Transferring Microclusters of P. aeruginosa Biofilms to the Air–Liquid Interface of Bronchial Epithelial Cells for Repeated Deposition of Aerosolized Tobramycin

2021 ◽  
Author(s):  
Justus C. Horstmann ◽  
Annabelle Laric ◽  
Annette Boese ◽  
Daniela Yildiz ◽  
Teresa Röhrig ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Liquid Interface ◽  
Bronchial Epithelial ◽  
Air Liquid Interface

scholarly journals Growth and differentiation of primary and passaged equine bronchial epithelial cells under conventional and air-liquid-interface culture conditions

2011 ◽  
Vol 7 (1) ◽  
pp. 26 ◽  
Author(s):  
Getu Abraham ◽  
Claudia Zizzadoro ◽  
Johannes Kacza ◽  
Christin Ellenberger ◽  
Vanessa Abs ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Culture Conditions ◽  
Bronchial Epithelial Cells ◽  
Liquid Interface ◽  
Bronchial Epithelial ◽  
Air Liquid Interface

scholarly journals Role of Cigarette Smoke on Angiotensin-Converting Enzyme-2 Protein Membrane Expression in Bronchial Epithelial Cells Using an Air-Liquid Interface Model

2021 ◽  
Vol 12 ◽  
Author(s):  
Massimo Caruso ◽  
Alfio Distefano ◽  
Rosalia Emma ◽  
Michelino Di Rosa ◽  
Giuseppe Carota ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Selective Reduction ◽  
Bronchial Epithelial Cells ◽  
Liquid Interface ◽  
Membrane Expression ◽  
Bronchial Epithelial ◽  
Prevalence Studies ◽  
Positive Direct ◽  
Air Liquid Interface

Prevalence studies of current smoking, among hospitalized COVID-19 patients, demonstrated an unexpectedly low prevalence among patients with COVID-19. The aim of the present study was to evaluate the effect of smoke from cigarettes on ACE-2 in bronchial epithelial cells. Normal bronchial epithelial cells (H292) were exposed to smoke by an air-liquid-interface (ALI) system and ACE-2 membrane protein expression was evaluated after 24 h from exposure. Our transcriptomics data analysis showed a significant selective reduction of membrane ACE-2 expression (about 25%) following smoking exposure. Interestingly, we observed a positive direct correlation between ACE-2 reduction and nicotine delivery. Furthermore, by stratifying GSE52237 as a function of ACE-2 gene expression levels, we highlighted 1,012 genes related to ACE-2 in smokers and 855 in non-smokers. Furthermore, we showed that 161 genes involved in the endocytosis process were highlighted using the online pathway tool KEGG. Finally, 11 genes were in common between the ACE-2 pathway in smokers and the genes regulated during endocytosis, while 12 genes with non-smokers. Interestingly, six in non-smokers and four genes in smokers were closely involved during the viral internalization process. Our data may offer a pharmaceutical role of nicotine as potential treatment option in COVID-19.

scholarly journals Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air–Liquid Interface Cultured Human Primary Bronchial Epithelial Cells

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1335
Author(s):  
Bram M. ter Ellen ◽  
Nilima Dinesh Kumar ◽  
Ellen M. Bouma ◽  
Berit Troost ◽  
Denise P.I. van de Pol ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Antiviral Activity ◽  
Bronchial Epithelial Cells ◽  
Liquid Interface ◽  
Post Inoculation ◽  
Upper Respiratory Tract ◽  
African Green Monkey Kidney ◽  
Bronchial Epithelial ◽  
Air Liquid Interface ◽  
Primary Bronchial Epithelial Cells

