scholarly journals Real-Time Respiration Changes as a Viability Indicator for Rapid Antibiotic Susceptibility Testing in a Microfluidic Chamber Array

ACS Sensors ◽  
2021 ◽  
Author(s):  
Petra Jusková ◽  
Steven Schmitt ◽  
André Kling ◽  
Darius G. Rackus ◽  
Martin Held ◽  
...  
Keyword(s):  
Real Time ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Microfluidic Chamber

scholarly journals Antibiotic Susceptibility Testing of Grown Blood Cultures by Combining Culture and Real-Time Polymerase Chain Reaction Is Rapid and Effective

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e27689 ◽  
Author(s):  
Judith Beuving ◽  
Annelies Verbon ◽  
Firza A. Gronthoud ◽  
Ellen E. Stobberingh ◽  
Petra F. G. Wolffs
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Blood Cultures ◽  
Antibiotic Susceptibility Testing ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Real-time respiration changes as a viability indicator for rapid antibiotic susceptibility testing in a microfluidic chamber array

2021 ◽  
Author(s):  
Petra Jusková ◽  
Steven Schmitt ◽  
André Kling ◽  
Darius G. Rackus ◽  
Martin Held ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Bacterial Infections ◽  
Susceptibility Testing ◽  
Pathogen Resistance ◽  
Rapid Identification ◽  
Individual Growth ◽  
Diagnostic Process ◽  
Antibiotic Susceptibility Testing ◽  
Microfluidic Chamber ◽  
Antibiotic Compounds

ABSTRACTRapid identification of a pathogen and the measurement of its antibiotic susceptibility are key elements in the diagnostic process of bacterial infections. Microfluidic technologies offer great control over handling and manipulation of low sample volumes with the possibility to study microbial cultures on the single-cell level. Downscaling the dimensions of cultivation systems directly results in a lower number of bacteria required for antibiotic susceptibility testing (AST) and thus in a reduction of the time to result. The developed platform presented in this work allows the reading of pathogen resistance profiles within 2-3 hours based on the changes of the dissolved oxygen levels during bacterial cultivation. The platform contains hundreds of individual growth chambers prefilled with a hydrogel containing oxygen-sensing nanoprobes and different concentrations of antibiotic compounds. The performance of the microfluidic platform is tested using quality control Escherichia coli strains (ATCC 25922 and ATCC 35218) in response to different clinically relevant antibiotics. The achieved results are in agreement with values given in clinical reference guides and independent measurements using a clinical AST protocol. Finally, the platform is successfully used for AST of an E. coli clinical isolate obtained from a patient blood culture.

scholarly journals Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0209042 ◽  
Author(s):  
Tucker Maxson ◽  
Candace D. Blancett ◽  
Amanda S. Graham ◽  
Christopher P. Stefan ◽  
Timothy D. Minogue
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Real Time ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Fast Antibiotic Susceptibility Testing via Raman Microspectrometry on Single Bacteria: An MRSA Case Study

ACS Omega ◽  
2021 ◽  
Author(s):  
Armelle Novelli Rousseau ◽  
Nicolas Faure ◽  
Fabian Rol ◽  
Zohreh Sedaghat ◽  
Joël Le Galudec ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Antibiotic Susceptibility Testing ◽  
Raman Microspectrometry

scholarly journals Longitudinal Characterization and Transmission Dynamics of Antibiotic-Resistant Organisms in an ICU (LOCATE AROs)

2020 ◽  
Vol 41 (S1) ◽  
pp. s42-s43
Author(s):  
Kimberley Sukhum ◽  
Candice Cass ◽  
Meghan Wallace ◽  
Caitlin Johnson ◽  
Steven Sax ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Environmental Samples ◽  
Susceptibility Testing ◽  
Marrow Transplant ◽  
Transmission Dynamics ◽  
Hospital Environment ◽  
Antibiotic Susceptibility Testing ◽  
Antibiotic Resistant ◽  
Clonal Group ◽  
Communal Areas

Background: Healthcare-associated infections caused by antibiotic-resistant organisms (AROs) are a major cause of significant morbidity and mortality. To create and optimize infection prevention strategies, it is crucial to delineate the role of the environment and clinical infections. Methods: Over a 14-month period, we collected environmental samples, patient feces, and patient bloodstream infection (BSI) isolates in a newly built bone marrow transplant (BMT) intensive care unit (ICU). Samples were collected from 13 high-touch areas in the patient room and 4 communal areas. Samples were collected from the old BMT ICU, in the new BMT ICU before patients moved in, and for 1 year after patients moved in. Selective microbiologic culture was used to isolate AROs, and whole-genome sequencing (WGS) was used to determine clonality. Antibiotic susceptibility testing was performed using Kirby-Bauer disk diffusion assays. Using linear mixed modeling, we compared ARO recovery across time and sample area. Results: AROs were collected and cultured from environmental samples, patient feces, and BSI isolates (Fig. 1a). AROs were found both before and after a patient entered the ICU (Fig. 1b). Sink drains had significantly more AROs recovered per sample than any other surface area (P < .001) (Fig. 1c). The most common ARO isolates were Pseudomonas aeruginosa and Stenotrophomonas maltophila (Fig. 1d). The new BMT ICU had fewer AROs recovered per sample than the old BMT ICU (P < .001) and no increase in AROs recovered over the first year of opening (P > .05). Furthermore, there was no difference before versus after patients moved into the hospital (P > .05). Antibiotic susceptibility testing reveal that P. aeruginosa isolates recovered from the old ICU were resistant to more antibiotics than isolates recovered from the new ICU (Fig. 2a). ANI and clonal analyses of P. aeruginosa revealed a large cluster of clonal isolates (34 of 76) (Fig. 2b). This clonal group included isolates found before patients moved into the BMT ICU and patient blood isolates. Furthermore, this clonal group was initially found in only 1 room in the BMT ICU, and over 26 weeks, it was found in sink drains in all 6 rooms sampled (Fig. 2b). Conclusions: AROs are present before patients move into a new BMT ICU, and sink drains act as a reservoir for AROs over time. Furthermore, sink-drain P. aeruginosa isolates are clonally related to isolates found in patient BSIs. Overall, these results provide insight into ARO transmission dynamics in the hospital environment.Funding: Research reported in this publication was supported by the Washington University Institute of Clinical and Translational Sciences grant UL1TR002345 from the National Center for Advancing Translational Sciences (NCATS) of the National Institutes of Health (NIH). The content is solely the responsibility of the authors and does not necessarily represent the official view of the NIH.Disclosures: None

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