Determination of Sodium Hexametaphosphate in Beverages Based on the Fluorescence Quenching of Acridine Orange

2014 ◽  
Vol 47 (16) ◽  
pp. 2740-2746
Author(s):  
Heng Xin Zhao ◽  
Ming Luo ◽  
Li Xin Mo ◽  
Liang Cheng ◽  
Zheng Ma ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Acridine Orange ◽  
Sodium Hexametaphosphate

A Homogeneous Nonisotopic Histone Deacetylase Activity Assay

2003 ◽  
Vol 8 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Birgit Heltweg ◽  
Manfred Jung
Keyword(s):  
Drug Discovery ◽  
Fluorescence Quenching ◽  
Histone Deacetylase ◽  
Anticancer Agents ◽  
Histone Deacetylases ◽  
Activity Assay ◽  
Animal Testing ◽  
Hdac Activity

Histone deacetylases (HDACs) are important regulators of transcription, and their inhibitors are a promising class of anticancer agents. The methods for the determination of HDAC activity and its inhibition that are currently available suffer from various drawbacks, such as animal testing, radioactive substrates, or limited throughput. Therefore, a fast nonisotopic method for the measurement of HDAC activity is highly desirable. The authors present such an assay that relies on the fluorescent HDAC substrate developed previously in their group. After incubation of the substrate with the enzyme, a derivatization leads to efficient fluorescence quenching in the deacetylated metabolite. Thus, only the fluorescence emitted by the remaining substrate is detected, which allows for a convenient detection of HDAC activity in a homogeneous format that can be performed on multiwell plate readers. This procedure, called HDASH (histone deacetylase assay—homogeneous), should be a valuable tool in transcriptional research and especially drug discovery. ( Journal of Biomolecular Screening 2003:89-95)

An “on–off” fluorescent probe based on cucurbit[7]uril for highly sensitive determination of ammonia nitrogen in aquaculture water

2021 ◽  
Author(s):  
Zhen Li ◽  
Tan Wang ◽  
Xianbao Xu ◽  
Cong Wang ◽  
Daoliang Li
Keyword(s):  
Fluorescence Quenching ◽  
Inclusion Complex ◽  
Fluorescent Probe ◽  
Ammonia Nitrogen ◽  
Highly Sensitive ◽  
Sensitive Determination ◽  
Aquaculture Water

A novel “on–off” fluorescent probe for the determination of ammonia nitrogen has been synthesized. URO can replace PAL into the cavity of CB[7] to form a stable inclusion complex, eventually forming the fluorescence quenching system of URO@CB[7].

Na+/H+ antiporter of brush border vesicles: studies with acridine orange uptake

1982 ◽  
Vol 242 (6) ◽  
pp. F733-F739 ◽  
Author(s):  
D. G. Warnock ◽  
W. W. Reenstra ◽  
V. J. Yee
Keyword(s):  
Fluorescence Quenching ◽  
Acridine Orange ◽  
Brush Border ◽  
Membrane Vesicles ◽  
Sodium Concentration ◽  
Sodium Influx ◽  
Ph Gradients ◽  
Orange Fluorescence ◽  
Acridine Orange Fluorescence ◽  
Rate Limiting

Fluorescence quenching of acridine orange was used to characterize the generation and collapse of pH gradients by the Na+/H+ antiporter of brush border membrane vesicles prepared from rabbit renal cortex. Quenching was observed when acridine orange, a weak base, was taken up to an acidic intravesicular space. Na+/H+ exchange was examined with both Na+ uptake and efflux studies. Acridine orange fluorescence quenching demonstrated the cation specificity of the Na+/H+ antiporter (i.e., sodium and lithium) and was inhibited by amiloride. Parallel studies with nigericin, a K+/H+ antiporter, demonstrated that acridine orange responded very rapidly to pH gradients. Therefore, acridine orange equilibration was not rate limiting in our studies of the Na+/H+ antiporter. Initial rate measurements were made to obtain kinetic parameters for the Na+/H+ antiporter. In sodium influx studies, the half-maximal rate of acridine orange fluorescence change was obtained with an external sodium concentration of 13.3 +/- 0.5 mM.

Determination of lateral diffusion coefficients in air-water monolayers by fluorescence quenching measurements

1991 ◽  
Vol 113 (13) ◽  
pp. 4838-4843 ◽  
Author(s):  
F. Caruso ◽  
F. Grieser ◽  
A. Murphy ◽  
P. Thistlethwaite ◽  
R. Urquhart ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Diffusion Coefficients ◽  
Lateral Diffusion
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