Key Amino Residues Determining Binding Activities of the Odorant Binding Protein AlucOBP22 to Two Host Plant Terpenoids of Apolygus lucorum

2019 ◽  
Vol 67 (21) ◽  
pp. 5949-5956 ◽  
Author(s):  
Hangwei Liu ◽  
Hongxia Duan ◽  
Qi Wang ◽  
Yong Xiao ◽  
Qian Wang ◽  
...  
Keyword(s):  
Host Plant ◽  
Binding Protein ◽  
Odorant Binding Protein ◽  
Apolygus Lucorum ◽  
Odorant Binding

Molecular and Functional Characterization of One Odorant-Binding Protein Gene OBP3 in Bemisia tabaci (Hemiptera: Aleyrodidae)

2019 ◽  
Author(s):  
Ran Wang ◽  
Yuan Hu ◽  
Peiling Wei ◽  
Cheng Qu ◽  
Chen Luo
Keyword(s):  
Host Plant ◽  
Bemisia Tabaci ◽  
Binding Protein ◽  
Insect Pest ◽  
Critical Role ◽  
Functional Characterization ◽  
Odorant Binding Protein ◽  
Binding Characteristics ◽  
And Function ◽  
Odorant Binding

Abstract Odorant binding proteins (OBPs) of insects play a critical role in chemical perceptions and choice of insect host plant. Bemisia tabaci is a notorious insect pest which can damage more than 600 plant species. In order to explore functions of OBPs in B. tabaci, here we investigated binding characteristics and function of odorant-binding protein 3 in B. tabaci (BtabOBP3). The results indicated that BtabOBP3 shows highly similar sequence with OBPs of other insects, including the typical signature motif of six cysteines. The recombinant BtabOBP3 protein was obtained, and the evaluation of binding affinities to tested volatiles of host plant was conducted, then the results indicated that β-ionone had significantly higher binding to BtabOBP3 among other tested plant volatiles. Furthermore, silencing of BtabOBP3 significantly altered choice behavior of B. tabaci to β-ionone. In conclusion, it has been demonstrated that BtabOBP3 exerts function as one carrier of β-ionone and the results could be contributed to reveal the mechanisms of choosing host plant in B. tabaci.

Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera: Miridae)

2013 ◽  
Vol 48 (3) ◽  
pp. 301-311 ◽  
Author(s):  
Ping Ji ◽  
Shao-Hua Gu ◽  
Jing-Tao Liu ◽  
Xiao-Qiang Zhu ◽  
Yu-Yuan Guo ◽  
...  
Keyword(s):  
Expression Profile ◽  
Profile Analysis ◽  
Binding Protein ◽  
Odorant Binding Protein ◽  
Apolygus Lucorum ◽  
Expression Profile Analysis ◽  
Odorant Binding

scholarly journals Responses to Host Plant Volatiles and Identification of Odorant Binding Protein and Chemosensory Protein Genes in Bradysia odoriphaga

ACS Omega ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 3800-3811 ◽  
Author(s):  
Yuxin Zhang ◽  
Yanping Ren ◽  
Xiaolan Wang ◽  
Yong Liu ◽  
Ningxin Wang
Keyword(s):  
Host Plant ◽  
Plant Volatiles ◽  
Binding Protein ◽  
Odorant Binding Protein ◽  
Host Plant Volatiles ◽  
Chemosensory Protein ◽  
Odorant Binding

Functional characterizations of one odorant binding protein and three chemosensory proteins from Apolygus lucorum (Meyer-Dur) (Hemiptera: Miridae) legs

2013 ◽  
Vol 59 (7) ◽  
pp. 690-696 ◽  
Author(s):  
Jin-Feng Hua ◽  
Shuai Zhang ◽  
Jin-Jie Cui ◽  
Dao-Jie Wang ◽  
Chun-Yi Wang ◽  
...  
Keyword(s):  
Binding Protein ◽  
Odorant Binding Protein ◽  
Chemosensory Proteins ◽  
Apolygus Lucorum ◽  
Odorant Binding

scholarly journals Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata (Lepidoptera: Crambidae)

