Polymeric Delivery of Proteins and Plasmid DNA for Tissue Engineering and Gene Therapy

2001 ◽  
Vol 11 (1-3) ◽  
pp. 12 ◽  
Author(s):  
Thomas P. Richardson ◽  
William L. Murphy ◽  
David J. Mooney
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Plasmid Dna ◽  
Polymeric Delivery

Muscle-Based Gene Therapy and Tissue Engineering

2001 ◽  
Vol 11 (1-3) ◽  
pp. 10 ◽  
Author(s):  
Dalip Pelinkovic ◽  
Joon Yung Lee ◽  
Nobuo Adachi ◽  
Johnny Huard
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy

Bone tissue engineering and repair by gene therapy

10.2741/2724 ◽  
2008 ◽  
Vol 13 (13) ◽  
pp. 833 ◽  
Author(s):  
Volker, M. Betz
Keyword(s):  
Tissue Engineering ◽  
Gene Therapy ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Arf6-mediated macropinocytosis enhanced suicide gene therapy of C16TAB-condensed Tat/pDNA nanoparticles in ovarian cancer

Nanoscale ◽  
2021 ◽  
Author(s):  
Zhe Sun ◽  
Jinhai Huang ◽  
Linjia Su ◽  
Jing Li ◽  
Fangzheng Qi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gene Therapy ◽  
Cancer Treatment ◽  
Plasmid Dna ◽  
Suicide Gene ◽  
Cell Penetrating Peptides ◽  
Therapeutic Gene ◽  
Hiv Tat ◽  
Efficient Delivery ◽  
Cell Penetrating

Using cell-penetrating peptides (CPPs), typically HIV-Tat, to deliver the therapeutic gene for cancer treatment has being hampered by low efficient delivery and complicated uptake route of plasmid DNA (pDNA). On...

Evaluation of Plasmid DNA for in Vivo Gene Therapy: Factors Affecting the Number of Transfected Fibers

1998 ◽  
Vol 87 (6) ◽  
pp. 763-768 ◽  
Author(s):  
Dominic J. Wells ◽  
Jake Maule ◽  
Jill McMahon ◽  
Roger Mitchell ◽  
Elsie Damien ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Factors Affecting

Mesenchymal Stem Cells for Bone Gene Therapy and Tissue Engineering

2002 ◽  
Vol 8 (21) ◽  
pp. 1917-1928 ◽  
Author(s):  
G. Pelled ◽  
G. Turgeman ◽  
H. Aslan ◽  
Z. Gazit ◽  
D. Gazit
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Gene Therapy ◽  
Mesenchymal Stem Cells

Gene Therapy and Tissue Engineering Based on Muscle-Derived Stem Cells

2007 ◽  
pp. 20-1-20-14
Author(s):  
Yong Li ◽  
Johnny Huard ◽  
Hairong Peng ◽  
Baohong Cao
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Gene Therapy

scholarly journals Improved downstream process for the production of plasmid DNA for gene therapy.

2005 ◽  
Vol 52 (3) ◽  
pp. 703-711 ◽  
Author(s):  
Jochen Urthaler ◽  
Wolfgang Buchinger ◽  
Roman Necina
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Viral Vectors ◽  
Continuous System ◽  
Size Exclusion ◽  
High Yield ◽  
Downstream Process ◽  
Naked Dna ◽  
Supercoiled Form ◽  
Exclusion Chromatography

Gene therapy and genetic vaccines promise to revolutionize the treatment of inherited and acquired diseases. Since viral vectors are generally associated with numerous disadvantages when applied to humans, the administration of naked DNA, or DNA packed into lipo- or polyplexes emerge as viable alternatives. To satisfy the increasing demand for pharmaceutical grade plasmids we developed a novel economic downstream process which overcomes the bottlenecks of common lab-scale techniques and meets all regulatory requirements. After cell lysis by an in-house developed gentle, automated continuous system the sequence of hydrophobic interaction, anion exchange and size exclusion chromatography guarantees the separation of impurities as well as undesired plasmid isoforms. After the consecutive chromatography steps, adjustment of concentration and final filtration are carried out. The final process was proven to be generally applicable and can be used from early clinical phases to market-supply. It is scaleable and free of animal-derived substances, detergents (except lysis) and organic solvents. The process delivers high-purity plasmid DNA of homogeneities up to 98% supercoiled form at a high yield in any desired final buffer.

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