scholarly journals Serum Bile Acid Profiles Improve Clinical Prediction of Nonalcoholic Fatty Liver in T2DM patients

2021 ◽  
Author(s):  
Tao Wu ◽  
Ming Yang ◽  
Hanchen Xu ◽  
Lei Wang ◽  
Huafeng Wei ◽  
...  
Keyword(s):  
Bile Acid ◽  
Fatty Liver ◽  
Serum Bile Acid ◽  
Clinical Prediction ◽  
Nonalcoholic Fatty Liver ◽  
Bile Acid Profiles

scholarly journals Serum Bile Acid Profiles Improve Clinical Prediction of Nonalcoholic Fatty Liver in T2DM patients

2020 ◽  
Author(s):  
Tao Wu ◽  
Ming Yang ◽  
Hanchen Xu ◽  
Lei Wang ◽  
Huafeng Wei ◽  
...  
Keyword(s):  
Bile Acid ◽  
Fatty Liver ◽  
Area Under The Curve ◽  
Roc Curves ◽  
Serum Bile Acid ◽  
Nonalcoholic Fatty Liver ◽  
Clinical Variables ◽  
Net Reclassification Improvement ◽  
Bile Acid Profiles ◽  
First Time

Abstract Background: The present study aimed to assess the ability of serum bile acid profiles to predict the development of nonalcoholic fatty liver (NAFL) in type 2 diabetes mellitus (T2DM) patients. Methods: Using targeted ultraperformance liquid chromatography (UPLC) coupled with triple quadrupole mass spectrometry (TQ/MS), we compared serum bile acid levels in T2DM patients with NAFL (n=30) and age-gender matched T2DM patients without NAFL (n=36) at the first time. Second, an independent cohort study of T2DM patients with NAFL (n=17) and age-gender matched T2DM patients without NAFL (n=20) was used to validate the results. The incremental benefits of serum biomarkers, clinical variables alone or with biomarkers were then evaluated using receiver operating characteristic (ROC) curves and decision curve analysis. Area under the curve (AUC), integrated discrimination improvement (IDI) and net reclassification improvement (NRI) were used to evaluate the biomarker predictive abilities. Results: The serum bile acid profiles in T2DM patients with NAFL were significantly different from T2DM patients without NAFL, as characterized by the significant elevation of LCA, TLCA, TUDCA, CDCA24G and TCDCA, which may be potential biomarkers for identification of NAFL in T2DM. Based on the improvement in AUC, IDI and NRI, addition of 5 bile acids to a model with clinical variables statistically improved its predictive value. Similar results were found in the validation cohort.Conclusions: These results highlight detected biomarkers may contribute to the progression of NAFL in T2DM patients, and these biomarkers particularly in combination may help diagnosis of NAFL and allow earlier intervention in T2DM patients.

scholarly journals Disease-Associated Gut Microbiota Reduces the Profile of Secondary Bile Acids in Pediatric Nonalcoholic Fatty Liver Disease

2021 ◽  
Vol 11 ◽  
Author(s):  
Jiake Yu ◽  
Hu Zhang ◽  
Liya Chen ◽  
Yufei Ruan ◽  
Yiping Chen ◽  
...  
Keyword(s):  
Liver Disease ◽  
Bile Acid ◽  
Gut Microbiota ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Bile Acids ◽  
Fatty Liver Disease ◽  
Healthy Children ◽  
Nonalcoholic Fatty Liver ◽  
Secondary Bile Acids

Children with nonalcoholic fatty liver disease (NAFLD) display an altered gut microbiota compared with healthy children. However, little is known about the fecal bile acid profiles and their association with gut microbiota dysbiosis in pediatric NAFLD. A total of 68 children were enrolled in this study, including 32 NAFLD patients and 36 healthy children. Fecal samples were collected and analyzed by metagenomic sequencing to determine the changes in the gut microbiota of children with NAFLD, and an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) system was used to quantify the concentrations of primary and secondary bile acids. The associations between the gut microbiota and concentrations of primary and secondary bile acids in the fecal samples were then analyzed. We found that children with NAFLD exhibited reduced levels of secondary bile acids and alterations in bile acid biotransforming-related bacteria in the feces. Notably, the decrease in Eubacterium and Ruminococcaceae bacteria, which express bile salt hydrolase and 7α-dehydroxylase, was significantly positively correlated with the level of fecal lithocholic acid (LCA). However, the level of fecal LCA was negatively associated with the abundance of the potential pathogen Escherichia coli that was enriched in children with NAFLD. Pediatric NAFLD is characterized by an altered profile of gut microbiota and fecal bile acids. This study demonstrates that the disease-associated gut microbiota is linked with decreased concentrations of secondary bile acids in the feces. The disease-associated gut microbiota likely inhibits the conversion of primary to secondary bile acids.

Potential Implications for Monitoring Serum Bile Acid Profiles in Circulation with Serum Proteome for Carbon Tetrachloride-Induced Liver Injury/Regeneration Model in Mice

2010 ◽  
Vol 9 (9) ◽  
pp. 4490-4500 ◽  
Author(s):  
Takashi Shimada ◽  
Tsuyoshi Nakanishi ◽  
Atsuhiko Toyama ◽  
Satoshi Yamauchi ◽  
Atsuhiro Kanzaki ◽  
...  
Keyword(s):  
Bile Acid ◽  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Serum Bile Acid ◽  
Serum Proteome ◽  
Bile Acid Profiles

scholarly journals Role of Bile Acids in Dysbiosis and Treatment of Nonalcoholic Fatty Liver Disease

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Caihua Wang ◽  
Chunpeng Zhu ◽  
Liming Shao ◽  
Jun Ye ◽  
Yimin Shen ◽  
...  
Keyword(s):  
Liver Disease ◽  
Bile Acid ◽  
Gut Microbiota ◽  
Fatty Liver ◽  
Nonalcoholic Fatty Liver Disease ◽  
Bile Acids ◽  
Fatty Liver Disease ◽  
Nonalcoholic Fatty Liver ◽  
Bile Acid Receptors

Nonalcoholic fatty liver disease (NAFLD) is a major health threat around the world and is characterized by dysbiosis. Primary bile acids are synthesized in the liver and converted into secondary bile acids by gut microbiota. Recent studies support the role of bile acids in modulating dysbiosis and NAFLD, while the mechanisms are not well elucidated. Dysbiosis may alter the size and the composition of the bile acid pool, resulting in reduced signaling of bile acid receptors such as farnesoid X receptor (FXR) and Takeda G protein-coupled receptor 5 (TGR5). These receptors are essential in lipid and glucose metabolism, and impaired bile acid signaling may cause NAFLD. Bile acids also reciprocally regulate the gut microbiota directly via antibacterial activity and indirectly via FXR. Therefore, bile acid signaling is closely linked to dysbiosis and NAFLD. During the past decade, stimulation of bile acid receptors with their agonists has been extensively explored for the treatment of NAFLD in both animal models and clinical trials. Early evidence has suggested the potential of bile acid receptor agonists in NAFLD management, but their long-term safety and effectiveness need further clarification.

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