Phenolic Breakdown Products of Cyanidin and Quercetin Contribute to Protection against Mitochondrial Impairment and Reactive Oxygen Species Generation in an In Vitro Model of Hepatocyte Steatosis

2019 ◽  
Vol 67 (22) ◽  
pp. 6241-6247 ◽  
Author(s):  
Hossein Rafiei ◽  
Kosar Omidian ◽  
Brian Bandy
Keyword(s):  
Reactive Oxygen Species ◽  
In Vitro Model ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitochondrial Impairment ◽  
Hepatocyte Steatosis ◽  
Vitro Model

scholarly journals Effects of uremic solutes on reactive oxygen species in vitro model systems as a possibility of support the renal function management

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Renata P Assis ◽  
Juliana FA Castro ◽  
Vânia O Gutierres ◽  
Carlos A Arcaro ◽  
Renata S Brotto ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Renal Function ◽  
In Vitro Model ◽  
Reactive Oxygen ◽  
Model Systems ◽  
Oxygen Species ◽  
Uremic Solutes ◽  
Function Management ◽  
Vitro Model

Sevoflurane Protects Rat Mixed Cerebrocortical Neuronal–Glial Cell Cultures against Transient Oxygen–Glucose Deprivation

2006 ◽  
Vol 105 (5) ◽  
pp. 990-998 ◽  
Author(s):  
Paula T. Canas ◽  
Lionel J. Velly ◽  
Christelle N. Labrande ◽  
Benjamin A. Guillet ◽  
Valérie Sautou-Miranda ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
In Vitro Model ◽  
Glutamate Uptake ◽  
Reactive Oxygen ◽  
Glucose Deprivation ◽  
Oxygen Species ◽  
Lactate Dehydrogenase Release ◽  
Vitro Model ◽  
Glial Cell Cultures

Background The purpose of this study was to clarify the role of glutamate and reactive oxygen species in sevoflurane-mediated neuroprotection on an in vitro model of ischemia-reoxygenation. Methods Mature mixed cerebrocortical neuronal-glial cell cultures, treated or not with increasing concentrations of sevoflurane, were exposed to 90 min combined oxygen-glucose deprivation (OGD) in an anaerobic chamber followed by reoxygenation. Cell death was quantified by lactate dehydrogenase release into the media and cell viability by reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium by mitochondrial succinate dehydrogenase. Extracellular concentrations of glutamate and glutamate uptake were assessed at the end of the ischemic injury by high-performance liquid chromatography and incorporation of L-[H]glutamate into cells, respectively. Free radical generation in cells was assessed 6 h after OGD during the reoxygenation period using 2',7'-dichlorofluorescin diacetate, which reacts with intracellular radicals to be converted to its fluorescent product, 2',7'-dichlorofluorescin, in cell cytosol. Results Twenty-four hours after OGD, sevoflurane, in a concentration-dependent manner, significantly reduced lactate dehydrogenase release and increased cell viability. At the end of OGD, sevoflurane was able to reduce the OGD-induced decrease in glutamate uptake. This effect was impaired in the presence of threo-3-methyl glutamate, a specific inhibitor of the glial transporter GLT1. Sevoflurane counteracted the increase in extracellular level of glutamate during OGD and the generation of reactive oxygen species during reoxygenation. Conclusion Sevoflurane had a neuroprotective effect in this in vitro model of ischemia-reoxygenation. This beneficial effect may be explained, at least in part, by sevoflurane-induced antiexcitotoxic properties during OGD, probably depending on GLT1, and by sevoflurane-induced decrease of reactive oxygen species generation during reoxygenation.

Surgery-Derived Reactive Oxygen Species Produced by Polymorphonuclear Leukocytes Promote Tumor Recurrence: Studies in an In Vitro Model

2007 ◽  
Vol 140 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Wilhelmina M.U. van Grevenstein ◽  
Arend G.J. Aalbers ◽  
Sander ten Raa ◽  
Wim Sluiter ◽  
Leo J. Hofland ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Polymorphonuclear Leukocytes ◽  
Tumor Recurrence ◽  
In Vitro Model ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Vitro Model

UV Light-Activated GdYVO4:Eu3+ Nanoparticles Induce Reactive Oxygen Species Generation in Leukocytes Without Affecting Erythrocytes In Vitro

2021 ◽  
Author(s):  
Anatolii Onishchenko ◽  
Valeriy Myasoedov ◽  
Svetlana Yefimova ◽  
Oksana Nakonechna ◽  
Volodymyr Prokopyuk ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Uv Light ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Oxygen Species

Novel Tetrazoles against Acanthamoeba castellanii Belonging to the T4 Genotype

Chemotherapy ◽  
2021 ◽  
Author(s):  
Yassmin Isse Wehelie ◽  
Naveed Ahmed Khan ◽  
Itrat Fatima ◽  
Areeba Anwar ◽  
Kanwal Kanwal ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Silver Nanoparticles ◽  
Reactive Oxygen Species Generation ◽  
Acanthamoeba Castellanii ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Species Determination ◽  
One Pot ◽  
Tetrazole Derivatives

Background: Acanthamoeba castellanii is a pathogenic free-living amoeba responsible for blinding keratitis and fatal granulomatous amoebic encephalitis. However, treatments are not standardized but can involve the use of amidines, biguanides, and azoles. Objectives: The aim of this study was to synthesize a variety of synthetic tetrazole derivatives and test their activities against A. castellanii. Methods: A series of novel tetrazole compounds were synthesized by one-pot method and characterized by NMR and mass spectroscopy. These compounds were subjected to amoebicidal, and cytotoxicity assays against A. castellanii belonging to the T4 genotype and human keratinocyte skin cells respectively. Additionally, reactive oxygen species determination and electron microscopy studies were carried out. Furthermore, two of the seven compounds were conjugated with silver nanoparticles to study their antiamoebic potential. Results: A series of seven tetrazole derivatives were synthesized successfully. The selected tetrazoles showed anti-amoebic activities at 10µM concentration against A. castellanii in vitro. The compounds tested caused increased reactive oxygen species generation in A castellanii, and significant morphological damage to amoebal membranes. Moreover, conjugation of silver nanoparticles enhanced antiamoebic effects of two tetrazoles. Conclusions: The results showed that azole compounds hold promise in the development of new formulations of anti-Acanthamoebic agents.

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