Injectable Shear-Thinning CaSO4/FGF-18-Incorporated Chitin–PLGA Hydrogel Enhances Bone Regeneration in Mice Cranial Bone Defect Model

2017 ◽  
Vol 9 (49) ◽  
pp. 42639-42652 ◽  
Author(s):  
A. Sivashanmugam ◽  
Pornkawee Charoenlarp ◽  
S. Deepthi ◽  
Arunkumar Rajendran ◽  
Shantikumar V. Nair ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Shear Thinning ◽  
Cranial Bone ◽  
Defect Model

scholarly journals Evaluation of Guided Bone Regeneration Using the Bone Substitute Bio-Oss® and a Collagen Membrane in a Rat Cranial Bone Defect Model

2018 ◽  
Vol 27 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Shin Kasuya ◽  
Shihoko Inui ◽  
Nahoko Kato-Kogoe ◽  
Michi Omori ◽  
Kayoko Yamamoto ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Bone Substitute ◽  
Guided Bone Regeneration ◽  
Cranial Bone ◽  
Collagen Membrane ◽  
Defect Model

scholarly journals Periosteum progenitors could stimulate bone regeneration in aged murine bone defect model

2020 ◽  
Vol 24 (20) ◽  
pp. 12199-12210
Author(s):  
Han Xiao ◽  
Linfeng Wang ◽  
Tao Zhang ◽  
Can Chen ◽  
Huabin Chen ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Defect Model

Bone repair and augmentation using block of sintered bovine-derived anorganic bone graft in cranial bone defect model

2009 ◽  
Vol 20 (4) ◽  
pp. 340-350 ◽  
Author(s):  
Tania Mary Cestari ◽  
José Mauro Granjeiro ◽  
Gerson Francisco de Assis ◽  
Gustavo Pompermaier Garlet ◽  
Rumio Taga
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Bone Repair ◽  
Cranial Bone ◽  
Defect Model

scholarly journals Activation of Mesenchymal Stem Cells Promotes New Bone Formation Within Dentigerous Cyst

2020 ◽  
Author(s):  
Yejia Yu ◽  
Mengyu Li ◽  
Yuqiong Zhou ◽  
Yueqi Shi ◽  
Wenjie Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Dentigerous Cyst ◽  
Healing Process ◽  
Cell Counting ◽  
Cranial Bone ◽  
Alizarin Red

Abstract Background: Dentigerous cyst (DC) is a bone destructive disease and remains a challenge for clinicians. Marsupialization enables bone to regenerate with capsules maintaining, making it a preferred therapeutic means for DC adjacent to vital anatomical structures. Given that capsules of DC derive from odontogenic epithelium remnants at embryonic stage, we investigated whether there were mesenchymal stem cells (MSCs) located in DC capsules and the role that they played in the bone regeneration after marsupialization.Methods: Samples obtained before and after marsupialization were used for histological detection and cell culture. The stemness of cells isolated from fresh tissues were analyzed by morphology, surface marker and multi-differentiation assays. Comparison of proliferation ability between Am-DCSCs and Bm-DCSCs were evaluated by Cell Counting Kit-8 (CCK-8), fibroblast colony-forming units (CFU-F) and 5’‐ethynyl‐2’‐deoxyuridine (EdU) assay. Their osteogenic capacity in vitro was detected by Alkaline phosphatase (ALP) and Alizarin Red staining (ARS), combined with Real-time polymerase chain reaction (RT-PCR) and immunofluorescence (IF) staining. Subcutaneous ectopic osteogenesis as well as cranial bone defect model in nude mice were performed to detect their bone regeneration and bone defect repair ability.Results: Bone tissue and strong ALP activity were detected in the capsule of DC after marsupialization. Two types of MSCs were isolated from fibrous capsules of DC both before (Bm-DCSCs) and after (Am-DCSCs) marsupialization. These fibroblast-like, colony forming cells expressed MSC markers (CD44+, CD90+, CD31-, CD34-, CD45-), and they could differentiate into osteoblast-, adipocyte- and chondrocyte-like cells under induction. Notably, Am-DCSCs performed better in cell proliferation and self-renewal. Moreover, Am-DCSCs showed greater osteogenic capacity both in vitro and in vivo compared with Bm-DCSCs. Conclusions: There are MSCs residing in capsules of DC, and the cell viability as well as osteogenic capacity of them are largely enhanced after marsupialization. Our findings suggested that MSCs might play a crucial role in the healing process of DC after marsupialization, thus providing new insight into the treatment for DC by promoting the osteogenic differentiation of MSCs inside capsules.

