Fluorescent Molecular Sensor for the Detection of Cadmium in Basil Roots

2022 ◽  
Author(s):  
Yulieth C. Reyes R ◽  
Tahrima B. Rouf ◽  
Omar E. Torres ◽  
Edgar E. González
Keyword(s):  
Molecular Sensor

Quasi-planar B50 sheet as a potential molecular sensor for NO2: A DFT study

2021 ◽  
Vol 260 ◽  
pp. 124104
Author(s):  
Xiyuan Sun ◽  
Kaiming Wang ◽  
Pengfei Yin ◽  
Yi Zhang ◽  
Xing Feng
Keyword(s):  
Dft Study ◽  
Molecular Sensor

An Oxygen Molecular Sensor, the HIF Prolyl 4-Hydroxylase, in the Marine Protist Perkinsus olseni

Protist ◽  
2008 ◽  
Vol 159 (3) ◽  
pp. 355-368 ◽  
Author(s):  
Ricardo B. Leite ◽  
Anabela B. Brito ◽  
M.Leonor Cancela
Keyword(s):  
Molecular Sensor

scholarly journals A phosphoinositide-binding cluster in cavin1 acts as a molecular sensor for cavin1 degradation

2015 ◽  
Vol 26 (20) ◽  
pp. 3561-3569 ◽  
Author(s):  
Vikas A. Tillu ◽  
Oleksiy Kovtun ◽  
Kerrie-Ann McMahon ◽  
Brett M. Collins ◽  
Robert G. Parton
Keyword(s):  
Proteasomal Degradation ◽  
Resting Cells ◽  
Coat Proteins ◽  
Cellular Processes ◽  
Molecular Sensor ◽  
Lysine Residues ◽  
Low Levels ◽  
Membrane Stretching ◽  
Signaling Modules ◽  
Site Release

Caveolae are abundant surface organelles implicated in a range of cellular processes. Two classes of proteins work together to generate caveolae: integral membrane proteins termed caveolins and cytoplasmic coat proteins called cavins. Caveolae respond to membrane stress by releasing cavins into the cytosol. A crucial aspect of this model is tight regulation of cytosolic pools of cavin under resting conditions. We now show that a recently identified region of cavin1 that can bind phosphoinositide (PI) lipids is also a major site of ubiquitylation. Ubiquitylation of lysines within this site leads to rapid proteasomal degradation. In cells that lack caveolins and caveolae, cavin1 is cytosolic and rapidly degraded as compared with cells in which cavin1 is associated with caveolae. Membrane stretching causes caveolar disassembly, release of cavin complexes into the cytosol, and increased proteasomal degradation of wild-type cavin1 but not mutant cavin1 lacking the major ubiquitylation site. Release of cavin1 from caveolae thus leads to exposure of key lysine residues in the PI-binding region, acting as a trigger for cavin1 ubiquitylation and down-regulation. This mutually exclusive PI-binding/ubiquitylation mechanism may help maintain low levels of cytosolic cavin1 in resting cells, a prerequisite for cavins acting as signaling modules following release from caveolae.

A charge transfer-type fluorescent molecular sensor that “lights up” in the visible upon hydrogen bond-assisted complexation of anions

2004 ◽  
pp. 1946-1947 ◽  
Author(s):  
Anton Kovalchuk ◽  
Julia L. Bricks ◽  
Günter Reck ◽  
Knut Rurack ◽  
Burkhard Schulz ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Charge Transfer ◽  
Molecular Sensor ◽  
A Charge

scholarly journals SNAPSwitch: A Molecular Sensor to Quantify the Localization of Proteins, DNA and Nanoparticles in Cells

2019 ◽  
Author(s):  
Laura I FitzGerald ◽  
Luigi Aurelio ◽  
Moore Chen ◽  
Daniel Yuen ◽  
Bim Graham ◽  
...  
Keyword(s):  
Immune Responses ◽  
Intracellular Trafficking ◽  
Live Cells ◽  
Quantitative Detection ◽  
Dna Complexes ◽  
Receptor Signalling ◽  
Fluorescent Markers ◽  
Molecular Sensor ◽  
Protein Nucleic Acid ◽  
In Cells

Intracellular trafficking governs receptor signalling, pathogenesis, immune responses and the cellular fate of nanomedicines. These processes are typically tracked by confocal microscopy, where colocalization of fluorescent markers implies an interaction or co-compartmentalization. However, this type of analysis is inherently low-throughput, is limited by the resolution of microscopy, and can miss fleeting interactions. To address this, we have developed a localization sensor composed of a quenched and attachable SNAP-tag substrate (SNAPSwitch). SNAPSwitch enables quantitative detection of protein, nucleic acid and nanoparticle trafficking to locations of interest within live cells using flow cytometry. Using this approach, we followed the trafficking of DNA complexes travelling from endosomes into the cytosol and to the nucleus. We also show that antibody targeted to the transferrin (CD71) or hyaluronan (CD44) receptor is initially sorted into different compartments following endocytosis. These results demonstrate SNAPSwitch is a high-throughput and broadly applicable tool to quantitatively track the localization of materials in cells.

Micelle-Induced Versatile Sensing Behavior of Bispyrene-Based Fluorescent Molecular Sensor for Picric Acid and PYX Explosives

Langmuir ◽  
2014 ◽  
Vol 30 (26) ◽  
pp. 7645-7653 ◽  
Author(s):  
Liping Ding ◽  
Yumei Bai ◽  
Yuan Cao ◽  
Guijia Ren ◽  
Gary J. Blanchard ◽  
...  
Keyword(s):  
Picric Acid ◽  
Molecular Sensor

Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells

2012 ◽  
Vol 51 (17) ◽  
pp. 4148-4151 ◽  
Author(s):  
Toshitada Yoshihara ◽  
Yuji Yamaguchi ◽  
Masahiro Hosaka ◽  
Toshiyuki Takeuchi ◽  
Seiji Tobita
Keyword(s):  
Living Cells ◽  
Oxygen Levels ◽  
Molecular Sensor
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