scholarly journals Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

2021 ◽  
Author(s):  
Philipp Werther ◽  
Klaus Yserentant ◽  
Felix Braun ◽  
Kristin Grußmayer ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Fluorogenic Probes ◽  
Super Resolution Microscopy

scholarly journals Bioorthogonal red and far-red fluorogenic probes for wash-free live-cell and super-resolution microscopy

2020 ◽  
Author(s):  
Philipp Werther ◽  
Klaus Yserentant ◽  
Felix Braun ◽  
Kristin Grussmayer ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
Unnatural Amino Acids ◽  
Super Resolution ◽  
Live Cell ◽  
Minimal Distance ◽  
Long Wavelength ◽  
Close Proximity ◽  
Fluorogenic Probes ◽  
Target Binding ◽  
Super Resolution Microscopy ◽  
Excited State Quenching

AbstractSmall-molecule fluorophores enable the observation of biomolecules in their native context with fluorescence microscopy. Specific labelling via bioorthogonal tetrazine chemistry confers minimal label size and rapid labelling kinetics. At the same time, fluorogenic tetrazine-dye conjugates exhibit efficient quenching of dyes prior to target binding. However, live-cell compatible long-wavelength fluorophores with strong fluorogenicity have been difficult to realize. Here, we report close proximity tetrazine-dye conjugates with minimal distance between tetrazine and fluorophore. Two synthetic routes give access to a series of cell permeable and impermeable dyes including highly fluorogenic far-red emitting derivatives with electron exchange as dominant excited state quenching mechanism. We demonstrate their potential for live-cell imaging in combination with unnatural amino acids, wash-free multi-colour and super-resolution STED and SOFI imaging. These dyes pave the way for advanced fluorescence imaging of biomolecules with minimal label size.

scholarly journals Ultrasmall, Bright, and Photostable Fluorescent Core–Shell Aluminosilicate Nanoparticles for Live‐Cell Optical Super‐Resolution Microscopy

2021 ◽  
Vol 33 (8) ◽  
pp. 2006829
Author(s):  
Jacob A. Erstling ◽  
Joshua A. Hinckley ◽  
Nirmalya Bag ◽  
Jessica Hersh ◽  
Grant B. Feuer ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Core Shell ◽  
Super Resolution Microscopy

scholarly journals Live cell single molecule-guided Bayesian localization super resolution microscopy

Cell Research ◽  
2016 ◽  
Vol 27 (5) ◽  
pp. 713-716 ◽  
Author(s):  
Fan Xu ◽  
Mingshu Zhang ◽  
Wenting He ◽  
Renmin Han ◽  
Fudong Xue ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Live Cell ◽  
Super Resolution Microscopy

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

2018 ◽  
Vol 141 (7) ◽  
pp. 2770-2781 ◽  
Author(s):  
Lu Wang ◽  
Michelle S. Frei ◽  
Aleksandar Salim ◽  
Kai Johnsson
Keyword(s):  
Small Molecule ◽  
Fluorescent Probes ◽  
Super Resolution ◽  
Live Cell ◽  
Super Resolution Microscopy

High-speed Live-cell Super-resolution Microscopy with Stochastically Switching Fluorophores

CLEO: 2013 ◽  
2013 ◽  
Author(s):  
Fang Huang ◽  
Tobias M. P. Hartwich ◽  
Felix E. Rivera-Molina ◽  
Yu Lin ◽  
Jordan R. Myers ◽  
...  
Keyword(s):  
High Speed ◽  
Super Resolution ◽  
Live Cell ◽  
Super Resolution Microscopy

scholarly journals Live cell single molecule-guided Bayesian localization super resolution microscopy

Cell Research ◽  
2016 ◽  
Author(s):  
Fan Xu ◽  
Mingshu Zhang ◽  
Wenting He ◽  
Renmin Han ◽  
Fudong Xue ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Live Cell ◽  
Super Resolution Microscopy

scholarly journals Small-Molecule Labeling of Live Cell Surfaces for Three-Dimensional Super-Resolution Microscopy

2014 ◽  
Vol 136 (40) ◽  
pp. 14003-14006 ◽  
Author(s):  
Marissa K. Lee ◽  
Prabin Rai ◽  
Jarrod Williams ◽  
Robert J. Twieg ◽  
W. E. Moerner
Keyword(s):  
Small Molecule ◽  
Three Dimensional ◽  
Super Resolution ◽  
Live Cell ◽  
Cell Surfaces ◽  
Super Resolution Microscopy
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