scholarly journals The Development of Gelatin/Hyaluronate Copolymer Mixed with Calcium Sulfate, Hydroxyapatite, and Stromal-Cell-Derived Factor-1 for Bone Regeneration Enhancement

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1454 ◽  
Author(s):  
Yun-Liang Chang ◽  
Chia-Ying Hsieh ◽  
Chao-Yuan Yeh ◽  
Feng-Huei Lin
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Calcium Sulfate ◽  
Stromal Cell ◽  
Femoral Condyle ◽  
Bone Defects ◽  
Blood Analysis ◽  
Control Group ◽  
Defect Model ◽  
Stromal Cell Derived Factor

In clinical practice, bone defects still remain a challenge. In recent years, apart from the osteoconductivity that most bone void fillers already provide, osteoinductivity has also been emphasized to promote bone healing. Stromal-cell-derived factor-1 (SDF-1) has been shown to have the ability to recruit mesenchymal stem cells (MSCs), which play an important role in the bone regeneration process. In this study, we developed a gelatin–hyaluronate (Gel-HA) copolymer mixed with calcium sulfate (CS), hydroxyapatite (HAP), and SDF-1 in order to enhance bone regeneration in a bone defect model. The composites were tested in vitro for biocompatibility and their ability to recruit MSCs after material characterization. For the in vivo test, a rat femoral condyle bone defect model was used. Micro computed tomography (Micro-CT), two-photon excitation microscopy, and histology analysis were performed to assess bone regeneration. As expected, enhanced bone regeneration was well observed in the group filled with Gel-HA/CS/HAP/SDF-1 composites compared with the control group in our animal model. Furthermore, detailed blood analysis of rats showed no obvious systemic toxicity or side effects after material implantation. In conclusion, the Gel-HA/CS/HAP/SDF-1 composite may be a safe and applicable material to enhance bone regeneration in bone defects.

A new standardized critical size bone defect model in the pig forehead for comparative testing of bone regeneration materials

2019 ◽  
Vol 24 (5) ◽  
pp. 1651-1661
Author(s):  
Tobias Moest ◽  
Karl Andreas Schlegel ◽  
Marco Kesting ◽  
Matthias Fenner ◽  
Rainer Lutz ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Critical Size ◽  
Defect Model ◽  
Comparative Testing

scholarly journals Predifferentiated Gingival Stem Cell-Induced Bone Regeneration in Rat Alveolar Bone Defect Model

2020 ◽  
Author(s):  
Umadevi Kandalam ◽  
Toshihisa Kawai ◽  
Geeta Ravindran ◽  
Ross Brockman ◽  
Jorge Romero ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Alveolar Bone ◽  
Defect Model ◽  
Alveolar Bone Defect

scholarly journals Fabrication of Stromal Cell-Derived Factor-1 Contained in Gelatin/Hyaluronate Copo006Cymer Mixed with Hydroxyapatite for Use in Traumatic Bone Defects

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 822
Author(s):  
Yun-Liang Chang ◽  
Chia-Ying Hsieh ◽  
Chao-Yuan Yeh ◽  
Chih-Hao Chang ◽  
Feng-Huei Lin
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Stromal Cell ◽  
Second Harmonic ◽  
Bone Defects ◽  
In Vivo Studies ◽  
Control Group ◽  
Defect Model ◽  
Stromal Cell Derived Factor ◽  
Bone Void

