Generating Microdroplet Array on Photonic Pseudo-paper for Absolute Quantification of Nucleic Acids

2018 ◽  
Vol 10 (45) ◽  
pp. 39144-39150 ◽  
Author(s):  
Junjie Chi ◽  
Changmin Shao ◽  
Xin Du ◽  
Hong Liu ◽  
Zhongze Gu
Keyword(s):  
Nucleic Acids ◽  
Absolute Quantification

scholarly journals Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA

Lab on a Chip ◽  
2015 ◽  
Vol 15 (13) ◽  
pp. 2759-2766 ◽  
Author(s):  
Friedrich Schuler ◽  
Frank Schwemmer ◽  
Martin Trotter ◽  
Simon Wadle ◽  
Roland Zengerle ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Absolute Quantification ◽  
Recombinase Polymerase Amplification ◽  
Dead Volume

Centrifugal step emulsification enables dead volume free emulsification, it was applied to the first digital droplet recombinase polymerase amplification (RPA).

Ultra-fast detection and quantification of nucleic acids by amplification-free fluorescence assay

The Analyst ◽  
2020 ◽  
Vol 145 (17) ◽  
pp. 5836-5844
Author(s):  
Jesper Uhd ◽  
Laura Miotke ◽  
Hanlee P. Ji ◽  
Marina Dunaeva ◽  
Ger J. M. Pruijn ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Absolute Quantification ◽  
Fluorescence Assay ◽  
Fast Detection ◽  
Dna And Rna ◽  
Detection And Quantification

Fast and reliable assay for amplification-free absolute quantification of DNA and RNA in biofluids.

scholarly journals Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153359 ◽  
Author(s):  
Zhao Li ◽  
Yong Liu ◽  
Qingquan Wei ◽  
Yuanjie Liu ◽  
Wenwen Liu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Absolute Quantification ◽  
Recombinase Polymerase Amplification

Clarity™ digital PCR system: a novel platform for absolute quantification of nucleic acids

2016 ◽  
Vol 409 (7) ◽  
pp. 1869-1875 ◽  
Author(s):  
Huiyu Low ◽  
Shun-Jie Chan ◽  
Guo-Hao Soo ◽  
Belinda Ling ◽  
Eng-Lee Tan
Keyword(s):  
Nucleic Acids ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
System A

Digital CRISPR-based Method for the Rapid Detection and Absolute Quantification of Nucleic Acids

Biomaterials ◽  
2021 ◽  
pp. 120876
Author(s):  
Xiaolin Wu ◽  
Joshua K. Tay ◽  
Chuan Keng Goh ◽  
Cheryl Chan ◽  
Yie Hou Lee ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Rapid Detection ◽  
Absolute Quantification

scholarly journals Digital PCR - Methods and Protocols

2019 ◽  
Vol 63 (4) ◽  
Author(s):  
Paolo Catarsi
Keyword(s):  
Nucleic Acids ◽  
Real Time ◽  
Quantitative Pcr ◽  
Dna Amplification ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
Relative Measurement ◽  
Real Time Monitoring ◽  
Major Step ◽  
Precision And Accuracy

Sensitivity, reproducibility, precision and accuracy are recurring words in this book. Indeed, digital PCR (dPCR) represents a major step forward in the quantification of nucleic acids. In this field, for many years it has been used the quantitative PCR (qPCR), a technology that allows a real-time monitoring of DNA amplification which has proved useful for obtaining a relative measurement, but that only indirectly, through the use of reference standards, provides an absolute quantification....

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