Determination of the Aggregation Number of Pyrene-Labeled Gemini Surfactant Micelles by Pyrene Fluorescence Quenching Measurements

Alcohols are very important additives and played a measure role as co-surfactants in various industrial and research applications. While, Gemini surfactants are mainly used as effective emulsifiers, antifoaming agents, bactericidal agents, coating agents and corrosion inhibitors etc. Therefore, it is important to study about the variations in alkanol concentrations which affects the aggregation number and other related parameters. Surface properties of gemini surfactant butanediyl-1,4-bis(dimethyldodecylammonium bromide) (12-4-12) has been studied by using fluorescence method. This method has been used to calculate the aggregation number (Nagg) and the other related parameters like dielectric constant (D), Stern Volmer binding constant (KSV) of mixed system. This method is also very important for the calculation of the micropolarity of the mixed system (gemini/alcohol). The micropolarity has been obtained with the help of the ratio of intensity of peaks (I1/I3) of the pyrene fluorescence emission spectrum. Cetylpridinium chloride and pyrene were used as quencher and probe, respectively. Keywords: Gemini surfactant, special alcohols, aggregation number, micropolarity

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Determination of the aggregation number of detergent micelles using steady-state fluorescence quenching

Biophysical Journal ◽

10.1016/s0006-3495(93)81528-5 ◽

1993 ◽

Vol 64 (5) ◽

pp. 1580-1587 ◽

Cited By ~ 65

Author(s):

P.J. Tummino ◽

A. Gafni

Keyword(s):

Steady State ◽

Fluorescence Quenching ◽

Aggregation Number ◽

Steady State Fluorescence ◽

Detergent Micelles

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Synthesis and Characterization of Dicationic Gemini Surfactant Micelles and their Effect on the Rate of Ninhydrin–Copper-Peptide Complex Reaction

Tenside Surfactants Detergents ◽

10.3139/113.110535 ◽

2018 ◽

Vol 55 (1) ◽

pp. 78-84 ◽

Cited By ~ 41

Author(s):

Dileep Kumar ◽

Malik Abdul Rub

Keyword(s):

Gemini Surfactant ◽

Synthesis And Characterization ◽

Complex Reaction ◽

Surfactant Micelles ◽

Peptide Complex

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A Homogeneous Nonisotopic Histone Deacetylase Activity Assay

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057102239644 ◽

2003 ◽

Vol 8 (1) ◽

pp. 89-95 ◽

Cited By ~ 21

Author(s):

Birgit Heltweg ◽

Manfred Jung

Keyword(s):

Drug Discovery ◽

Fluorescence Quenching ◽

Histone Deacetylase ◽

Anticancer Agents ◽

Histone Deacetylases ◽

Activity Assay ◽

Animal Testing ◽

Hdac Activity

Histone deacetylases (HDACs) are important regulators of transcription, and their inhibitors are a promising class of anticancer agents. The methods for the determination of HDAC activity and its inhibition that are currently available suffer from various drawbacks, such as animal testing, radioactive substrates, or limited throughput. Therefore, a fast nonisotopic method for the measurement of HDAC activity is highly desirable. The authors present such an assay that relies on the fluorescent HDAC substrate developed previously in their group. After incubation of the substrate with the enzyme, a derivatization leads to efficient fluorescence quenching in the deacetylated metabolite. Thus, only the fluorescence emitted by the remaining substrate is detected, which allows for a convenient detection of HDAC activity in a homogeneous format that can be performed on multiwell plate readers. This procedure, called HDASH (histone deacetylase assay—homogeneous), should be a valuable tool in transcriptional research and especially drug discovery. ( Journal of Biomolecular Screening 2003:89-95)

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