solvent A [water + 0.1% trifluoroacetic acid (TFA)] and B (acetonitrile + 0.085% TFA at a flow rate of 1 mL/min. Twenty-three metabolites (selected amino acids, coenzymes, growth regulators, phenolic antioxidant, and water-soluble vitamins) were detected at 254 nm, and four fat-soluble vitamins at 280 nm. Jasmonic acid was quantified at 210 nm. The RSDs of peak area and retention time for each metabolite were <5.8%. The calibration graphs were linear with R2 values ranging from 0.98 to 0.99. The LODs (μg/mL) were about 0.01–1.0 for 23 metabolites quantified at 254 nm, 0.1–0.2 for fat-soluble vitamins, and 0.1 for jasmonic acid. The recoveries ranged from 80 to 105%, with RSDs of 2.8 to 11.2%. The method has been satisfactorily applied for determination of 28 metabolites from Cicer arietinum (L.) and Solanum lycopersicum (L.). It could be an alternative and competitive method of choice that can cheaply and easily perform routine analysis for food quality and targeted metabolomics of chickpea and tomato in response to stressors.

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