An Ag nanoparticle included Glassy carbon (GC)/poly [3, 4-ethylenedioxythiophene] (PEDOT) modified electrode was organized by a straightforward electrochemical method without using any stabilizer or reducing agent. The obtained working electrodes showed a high conductivity when compared to the bare electrode. It also shows superior ability of electrochemically sensing towards the electroreduction of H2O2 with no need for an enzyme or mediator immobilized in the electrode. Under optimum condition the detection limit using chronoamperometry response was estimated to be 0.61 M based on the criterion of signal-to-noise ratio of 3 (S/N of 3).