Plasmid DNA Undergoes Two Compaction Regimes under Macromolecular Crowding

2022 ◽  
pp. 186-192
Author(s):  
Suparna Khatun ◽  
Anurag Singh ◽  
Kumari Shikha ◽  
Agneyo Ganguly ◽  
Amar Nath Gupta
Keyword(s):  
Plasmid Dna ◽  
Macromolecular Crowding

Compaction of Plasmid DNA by Macromolecular Crowding

2017 ◽  
Vol 50 (4) ◽  
pp. 1666-1671 ◽  
Author(s):  
Amar Nath Gupta ◽  
Johan R. C. van der Maarel
Keyword(s):  
Plasmid Dna ◽  
Macromolecular Crowding

scholarly journals Synergistic interactions between confinement and macromolecular crowding spatially order transcription and translation in cell-free expression

2018 ◽  
Author(s):  
S. Elizabeth Norred ◽  
Rosemary M. Dabbs ◽  
Gaurav Chauhan ◽  
Patrick M. Caveney ◽  
C. Patrick Collier ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Spatial Organization ◽  
Macromolecular Crowding ◽  
Self Organization ◽  
Free Expression ◽  
Control Of Gene Expression ◽  
Free System ◽  
Synergistic Interactions ◽  
Transcriptional Dynamics ◽  
In Cells

ABSTRACTSynergistic interactions between macromolecular crowding and confinement spatially organize transcription and translation in cells. Yet, reproducing such spatial ordering in cell-free expression platforms has proven to be elusive. Here we report crowding- and confinement-driven spatial self-organization of cell-free expression that mimics expression behavior within and around the nucleoid of prokaryotes. These experiments use Ficoll-70 to approximate cellular macromolecular crowding conditions within cell-size lipid vesicles. Intriguingly, there was an abrupt change in transcriptional dynamics when crowding reached physiologically relevant levels. Imaging experiments revealed that this change in transcriptional dynamics was coincident with localization of plasmid DNA and mRNA at the vesicle wall. Computer simulations demonstrated that crowding leads to an entropically induced attraction between plasmid DNA and the wall, causing localization of DNA near the wall at sufficiently high crowding levels. The experiments demonstrate cell-like spatial organization of translation, where translational activity is controlled by chromosomally-templated positioning of mRNA. This cell-free system provides a flexible experimental platform to probe the underlying mechanisms of self-organization of membrane-less structures in cells and the spatial control of gene expression.

Polymeric Delivery of Proteins and Plasmid DNA for Tissue Engineering and Gene Therapy

2001 ◽  
Vol 11 (1-3) ◽  
pp. 12 ◽  
Author(s):  
Thomas P. Richardson ◽  
William L. Murphy ◽  
David J. Mooney
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Plasmid Dna ◽  
Polymeric Delivery

Evaluation of biocompatibility and efficiency of plasmid DNA delivery by gene-activated hydrogels in vitro

2019 ◽  
Vol XIV (2) ◽  
Author(s):  
I.Y. Bozo ◽  
A.A. Titova ◽  
M.N. Zhuravleva ◽  
A.I. Bilyalov ◽  
M.O. Mavlikeev ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Delivery

Detection of Rhizoctonia solani AG-2-2 IV, the Causal Agent of Large Patch of Zoysiagrass, Using Plasmid DNA as a Probe.

1998 ◽  
Vol 64 (5) ◽  
pp. 451-457 ◽  
Author(s):  
Susumu TAKAMATSU ◽  
Manami NAKANO ◽  
Hideyuki YOKOTA ◽  
Hitoshi KUNOH
Keyword(s):  
Rhizoctonia Solani ◽  
Plasmid Dna ◽  
Causal Agent ◽  
Large Patch
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