Lipid Droplet-Specific Probe for Rapidly Locating Atherosclerotic Plaques and Intraoperative Imaging via In Situ Spraying

2021 ◽  
Author(s):  
Mangmang Sang ◽  
Bicheng Cai ◽  
Shuheng Qin ◽  
Shanshan Zhao ◽  
Yijie Mao ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Intraoperative Imaging ◽  
Atherosclerotic Plaques ◽  
Specific Probe

scholarly journals Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.

1991 ◽  
Vol 88 (18) ◽  
pp. 8154-8158 ◽  
Author(s):  
A. M. Henney ◽  
P. R. Wakeley ◽  
M. J. Davies ◽  
K. Foster ◽  
R. Hembry ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Atherosclerotic Plaques

Abstract 13: Inhibition of Nudt6 Stabilizes Advanced Atherosclerotic Plaques

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Hong Jin ◽  
Yuhuang Li ◽  
Ekaterina Chernogubova ◽  
Alexandra Bäcklund ◽  
Stina Sellberg ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Atherosclerotic Plaque ◽  
Plaque Rupture ◽  
Survival Rates ◽  
Atherosclerotic Plaques ◽  
Protein Coding ◽  
Fibrous Cap ◽  
Asymptomatic Patients ◽  
Rupture Model

Natural antisense transcripts (NATs), a non-coding RNA subclass, being transcribed in antisense direction to protein coding genes, are an intriguing novel class of targetable modulators, exerting crucial effects on gene expression. Aim of the current study was to investigate the contribution of NATs to atherosclerotic plaque vulnerability. Using laser capture micro-dissection, we isolated fibrous caps tissue of carotid artery plaques from 20 symptomatic patients with ruptured lesions vs. 20 samples from asymptomatic patients with stable lesions. A human transcriptome array (HTA; GeneChip 2.0 ) was used to profile the expression of all currently annotated RNA transcripts. Nucleoside diphosphate-linked moiety X motif 6 (NUDT6) was identified as one of the most significantly up-regulated transcripts in fibrous caps of ruptured lesions. Interestingly, NUDT6 is an established antisense RNA targeting the fibroblast growth factor 2 (FGF2). Of importance, FGF2 was among the most significantly down-regulated transcripts in ruptured lesions, corresponding to elevated NUDT6 expression. In situ hybridization in both, human and mouse carotid atherosclerotic plaques, confirmed substantially higher expression levels of NUDT6 in ruptured lesions compared to stable. In addition, in situ hybridization revealed a distinct co-localization with smooth muscle cells (SMCs) in advanced plaques. Overexpression of NUDT6 in cultured human carotid artery SMCs effectively limited FGF2 on the mRNA as well as protein level. Furthermore, reduction of NUDT6 via siRNA stimulated proliferation and blocked apoptosis in SMCs. In an inducible atherosclerotic plaque rupture model using incomplete ligation and cuff placement on common carotid arteries of male apoE -/- mice, NUDT6 inhibition with gapmeRs was able to significantly improve SMC survival rates, leading to thicker fibrous caps, and to reduce the plaque rupture rate compared to scramble-gapmeR control-treated mice (22% vs . 63%, p = 0.03). The present study presents NUDT6 as a novel crucial antisense regulator of fibrous cap stability through steering SMC survival via targeting its sense RNA transcript FGF2.

Detection of engraftment and chimerism after bone marrow transplantation by in situ hybridization using a Y-chromosome specific probe

1990 ◽  
Vol 33 (4) ◽  
pp. 255-260 ◽  
Author(s):  
Kazunaga Agematsu ◽  
Fuminori Kitahara ◽  
Yoshio Uehara ◽  
Hiroshi Kawai ◽  
Yukiaki Miyagawa ◽  
...  
Keyword(s):  
Bone Marrow ◽  
In Situ Hybridization ◽  
Bone Marrow Transplantation ◽  
Y Chromosome ◽  
Marrow Transplantation ◽  
Specific Probe

scholarly journals Detection, Isolation, and Characterization ofHelicobacterSpecies from the Gastrointestinal Tract of the Brushtail Possum

2011 ◽  
Vol 77 (5) ◽  
pp. 1581-1587 ◽  
Author(s):  
Thosaporn Coldham ◽  
Kerrie Rose ◽  
Jani O'rourke ◽  
Brett A. Neilan ◽  
Helen Dalton ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Brushtail Possum ◽  
Specific Probe ◽  
Lower Bowel ◽  
Eucalyptus Leaves ◽  
Isolation And Characterization ◽  
Australian Marsupial ◽  
Good Agreement

ABSTRACTThe presence ofHelicobacterspecies in Australian marsupials was examined systematically using microscopy, culture, and PCR in different regions of the gastrointestinal tract (GIT) and in the liver of brushtail possums (BTPs) (Trichosurus vulpecula), a common Australian marsupial that feeds on eucalyptus leaves. The spatial distribution ofHelicobacterspecies in the GIT sections also was examined microscopically in silver-stained sections and by fluorescentin situhybridization (FISH) using aHelicobactergenus-specific probe.Helicobacterspecies were found colonizing the lower bowel of all BTPs studied. Good agreement was observed between the detection ofHelicobacterspecies using culture and PCR, which was supported by the microscopic examination of silver-stained sections and FISH. The lower bowel of BTPs were colonized by one to three morphologically different (a comma-shaped species with no apparent flagella, a fusiform-shaped species entwined with periplasmic fibers and a bipolar sheathed flagella, and an S-shaped species with bipolar sheathed flagella) and potentially novelHelicobacterspecies, as well as in one case with a potentially novelCampylobacterspecies, which was a tightly coiled rod with bipolar unsheathed flagella. The isolation and characterization of theseHelicobacterspecies in BTPs provides important information regarding the specific natural niche of these bacteria and their corelationship within their host, and it increases our understanding of the ecology ofHelicobacterspecies.

Lipid‐Activatable Fluorescent Probe for Intraoperative Imaging of Atherosclerotic Plaque Using In Situ Patch

Small ◽  
2021 ◽  
pp. 2104471
Author(s):  
Jinrong Zheng ◽  
Shanshan Zhao ◽  
Yijie Mao ◽  
Zhichao Du ◽  
Gang Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Atherosclerotic Plaque ◽  
Intraoperative Imaging
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