scholarly journals Long-Term Culture of Stem Cells on Phosphate-Based Glass Microspheres: Synergistic Role of Chemical Formulation and 3D Architecture

2021 ◽  
Author(s):  
Dhanak Gupta ◽  
Kazi M. Zakir Hossain ◽  
Martin Roe ◽  
Emily F. Smith ◽  
Ifty Ahmed ◽  
...  
Keyword(s):  
Stem Cells ◽  
Glass Microspheres ◽  
Long Term Culture ◽  
3D Architecture

scholarly journals Long term culture and differentiation of endothelial progenitor like cells from rat adipose derived stem cells

2017 ◽  
Vol 70 (1) ◽  
pp. 397-413 ◽  
Author(s):  
Monire Amerion ◽  
Mojtaba Rezazadeh Valojerdi ◽  
Saeid Abroun ◽  
Mehdi Totonchi
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Endothelial Progenitor ◽  
Long Term Culture

scholarly journals DNA Methylation Changes duringIn VitroPropagation of Human Mesenchymal Stem Cells: Implications for Their Genomic Stability?

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Angela Bentivegna ◽  
Mariarosaria Miloso ◽  
Gabriele Riva ◽  
Dana Foudah ◽  
Valentina Butta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ex Vivo ◽  
Genomic Stability ◽  
Great Promise ◽  
Human Mscs ◽  
Low Oxygen ◽  
Long Term Culture ◽  
Functional Changes

Mesenchymal stem cells (MSCs) hold great promise for the treatment of numerous diseases. A major problem for MSC therapeutic use is represented by the very low amount of MSCs which can be isolated from different tissues; thusex vivoexpansion is indispensable. Long-term culture, however, is associated with extensive morphological and functional changes of MSCs. In addition, the concern that they may accumulate stochastic mutations which lead the risk of malignant transformation still remains. Overall, the genome of human MSCs (hMSCs) appears to be apparently stable throughout culture, though transient clonal aneuploidies have been detected. Particular attention should be given to the use of low-oxygen environment in order to increase the proliferative capacity of hMSCs, since data on the effect of hypoxic culture conditions on genomic stability are few and contradictory. Furthermore, specific and reproducible epigenetic changes were acquired by hMSCs duringex vivoexpansion, which may be connected and trigger all the biological changes observed. In this review we address current issues on long-term culture of hMSCs with a 360-degree view, starting from the genomic profiles and back, looking for an epigenetic interpretation of their genetic stability.

scholarly journals Enzymatic treatment of long-term human marrow cultures reveals the preferential location of primitive hemopoietic progenitors in the adherent layer

Blood ◽  
1983 ◽  
Vol 62 (2) ◽  
pp. 291-297 ◽  
Author(s):  
L Coulombel ◽  
AC Eaves ◽  
CJ Eaves
Keyword(s):  
Stem Cells ◽  
Marrow Cell ◽  
Point Of View ◽  
Adherent Cell ◽  
Reproducible Method ◽  
Preferential Location ◽  
Very High ◽  
Marrow Cultures

Abstract Recent studies with long-term mouse marrow cultures have indicated the importance of the adherent layer as a primary reservoir of the most primitive stem cells, from which derivative stem cells and more differentiated progenitors are continuously generated. We have now examined the role of the adherent cell layer in long-term human marrow cultures from this point of view. Prerequisite to such an undertaking was the development of a nontoxic and reproducible method for detaching the adherent layer and making it into a single-cell suspension suitable for characterization by colony assays. Both trypsin and collagenase could be used to obtain suspensions that met these criteria. Lack of toxicity was demonstrated by the preservation of CFU-E, BFU-E, and CFU- C plating efficiency in fresh human marrow cell suspensions exposed to the same enzymatic treatments. Collagenase treatment of long-term marrow culture adherent layers was considered superior because it freed all hemopoietic colony-forming cells but left some of the other cells still adherent. Using this method, we found that CFU-C, BFU-E, and CFU- G/E were consistently detectable in the adherent layer for at least 8 wk, with the majority of the BFU-E and CFU-G/E being located in the adherent layer (70%-75% after 2–3 wk and more than 90% by 7–8 wk). Although corresponding numerical differences in adherent and nonadherent CFU-C populations were not observed, the colonies derived from them showed marked differences in the size they achieved; the adherent layer being the exclusive site of CFU-C, with a very high proliferative capacity. These findings emphasize the importance of assessing the progenitor content of the adherent layer of long-term human marrow cultures and provide an appropriate methodology.

Long-Term Culture and Transplantation of Spermatogonial Stem Cells from BALB/c Mice

2010 ◽  
Vol 191 (5) ◽  
pp. 372-381 ◽  
Author(s):  
Fujin Shen ◽  
Ci Zhang ◽  
Hongyun Zheng ◽  
Yunhe Xiong ◽  
Xi Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Spermatogonial Stem Cells ◽  
Long Term Culture

scholarly journals A high-throughput polymer microarray approach for identifying defined substrates for mesenchymal stem cells

2014 ◽  
Vol 2 (11) ◽  
pp. 1683-1692 ◽  
Author(s):  
Cairnan R. E. Duffy ◽  
Rong Zhang ◽  
Siew-Eng How ◽  
Annamaria Lilienkampf ◽  
Guilhem Tourniaire ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
Polymer Substrates ◽  
Long Term Culture

Defined polymer substrates supported the growth of mesenchymal stem cells in long-term culture while maintaining their phenotype and lineage potential.

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