Label-Free Imaging of Flap Endonuclease 1 in Living Cells by Assembling Original and Multifunctional Nanoprobe

2020 ◽  
Vol 3 (7) ◽  
pp. 4573-4580 ◽  
Author(s):  
Chenchen Wang ◽  
Duoduo Zhang ◽  
Yunfei Tang ◽  
Wei Wei ◽  
Yong Liu ◽  
...  
Keyword(s):  
Living Cells ◽  
Label Free ◽  
Flap Endonuclease 1 ◽  
Multifunctional Nanoprobe

Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

2011 ◽  
Vol 6 (11) ◽  
pp. 1748-1760 ◽  
Author(s):  
Ralf Schröder ◽  
Johannes Schmidt ◽  
Stefanie Blättermann ◽  
Lucas Peters ◽  
Nicole Janssen ◽  
...  
Keyword(s):  
G Protein ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Label Free ◽  
Dynamic Mass ◽  
Mass Redistribution ◽  
G Protein Coupled ◽  
Free Dynamic

scholarly journals Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

10.3791/58486 ◽  
2018 ◽  
Author(s):  
André Gemeinhardt ◽  
Matthew P. McDonald ◽  
Katharina König ◽  
Michael Aigner ◽  
Andreas Mackensen ◽  
...  
Keyword(s):  
Living Cells ◽  
Label Free

scholarly journals Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 102 ◽  
Author(s):  
Richard Schasfoort ◽  
Fikri Abali ◽  
Ivan Stojanovic ◽  
Gestur Vidarsson ◽  
Leon Terstappen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mammalian Cells ◽  
Living Cells ◽  
Label Free ◽  
Intact Cells ◽  
Cell Parameters ◽  
Spr Imaging ◽  
Label Free Detection

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.

scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

2018 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Living Cells ◽  
Transmitted Light ◽  
Integrated Systems ◽  
Label Free ◽  
Subcellular Structure ◽  
Modern Biology ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

scholarly journals Methods and applications of label-free cell-based systems

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Joachim Wiest
Keyword(s):  
Environmental Monitoring ◽  
Morphological Changes ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Controlled System ◽  
Chip Technology ◽  
Cell Monitoring ◽  
A Cell ◽  
On Chip

Label-free monitoring of living cells is used in various applications such as drug development, toxicology, regenerative medicine or environmental monitoring. The most prominent methods for monitoring the extracellular acidification, oxygen consumption, electrophysiological activity and morphological changes of living cells are described. Furthermore, the intelligent mobile lab (IMOLA) – a computer controlled system integrating cell monitoring and automated cell cultivation – is described as an example of a cell-based system for microphysiometry. Results from experiments in the field of environmental monitoring using algae are presented. An outlook toward the development of an organ-on-chip technology is given.

The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems

2004 ◽  
Vol 25 (21-22) ◽  
pp. 3740-3745 ◽  
Author(s):  
Jurjen Emmelkamp ◽  
Floor Wolbers ◽  
Helene Andersson ◽  
Ralph S. DaCosta ◽  
Brian C. Wilson ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Microfluidic Systems ◽  
Single Living Cells ◽  
Single Living

A label-free multifunctional nanosensor based on N-doped carbon nanodots for vitamin B12 and Co2+ detection, and bioimaging in living cells and zebrafish

2020 ◽  
Vol 8 (23) ◽  
pp. 5089-5095
Author(s):  
Fangfang Du ◽  
Zhe Cheng ◽  
Marius Kremer ◽  
Yang Liu ◽  
Xiaodong Wang ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
A549 Cell ◽  
Living Cells ◽  
Carbon Nanodots ◽  
Label Free ◽  
Synthetic Procedure ◽  
Fluorescence Nanoprobe ◽  
Co2 Detection

Illustration of the synthetic procedure of N-CNDs and N-CDs-based bifunctional fluorescence nanoprobe for the detection of VB12 and Co2+ and bioimaging of A549 cell and zebrafish.

scholarly journals High-resolution Label-free 3D Mapping of metabolites of Single Living Cells

2020 ◽  
Vol 26 (S2) ◽  
pp. 2990-2992
Author(s):  
Alexander Erofeev ◽  
Petr Gorelkin ◽  
Pavel Novak ◽  
Christopher Edwards ◽  
Andrew Shevchuk ◽  
...  
Keyword(s):  
High Resolution ◽  
Living Cells ◽  
3D Mapping ◽  
Label Free ◽  
Single Living Cells ◽  
Single Living

Single-layer MnO2 nanosheet quenched fluorescence ruthenium complexes for sensitive detection of ferrous iron

RSC Advances ◽  
2016 ◽  
Vol 6 (82) ◽  
pp. 79204-79208 ◽  
Author(s):  
Xing He ◽  
Xiaoxiao Yang ◽  
Luo Hai ◽  
Dinggeng He ◽  
Xiaoxiao He ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Ferrous Iron ◽  
Ruthenium Complexes ◽  
Single Layer ◽  
Living Cells ◽  
Sensitive Detection ◽  
Label Free ◽  
Fluorescence Sensors ◽  
Turn On ◽  
Mno2 Nanosheet

Single-layer MnO2 nanosheet quenched fluorescence Ru(bipy)32+ complexes are established as turn-on fluorescence sensors for sensitive and label-free probing of ferrous iron in aqueous solutions, as well as living cells.

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