Bone defects of orthopedic trauma remain a challenge in clinical practice. Regarding bone void fillers, besides the well-known osteoconductivity of most bone substitutes, osteoinductivity has also been gaining attention in recent years. It is known that stromal cell-derived factor-1 (SDF-1) can recruit mesenchymal stem cells (MSCs) in certain circumstances, which may also play an important role in bone regeneration. In this study, we fabricated a gelatin/hyaluronate (Gel/HA) copolymer mixed with hydroxyapatite (HAP) and SDF-1 to try and enhance bone regeneration in a bone defect model. After material characterization, these Gel/HA–HAP and Gel/HA–HAP–SDF-1 composites were tested for their biocompatibility and ability to recruit MSCs in vitro. A femoral condyle bone defect model of rats was used for in vivo studies. For the assessment of bone healing, micro-CT analysis, second harmonic generation (SHG) imaging, and histology studies were performed. As a result, the Gel/HA–HAP composites showed no systemic toxicity to rats. Gel/HA–HAP composite groups both showed better bone generation compared with the control group in an animal study, and the composite with the SDF-1 group even showed a trend of faster bone growth compared with the composite without SDF-1 group. In conclusion, in the management of traumatic bone defects, Gel/HA–HAP–SDF-1 composites can be a feasible material for use as bone void fillers.

scholarly journals Activation of mesenchymal stem cells promotes new bone formation within dentigerous cyst

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yejia Yu ◽  
Mengyu Li ◽  
Yuqiong Zhou ◽  
Yueqi Shi ◽  
Wenjie Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Dentigerous Cyst ◽  
Healing Process ◽  
Cell Counting ◽  
Cranial Bone ◽  
Alizarin Red

Abstract Background Dentigerous cyst (DC) is a bone destructive disease and remains a challenge for clinicians. Marsupialization enables the bone to regenerate with capsule maintaining, making it a preferred therapeutic means for DC adjacent to vital anatomical structures. Given that capsules of DC are derived from odontogenic epithelium remnants at the embryonic stage, we investigated whether there were mesenchymal stem cells (MSCs) located in DC capsules and the role that they played in the bone regeneration after marsupialization. Methods Samples obtained before and after marsupialization were used for histological detection and cell culture. The stemness of cells isolated from fresh tissues was analyzed by morphology, surface marker, and multi-differentiation assays. Comparison of proliferation ability between MSCs isolated from DC capsules before (Bm-DCSCs) and after (Am-DCSCs) marsupialization was evaluated by Cell Counting Kit-8 (CCK-8), fibroblast colony-forming units (CFU-F), and 5′-ethynyl-2′-deoxyuridine (EdU) assay. Their osteogenic capacity in vitro was detected by alkaline phosphatase (ALP) and Alizarin Red staining (ARS), combined with real-time polymerase chain reaction (RT-PCR) and immunofluorescence (IF) staining. Subcutaneous ectopic osteogenesis as well as cranial bone defect model in nude mice was performed to detect their bone regeneration and bone defect repairability. Results Bone tissue and strong ALP activity were detected in the capsule of DC after marsupialization. Two types of MSCs were isolated from fibrous capsules of DC both before (Bm-DCSCs) and after (Am-DCSCs) marsupialization. These fibroblast-like, colony-forming cells expressed MSC markers (CD44+, CD90+, CD31−, CD34−, CD45−), and they could differentiate into osteoblast-, adipocyte-, and chondrocyte-like cells under induction. Notably, Am-DCSCs performed better in cell proliferation and self-renewal. Moreover, Am-DCSCs showed a greater osteogenic capacity both in vitro and in vivo compared with Bm-DCSCs. Conclusions There are MSCs residing in capsules of DC, and the cell viability as well as the osteogenic capacity of them is largely enhanced after marsupialization. Our findings suggested that MSCs might play a crucial role in the healing process of DC after marsupialization, thus providing new insight into the treatment for DC by promoting the osteogenic differentiation of MSCs inside capsules.

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