High-Throughput Screening for Colloidal Stability of Peptide Formulations Using Dynamic and Static Light Scattering

2021 ◽  
Author(s):  
Katharina Dauer ◽  
Walter Kamm ◽  
Karl Gerhard Wagner ◽  
Stefania Pfeiffer-Marek
Keyword(s):  
Light Scattering ◽  
High Throughput ◽  
High Throughput Screening ◽  
Colloidal Stability ◽  
Static Light Scattering

Simultaneous in-Situ Monitoring of Parallel Polymerization Reactions Using Light Scattering; A New Tool for High-Throughput Screening

2004 ◽  
Vol 6 (5) ◽  
pp. 710-716 ◽  
Author(s):  
Michael F. Drenski ◽  
Emmanuel Mignard ◽  
Alina M. Alb ◽  
Wayne F. Reed
Keyword(s):  
Light Scattering ◽  
High Throughput ◽  
High Throughput Screening ◽  
In Situ Monitoring

scholarly journals Microwell Plate-Based Dynamic Light Scattering as a High-Throughput Characterization Tool in Biopharmaceutical Development

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 172
Author(s):  
Katharina Dauer ◽  
Stefania Pfeiffer-Marek ◽  
Walter Kamm ◽  
Karl G. Wagner
Keyword(s):  
Light Scattering ◽  
High Throughput ◽  
Colloidal Stability ◽  
Active Pharmaceutical Ingredient ◽  
Formulation Development ◽  
Drug Candidates ◽  
Coefficients Of Variation ◽  
Kd Values ◽  
Data Points ◽  
Lower Variability

High-throughput light scattering instruments are widely used in screening of biopharmaceutical formulations and can be easily incorporated into processes by utilizing multi-well plate formats. High-throughput plate readers are helpful tools to assess the aggregation tendency and colloidal stability of biological drug candidates based on the diffusion self-interaction parameter (kD). However, plate readers evoke issues about the precision and variability of determined data. In this article, we report about the statistical evaluation of intra- and inter-plate variability (384-well plates) for the kD analysis of protein and peptide solutions. ANOVA revealed no significant differences between the runs. In conclusion, the reliability and precision of kD was dependent on the plate position of the sample replicates and kD value. Positive kD values (57.0 mL/g, coefficients of variation (CV) 8.9%) showed a lower variability compared to negative kD values (−14.8 mL/g, CV 13.4%). The variability of kD was not reduced using more data points (120 vs. 30). A kD analysis exclusively based on center wells showed a lower CV (<2%) compared to edge wells (5–12%) or a combination of edge and center wells (2–5%). We present plate designs for kD analysis within the early formulation development, screening up to 20 formulations consuming less than 50 mg of active pharmaceutical ingredient (API).

A polarization sensitive light scattering unit for high throughput screening

2018 ◽  
Vol 89 (11) ◽  
pp. 113109
Author(s):  
John F. Anderson ◽  
Michael F. Drenski ◽  
Curtis W. Jarand ◽  
Anton Dudko ◽  
Wayne F. Reed
Keyword(s):  
Light Scattering ◽  
High Throughput ◽  
High Throughput Screening

A Colloidal Stability Assay Suitable for High-Throughput Screening

2016 ◽  
Vol 88 (5) ◽  
pp. 2929-2936 ◽  
Author(s):  
Carla Abarca ◽  
M. Monsur Ali ◽  
Songtao Yang ◽  
Xiaofei Dong ◽  
Robert H. Pelton
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Colloidal Stability

scholarly journals In vitro optoacoustic flow cytometry with light scattering referencing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Markus Seeger ◽  
Andre C. Stiel ◽  
Vasilis Ntziachristos
Keyword(s):  
Light Scattering ◽  
Directed Evolution ◽  
High Throughput ◽  
High Throughput Screening ◽  
Single Cells ◽  
Light Flux ◽  
Focal Volume ◽  
Optoacoustic Imaging ◽  
Excitation Light

AbstractMorphological and functional optoacoustic imaging is enhanced by dedicated transgene reporters, in analogy to fluorescence methods. The development of optoacoustic reporters using protein engineering and directed evolution would be accelerated by high-throughput in-flow screening for intracellular, genetically encoded, optoacoustic contrast. However, accurate characterization of such contrast is impeded because the optoacoustic signals depend on the cell’s size and position in the flow chamber. We report herein an optoacoustic flow cytometer (OA-FCM) capable of precise measurement of intracellular optoacoustic signals of genetically-encoded chromoproteins in flow. The novel system records light-scattering as a reference for the detected optoacoustic signals in order to account for cell size and position, as well as excitation light flux in the focal volume, which we use to reference the detected optoacoustic signals to enhance the system’s precision. The OA-FCM was calibrated using micrometer-sized particles to showcase the ability to assess in-flow objects in the size range of single-cells. We demonstrate the capabilities of our OA-FCM to identify sub-populations in a mixture of two E. coli stocks expressing different reporter-proteins with a precision of over 90%. High-throughput screening of optoacoustic labels could pave the way for identifying genetically encoded optoacoustic reporters by transferring working concepts of the fluorescence field such as directed evolution and activated cell sorting.

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts

High throughput screening of microbial biodiversity for the discovery of novel cosmeceutical agents

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
K Georgousaki ◽  
N DePedro ◽  
AM Chinchilla ◽  
N Aliagiannis ◽  
F Vicente ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microbial Biodiversity
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