Simultaneous in-Situ Monitoring of Parallel Polymerization Reactions Using Light Scattering; A New Tool for High-Throughput Screening

2004 ◽  
Vol 6 (5) ◽  
pp. 710-716 ◽  
Author(s):  
Michael F. Drenski ◽  
Emmanuel Mignard ◽  
Alina M. Alb ◽  
Wayne F. Reed
Keyword(s):  
Light Scattering ◽  
High Throughput ◽  
High Throughput Screening ◽  
In Situ Monitoring

scholarly journals Integration of an In Situ MALDI-Based High-Throughput Screening Process: A Case Study with Receptor Tyrosine Kinase c-MET

2017 ◽  
Vol 22 (10) ◽  
pp. 1203-1210 ◽  
Author(s):  
Katrin Beeman ◽  
Jens Baumgärtner ◽  
Manuel Laubenheimer ◽  
Karlheinz Hergesell ◽  
Martin Hoffmann ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Maldi Ms ◽  
Kinase Assay ◽  
Label Free ◽  
Screening Process ◽  
Label Free Detection ◽  
Ic50 Values

Mass spectrometry (MS) is known for its label-free detection of substrates and products from a variety of enzyme reactions. Recent hardware improvements have increased interest in the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for high-throughput drug discovery. Despite interest in this technology, several challenges remain and must be overcome before MALDI-MS can be integrated as an automated “in-line reader” for high-throughput drug discovery. Two such hurdles include in situ sample processing and deposition, as well as integration of MALDI-MS for enzymatic screening assays that usually contain high levels of MS-incompatible components. Here we adapt our c-MET kinase assay to optimize for MALDI-MS compatibility and test its feasibility for compound screening. The pros and cons of the Echo (Labcyte) as a transfer system for in situ MALDI-MS sample preparation are discussed. We demonstrate that this method generates robust data in a 1536-grid format. We use the MALDI-MS to directly measure the ratio of c-MET substrate and phosphorylated product to acquire IC50 curves and demonstrate that the pharmacology is unaffected. The resulting IC50 values correlate well between the common label-based capillary electrophoresis and the label-free MALDI-MS detection method. We predict that label-free MALDI-MS-based high-throughput screening will become increasingly important and more widely used for drug discovery.

In situ monitoring the role of citrate in chemical bath deposition of PbS thin films

CrystEngComm ◽  
2016 ◽  
Vol 18 (1) ◽  
pp. 149-156 ◽  
Author(s):  
Sucheta Sengupta ◽  
Maayan Perez ◽  
Alexander Rabkin ◽  
Yuval Golan
Keyword(s):  
Thin Films ◽  
Light Scattering ◽  
Optical Absorption ◽  
Chemical Bath Deposition ◽  
Trisodium Citrate ◽  
In Situ Monitoring ◽  
Cluster Mechanism ◽  
Absorption Measurements

We report the formation of size tunable PbS nanocubes induced by the presence of trisodium citrate during growth of PbS thin films by chemical bath deposition. The presence of citrate induces growth by the cluster mechanism which is monitored by XRD and HRSEM, along with real time light scattering and optical absorption measurements.

In-situ nanoelectrospray for high-throughput screening of enzymes and real-time monitoring of reactions

2016 ◽  
Vol 902 ◽  
pp. 135-141 ◽  
Author(s):  
Yuhan Yang ◽  
Feifei Han ◽  
Jin Ouyang ◽  
Yunling Zhao ◽  
Juan Han ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
High Throughput Screening ◽  
Real Time Monitoring

Preparation and In‐Situ High‐Throughput Screening of Bi Thixotropic Ink

2021 ◽  
pp. 2101691
Author(s):  
Lei Yao ◽  
Zhuoming Xu ◽  
Junhao Qiu ◽  
Jian Zhang ◽  
Dongwang Yang ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening
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