AMD3100 Conjugates as Components of Targeted Nonviral Gene Delivery Systems:  Synthesis and in Vitro Transfection Efficiency of CXCR4-Expressing Cells

2004 ◽  
Vol 15 (2) ◽  
pp. 413-423 ◽  
Author(s):  
Bertrand Le Bon ◽  
Nathalie Van Craynest ◽  
Jean-Michel Daoudi ◽  
Christophe Di Giorgio ◽  
Abraham J. Domb ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Nonviral Gene Delivery ◽  
In Vitro Transfection

Octaarginine-modified chitosan as a nonviral gene delivery vector: properties and in vitro transfection efficiency

2010 ◽  
Vol 13 (2) ◽  
pp. 693-702 ◽  
Author(s):  
Xiaoli Zhao ◽  
Zhaoyang Li ◽  
Wenguang Liu ◽  
Wingmoon Lam ◽  
Peng Sun ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vector ◽  
Modified Chitosan ◽  
Delivery Vector ◽  
In Vitro Transfection

scholarly journals Exploitation of De Novo Helper-Lipids for Effective Gene Delivery.

2009 ◽  
Vol 11 (4) ◽  
pp. 56 ◽  
Author(s):  
Tomoaki Kurosaki ◽  
Takashi Kitahara ◽  
Mugen Teshima ◽  
Koyo Nishida ◽  
Junzo Nakamura ◽  
...  
Keyword(s):  
Gene Delivery ◽  
De Novo ◽  
Transfection Efficiency ◽  
The Other ◽  
Penetration Enhancers ◽  
Cell Toxicity ◽  
In Vivo Transfection ◽  
In Vitro Transfection

Purpose: In gene delivery, a fusogenic lipid such as dioleyl phosphatidylethanolamine (DOPE) which is a component of cationic liposomal vector is important factor for effective transfection efficiency. We investigated the effect of penetration enhancers as alternative helper-lipids to DOPE. Methods: Transdermal penetraion enhancers such as N-lauroylsarcosine (LS), (R)-(+)-limonene (LM), vitamin E (VE), and phosphatidyl choline from eggs (EggPC) were used in this experiments as helper-lipids with N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethlylammonium chloride (DOTMA) and cholesterol (CHOL). We examined in vitro transfection efficiency, cytotoxicity, hematotoxicity, and in vivo transfection efficiency of plasmid DNA/cationic liposomes complexes. Results: In transfection experiments in vitro, the cationic lipoplexes containing LS had highest transfection efficiency among the other lipoplexes independently of FBS. Furthermore, the lipoplexes containing LS had lowest cell toxicity among the other lipoplexes in the presence of FBS. As the results of erythrocytes interaction experiment, DOTMA/LS/CHOL, DOTMA/VE/CHOL, and DOTMA/EggPC/CHOL lipoplexes showed extremely lower hematotoxicity. On the basis of these results, the in vivo transfection efficiencies of the lipoplexes were examined. The lipoplexes containing LS had the highest transfection activity among the other lipoplexes. Conclusion: In conclusion, several transdermal penetration enhancers are available for alternative helper-lipids to DOPE in cationic liposomal vectors. Among them, DOTMA/LS/CHOL lipoplexes showed superior characteristics in in vitro transfection efficiency, cell toxicity, hematotoxicity, and in vivo transfection efficiency.

scholarly journals Formulation and in vitro transfection efficiency of poly (D, L-lactideco-glycolide) microspheres containing plasmid DNA for gene delivery

2000 ◽  
Vol 1 (4) ◽  
pp. 17-25 ◽  
Author(s):  
Sisay Gebrekidan ◽  
Byung H. Woo ◽  
Patrick P. DeLuca
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
In Vitro Transfection

Tunable nonviral gene delivery nanoparticles optimized for in vitro transfection of hepatocytes and hepatoma cells

2013 ◽  
Vol 24 (4) ◽  
pp. S91
Author(s):  
L.J. Higgins ◽  
S. Tzeng ◽  
M.G. Pomper ◽  
J. Green
Keyword(s):  
Gene Delivery ◽  
Hepatoma Cells ◽  
Nonviral Gene Delivery ◽  
In Vitro Transfection

Physical properties and in vitro transfection efficiency of gene delivery vectors based on complexes of DNA with synthetic polycations

2002 ◽  
Vol 81 (1-2) ◽  
pp. 201-217 ◽  
Author(s):  
Tomáš Reschel ◽  
Čestmı́r Koňák ◽  
David Oupický ◽  
Leonard W. Seymour ◽  
Karel Ulbrich
Keyword(s):  
Gene Delivery ◽  
Physical Properties ◽  
Transfection Efficiency ◽  
Gene Delivery Vectors ◽  
Synthetic Polycations ◽  
In Vitro Transfection

scholarly journals Highly Osmotic Oxidized Sucrose-Crosslinked Polyethylenimine for Gene Delivery Systems

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 87
Author(s):  
Jaehong Park ◽  
Kyusik Kim ◽  
Sohee Jeong ◽  
Migyeom Lee ◽  
Tae-il Kim
Keyword(s):  
Gene Delivery ◽  
Reductive Amination ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Buffering Capacity ◽  
Serum Stability ◽  
High Osmolality ◽  
Cox 2 ◽  
Serum Free Conditions ◽  
Positively Charged

In this work, highly osmotic oxidized sucrose-crosslinked polyethylenimine (SP2K) polymers were developed for gene delivery systems, and the transfection mechanism is examined. First, periodate-oxidized sucrose and polyethylenimine 2K (PEI2K) were crosslinked with various feed ratios via reductive amination. The synthesis was confirmed by 1H NMR and FTIR. The synthesized SP2K polymers could form positively charged (~40 mV zeta-potential) and nano-sized (150–200 nm) spherical polyplexes with plasmid DNA (pDNA). They showed lower cytotoxicity than PEI25K but concentration-dependent cytotoxicity. Among them, SP2K7 and SP2K10 showed higher transfection efficiency than PEI25K in both serum and serum-free conditions, revealing the good serum stability. It was found that SP2K polymers possessed high osmolality and endosome buffering capacity. The transfection experiments with cellular uptake inhibitors suggest that the transfection of SP2K polymers would progress by multiple pathways, including caveolae-mediated endocytosis. It was also thought that caveolae-mediated endocytosis of SP2K polyplexes would be facilitated through cyclooxygenase-2 (COX-2) expression induced by high osmotic pressure of SP2K polymers. Confocal microscopy results also supported that SP2K polyplexes would be internalized into cells via multiple pathways and escape endosomes efficiently via high osmolality and endosome buffering capacity. These results demonstrate the potential of SP2K polymers for gene delivery systems.

Effect of Polymer Ionization on the Interaction with DNA in Nonviral Gene Delivery Systems

2003 ◽  
Vol 4 (3) ◽  
pp. 683-690 ◽  
Author(s):  
Uracha Rungsardthong ◽  
Touraj Ehtezazi ◽  
Lindsey Bailey ◽  
Steven P. Armes ◽  
Martin C. Garnett ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery Systems ◽  
Nonviral Gene Delivery

Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation, Characterization and In Vitro Transfection

2015 ◽  
Vol 15 (7) ◽  
pp. 912-926 ◽  
Author(s):  
Ling Li ◽  
Fangfang Zhao ◽  
Baojing Zhao ◽  
Jin Zhang ◽  
Chao Li ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Macrocyclic Polyamine ◽  
Gene Delivery Vector ◽  
Delivery Vector ◽  
In Vitro Transfection
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