The current COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has an enormous impact on human health and economy. In search for therapeutic options, researchers have proposed resveratrol, a food supplement with known antiviral, anti-inflammatory, and antioxidant properties as an advantageous antiviral therapy for SARS-CoV-2 infection. Here, we provide evidence that both resveratrol and its metabolically more stable structural analog, pterostilbene, exhibit potent antiviral properties against SARS-CoV-2 in vitro. First, we show that resveratrol and pterostilbene antiviral activity in African green monkey kidney cells. Both compounds actively inhibit virus replication within infected cells as reduced virus progeny production was observed when the compound was added at post-inoculation conditions. Without replenishment of the compound, antiviral activity was observed up to roughly five rounds of replication, demonstrating the long-lasting effect of these compounds. Second, as the upper respiratory tract represents the initial site of SARS-CoV-2 replication, we also assessed antiviral activity in air–liquid interface (ALI) cultured human primary bronchial epithelial cells, isolated from healthy volunteers. Resveratrol and pterostilbene showed a strong antiviral effect in these cells up to 48 h post-infection. Collectively, our data indicate that resveratrol and pterostilbene are promising antiviral compounds to inhibit SARS-CoV-2 infection. Because these results represent laboratory findings in cells, we advocate evaluation of these compounds in clinical trials before statements are made whether these drugs are advantageous for COVID-19 treatment.

scholarly journals Sub-ohm vaping increases the levels of carbonyls, is cytotoxic, and alters gene expression in human bronchial epithelial cells exposed at the air–liquid interface

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Alexandra Noël ◽  
Ekhtear Hossain ◽  
Zakia Perveen ◽  
Hasan Zaman ◽  
Arthur L. Penn
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Liquid Interface ◽  
Third Generation ◽  
Human Bronchial Epithelial Cells ◽  
Aerosol Composition ◽  
Exposure System ◽  
Bronchial Epithelial ◽  
Air Liquid Interface

Abstract Background Exposure to electronic-cigarette (e-cig) aerosols induces potentially fatal e-cig or vaping-associated lung injury (EVALI). The cellular and molecular mechanisms underlying these effects, however, are unknown. We used an air–liquid interface (ALI) in vitro model to determine the influence of two design characteristics of third-generation tank-style e-cig devices—resistance and voltage—on (1) e-cig aerosol composition and (2) cellular toxicity. Methods Human bronchial epithelial cells (H292) were exposed to either butter-flavored or cinnamon-flavored e-cig aerosols at the ALI in a Vitrocell exposure system connected to a third-generation e-cig device. Exposures were conducted following a standard vaping topography profile for 2 h per day, for 1 or 3 consecutive days. 24 h after ALI exposures cellular and molecular outcomes were assessed. Results We found that butter-flavored e-cig aerosol produced under ‘sub-ohm’ conditions (< 0.5 Ω) contains high levels of carbonyls (7–15 μg/puff), including formaldehyde, acetaldehyde and acrolein. E-cig aerosol produced under regular vaping conditions (resistance > 1 Ω and voltage > 4.5 V), contains lower carbonyl levels (< 2 μg/puff). We also found that the levels of carbonyls produced in the cinnamon-flavored e-cig aerosols were much lower than that of the butter-flavored aerosols. H292 cells exposed to butter-flavored or cinnamon-flavored e-cig aerosol at the ALI under ‘sub-ohm’ conditions for 1 or 3 days displayed significant cytotoxicity, decreased tight junction integrity, increased reactive oxygen species production, and dysregulated gene expression related to biotransformation, inflammation and oxidative stress (OS). Additionally, the cinnamon-flavored e-cig aerosol induced pro-oxidant effects as evidenced by increases in 8-hydroxy-2-deoxyguanosine protein levels. Moreover, we confirmed the involvement of OS as a toxicity process for cinnamon-flavored e-cig aerosol by pre-treating the cells with N-acetyl cysteine (NAC), an antioxidant that prevented the cells from the OS-mediated damage induced by the e-cig aerosol. Conclusion The production of high levels of carbonyls may be flavor specific. Overall, inhaling e-cig aerosols produced under ‘sub-ohm’ conditions is detrimental to lung epithelial cells, potentially via mechanisms associated with OS. This information could help policymakers take the necessary steps to prevent the manufacturing of sub-ohm atomizers for e-cig devices.

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