2021 ◽  
Author(s):  
Hui Ai ◽  
Yuying Liu ◽  
Guangyan Long ◽  
Yuan Yuan ◽  
Shaopei Huang ◽  
...  
Keyword(s):  
Host Plant ◽  
Binding Protein ◽  
Butyl Ester ◽  
Acid Sites ◽  
Maruca Vitrata ◽  
Odorant Binding Protein ◽  
Olfactory Sensilla ◽  
Pod Borer ◽  
Legume Pod Borer ◽  
Odorant Binding

Abstract Insect olfaction system plays a key role in the foraging food, pollination, mating, oviposition, reproduction and other insect physiological behavior. Odorant binding protein are widely found in the various olfactory sensilla of different insect antennae and involved in chemical signals discrimination from natural environment. In this study, a novel OBP gene, MvitOBP3 is identified from the legume pod borer, Maruca vitrata, which it mainly harms important legume vegetables including cowpea, soybean and lablab bean. Real-time PCR results demonstrated that MvitOBP3 gene was abundantly expressed in the antennal tissue of M. vitrata, while low levels were distributed in the head, thorax, abdomen, leg and wing of adult moths. The recombinant OBP3 protein was purified using the prokaryotic expression and affinity chromatography system. Fluorescence competitive binding experiments indicated that that MvitOBP3 protein exhibited greater binding affinities with host-plant flower volatiles including Butanoic acid butyl ester, Limonene, 1H-indol-4-ol and 2-methyl-3-phenylpropanal, highlighting they may have attractant activities for the oviposition of female moths on the legume vegetables. Moreover, protein homology modeling and molecular docking analysis revealed that there are six amino acid sites of MvitOBP3 involved in the binding of the host-plant volatiles. These findings will further promote to understand the key role of odorant binding protein during host perception and oviposition of M. vitrata moths, which improve the efficiency of semiochemical-based prevention and monitoring for this pest in the legume vegetables field.

An odorant‐binding protein of Asian citrus psyllid, Diaphorina citri , participates in the response of host plant volatiles

2021 ◽  
Author(s):  
Xiao‐Qiang Liu ◽  
Hong‐Bo Jiang ◽  
Jia‐Yao Fan ◽  
Tian‐Yuan Liu ◽  
Li‐Wei Meng ◽  
...  
Keyword(s):  
Host Plant ◽  
Plant Volatiles ◽  
Binding Protein ◽  
Odorant Binding Protein ◽  
Asian Citrus Psyllid ◽  
Diaphorina Citri ◽  
Host Plant Volatiles ◽  
Odorant Binding

scholarly journals Functional characteristics of a novel odorant binding protein in the legume pod borer, Maruca vitrata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hui Ai ◽  
Yuying Liu ◽  
Guangyan Long ◽  
Yuan Yuan ◽  
Shaopei Huang ◽  
...  
Keyword(s):  
Host Plant ◽  
Binding Protein ◽  
Butyl Ester ◽  
Acid Sites ◽  
Maruca Vitrata ◽  
Odorant Binding Protein ◽  
Olfactory Sensilla ◽  
Pod Borer ◽  
Legume Pod Borer ◽  
Odorant Binding

AbstractInsect olfaction system plays a key role in the foraging food, pollination, mating, oviposition, reproduction and other insect physiological behavior. Odorant binding protein are widely found in the various olfactory sensilla of different insect antennae and involved in chemical signals discrimination from natural environment. In this study, a novel OBP gene, MvitOBP3 is identified from the legume pod borer, Maruca vitrata, which it mainly harms important legume vegetables including cowpea, soybean and lablab bean. Real-time PCR results demonstrated that MvitOBP3 gene was abundantly expressed in the antennal tissue of M. vitrata, while low levels were distributed in the head, thorax, abdomen, leg and wing of adult moths. The recombinant OBP3 protein was purified using the prokaryotic expression and affinity chromatography system. Fluorescence competitive binding experiments indicated that that MvitOBP3 protein exhibited greater binding affinities with host-plant flower volatiles including Butanoic acid butyl ester, Limonene, 1H-indol-4-ol and 2-methyl-3-phenylpropanal, highlighting they may have attractant activities for the oviposition of female moths on the legume vegetables. Moreover, protein homology modeling and molecular docking analysis revealed that there are six amino acid sites of MvitOBP3 involved in the binding of the host-plant volatiles. These findings will further promote to understand the key role of odorant binding protein during host perception and oviposition of M. vitrata moths, which improve the efficiency of semiochemical-based prevention and monitoring for this pest in the legume vegetables field.

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