scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

2018 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Living Cells ◽  
Transmitted Light ◽  
Integrated Systems ◽  
Label Free ◽  
Subcellular Structure ◽  
Modern Biology ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted-light microscopy

2018 ◽  
Vol 15 (11) ◽  
pp. 917-920 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Light Microscopy ◽  
Three Dimensional ◽  
Transmitted Light ◽  
Label Free ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Studies onTernidens deminutusRailliet & Henry, 1909 (Nematoda) I. External morphology

1973 ◽  
Vol 47 (2) ◽  
pp. 119-126 ◽  
Author(s):  
J. M. Goldsmid ◽  
N. F. Lyons
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Size Range ◽  
Three Dimensional ◽  
Transmitted Light ◽  
External Morphology ◽  
Morphological Studies ◽  
Transmitted Light Microscopy ◽  
Dimensional Picture ◽  
Scanning Electron

The present paper describes the size range ofTernidens deminutusfrom human and baboon hosts in Rhodesia and discusses the possible reasons for the differences noted.Using transmitted light and the scanning electron microscope, the external morphology ofT. deminutushas been re-studied and compared to investigations by other authors using transmitted light microscopy alone.The paper also illustrates the value of the scanning electron microscope in morphological studies in helminthology, especially when used in conjunction with the light microscope, to give an excellent three-dimensional picture of the species under investigation.It is intended to follow this work with further studies on the anatomy, histology, ultrastructure and histochemistry ofTernidens deminutus.

scholarly journals The lighthouse at the end of the chromosome*

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 1427 ◽  
Author(s):  
Yahya Benslimane ◽  
Lea Harrington
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Spatial Organization ◽  
Three Dimensional ◽  
Time Lapse ◽  
Living Cells ◽  
Telomere Elongation ◽  
Cellular Processes ◽  
Time Lapse Microscopy

Fluorescence microscopy can be used to assess the dynamic localization and intensity of single entities in vitro or in living cells. It has been applied with aplomb to many different cellular processes and has significantly enlightened our understanding of the heterogeneity and complexity of biological systems. Recently, high-resolution fluorescence microscopy has been brought to bear on telomeres, leading to new insights into telomere spatial organization and accessibility, and into the mechanistic nuances of telomere elongation. We provide a snapshot of some of these recent advances with a focus on mammalian systems, and show how three-dimensional, time-lapse microscopy and single-molecule fluorescence shine a new light on the end of the chromosome.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Three dimensional deblurring of transmitted-light brightfield micrographs

1993 ◽  
Vol 51 ◽  
pp. 564-565
Author(s):  
Santosh Bhattacharyya
Keyword(s):  
Image Reconstruction ◽  
Three Dimensional ◽  
Transmitted Light ◽  
Reconstruction Algorithms ◽  
3D Image Reconstruction ◽  
Structural Details ◽  
Spread Function ◽  
Early Testing ◽  
Linearized Model ◽  
Image Reconstruction Algorithms

Three dimensional microscopic structures play an important role in the understanding of various biological and physiological phenomena. Structural details of neurons, such as the density, caliber and volumes of dendrites, are important in understanding physiological and pathological functioning of nervous systems. Even so, many of the widely used stains in biology and neurophysiology are absorbing stains, such as horseradish peroxidase (HRP), and yet most of the iterative, constrained 3D optical image reconstruction research has concentrated on fluorescence microscopy. It is clear that iterative, constrained 3D image reconstruction methodologies are needed for transmitted light brightfield (TLB) imaging as well. One of the difficulties in doing so, in the past, has been in determining the point spread function of the system.We have been developing several variations of iterative, constrained image reconstruction algorithms for TLB imaging. Some of our early testing with one of them was reported previously. These algorithms are based on a linearized model of TLB imaging.

scholarly journals Nonparametric detection of changes over time in image data from fluorescence microscopy of living cells

2021 ◽  
Author(s):  
Kathrin Bissantz ◽  
Nicolai Bissantz ◽  
Katharina Proksch
Keyword(s):  
Fluorescence Microscopy ◽  
Image Data ◽  
Living Cells ◽  
Nonparametric Detection ◽  
Changes Over Time ◽  
Over Time

Visualizing three-dimensional fungal growth using light sheet fluorescence microscopy

2021 ◽  
pp. 103549
Author(s):  
Braulio Gutiérrez–Medina ◽  
Alexis Vázquez-Villa
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Fungal Growth ◽  
Light Sheet ◽  
Light Sheet Fluorescence Microscopy

Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

2011 ◽  
Vol 6 (11) ◽  
pp. 1748-1760 ◽  
Author(s):  
Ralf Schröder ◽  
Johannes Schmidt ◽  
Stefanie Blättermann ◽  
Lucas Peters ◽  
Nicole Janssen ◽  
...  
Keyword(s):  
G Protein ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Label Free ◽  
Dynamic Mass ◽  
Mass Redistribution ◽  
G Protein Coupled ◽  
Free Dynamic

scholarly journals High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity

2021 ◽  
Vol 118 (8) ◽  
pp. 081104
Author(s):  
Andrew J. Bower ◽  
Carlos Renteria ◽  
Joanne Li ◽  
Marina Marjanovic ◽  
Ronit Barkalifa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
High Speed ◽  
Label Free ◽  
Two Photon ◽  
Metabolic Transients ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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