scholarly journals Monohydrazone Based G-Quadruplex Selective Ligands Induce DNA Damage and Genome Instability in Human Cancer Cells

2020 ◽  
Vol 63 (6) ◽  
pp. 3090-3103 ◽  
Author(s):  
Jussara Amato ◽  
Giulia Miglietta ◽  
Rita Morigi ◽  
Nunzia Iaccarino ◽  
Alessandra Locatelli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Genome Instability ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
G Quadruplex ◽  
Selective Ligands

scholarly journals DNA damage and genome instability by G-quadruplex ligands are mediated by R loops in human cancer cells

2018 ◽  
Vol 116 (3) ◽  
pp. 816-825 ◽  
Author(s):  
Alessio De Magis ◽  
Stefano G. Manzo ◽  
Marco Russo ◽  
Jessica Marinello ◽  
Rita Morigi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Genome Instability ◽  
Human Cancer ◽  
Cell Killing ◽  
Dna Structures ◽  
Human Cancer Cells ◽  
Cellular Effects ◽  
Genome Wide ◽  
G Quadruplex

G quadruplexes (G4s) and R loops are noncanonical DNA structures that can regulate basic nuclear processes and trigger DNA damage, genome instability, and cell killing. By different technical approaches, we here establish that specific G4 ligands stabilize G4s and simultaneously increase R-loop levels within minutes in human cancer cells. Genome-wide mapping of R loops showed that the studied G4 ligands likely cause the spreading of R loops to adjacent regions containing G4 structures, preferentially at 3′-end regions of expressed genes, which are partially ligand-specific. Overexpression of an exogenous human RNaseH1 rescued DNA damage induced by G4 ligands in BRCA2-proficient and BRCA2-silenced cancer cells. Moreover, even if the studied G4 ligands increased noncanonical DNA structures at similar levels in nuclear chromatin, their cellular effects were different in relation to cell-killing activity and stimulation of micronuclei, a hallmark of genome instability. Our findings therefore establish that G4 ligands can induce DNA damage by an R loop-dependent mechanism that can eventually lead to different cellular consequences depending on the chemical nature of the ligands.

Abstract 4838: R loop-driven genome instability by G-quadruplex binders in BRCA2-silenced human cancer cells

2018 ◽  
Author(s):  
Alessio De Magis ◽  
Stefano Giustino Manzo ◽  
Marco Russo ◽  
Olivier Sordet ◽  
Rita Morigi ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Genome Instability ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
G Quadruplex

scholarly journals Withanolide D Enhances Radiosensitivity of Human Cancer Cells by Inhibiting DNA Damage Non-homologous End Joining Repair Pathway

2020 ◽  
Vol 9 ◽  
Author(s):  
Jerome Lacombe ◽  
Titouan Cretignier ◽  
Laetitia Meli ◽  
E. M. Kithsiri Wijeratne ◽  
Jean-Luc Veuthey ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Human Cancer ◽  
Repair Pathway ◽  
End Joining ◽  
Human Cancer Cells ◽  
Non Homologous End Joining

scholarly journals Detection of repair activity during the DNA damage-induced G2 delay in human cancer cells

Oncogene ◽  
2001 ◽  
Vol 20 (27) ◽  
pp. 3486-3496 ◽  
Author(s):  
Gary D Kao ◽  
W Gillies McKenna ◽  
Tim J Yen
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
Repair Activity ◽  
G2 Delay

Study of the biological effects and DNA damage exerted by a new dipalladium-Hmtpo complex on human cancer cells

2002 ◽  
Vol 90 (1-2) ◽  
pp. 51-60 ◽  
Author(s):  
Khalid Akdi ◽  
Rosario A. Vilaplana ◽  
Sanaa Kamah ◽  
Jorge A.R. Navarro ◽  
Juan M. Salas ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Human Cancer ◽  
Biological Effects ◽  
Human Cancer Cells

Differential response between the p53 ubiquitin–protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells

2006 ◽  
Vol 312 (17) ◽  
pp. 3370-3378 ◽  
Author(s):  
Wenrui Duan ◽  
Li Gao ◽  
Xin Wu ◽  
Yang Zhang ◽  
Gregory A. Otterson ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Human Cancer ◽  
Differential Response ◽  
Human Cancer Cells ◽  
Protein Ligases
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