scholarly journals Formulation and in vitro transfection efficiency of poly (D, L-lactideco-glycolide) microspheres containing plasmid DNA for gene delivery

2000 ◽  
Vol 1 (4) ◽  
pp. 17-25 ◽  
Author(s):  
Sisay Gebrekidan ◽  
Byung H. Woo ◽  
Patrick P. DeLuca
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
In Vitro Transfection

scholarly journals Exploitation of De Novo Helper-Lipids for Effective Gene Delivery.

2009 ◽  
Vol 11 (4) ◽  
pp. 56 ◽  
Author(s):  
Tomoaki Kurosaki ◽  
Takashi Kitahara ◽  
Mugen Teshima ◽  
Koyo Nishida ◽  
Junzo Nakamura ◽  
...  
Keyword(s):  
Gene Delivery ◽  
De Novo ◽  
Transfection Efficiency ◽  
The Other ◽  
Penetration Enhancers ◽  
Cell Toxicity ◽  
In Vivo Transfection ◽  
In Vitro Transfection

Purpose: In gene delivery, a fusogenic lipid such as dioleyl phosphatidylethanolamine (DOPE) which is a component of cationic liposomal vector is important factor for effective transfection efficiency. We investigated the effect of penetration enhancers as alternative helper-lipids to DOPE. Methods: Transdermal penetraion enhancers such as N-lauroylsarcosine (LS), (R)-(+)-limonene (LM), vitamin E (VE), and phosphatidyl choline from eggs (EggPC) were used in this experiments as helper-lipids with N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethlylammonium chloride (DOTMA) and cholesterol (CHOL). We examined in vitro transfection efficiency, cytotoxicity, hematotoxicity, and in vivo transfection efficiency of plasmid DNA/cationic liposomes complexes. Results: In transfection experiments in vitro, the cationic lipoplexes containing LS had highest transfection efficiency among the other lipoplexes independently of FBS. Furthermore, the lipoplexes containing LS had lowest cell toxicity among the other lipoplexes in the presence of FBS. As the results of erythrocytes interaction experiment, DOTMA/LS/CHOL, DOTMA/VE/CHOL, and DOTMA/EggPC/CHOL lipoplexes showed extremely lower hematotoxicity. On the basis of these results, the in vivo transfection efficiencies of the lipoplexes were examined. The lipoplexes containing LS had the highest transfection activity among the other lipoplexes. Conclusion: In conclusion, several transdermal penetration enhancers are available for alternative helper-lipids to DOPE in cationic liposomal vectors. Among them, DOTMA/LS/CHOL lipoplexes showed superior characteristics in in vitro transfection efficiency, cell toxicity, hematotoxicity, and in vivo transfection efficiency.

scholarly journals In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Keiichi Motoyama ◽  
Yoshihiro Nakashima ◽  
Yukihiko Aramaki ◽  
Fumitoshi Hirayama ◽  
Kaneto Uekama ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Cationic Liposomes ◽  
Nonviral Vector ◽  
Liposomal Membrane ◽  
Transfer Activity ◽  
Potential Use

The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed the highest gene transfer activity in HepG2 cells without any significant cytotoxicity. In addition, γ-CyD enhanced the encapsulation ratio of pDNA with AF-liposomes, and also increased gene transfer activity as the entrapment ratio of pDNA into AF-liposomes was increased. γ-CyD stabilized the liposomal membrane of AF-liposomes and inhibited the release of calcein from AF-liposomes. The stabilizing effect of γ-CyD may be, at least in part, involved in the enhancing gene transfer activity of pDNA/γ-CyD/AF-liposomes. Therefore, these results suggest the potential use of γ-CyD for an enhancer of transfection efficiency of AF-liposomes.

AMD3100 Conjugates as Components of Targeted Nonviral Gene Delivery Systems:  Synthesis and in Vitro Transfection Efficiency of CXCR4-Expressing Cells

2004 ◽  
Vol 15 (2) ◽  
pp. 413-423 ◽  
Author(s):  
Bertrand Le Bon ◽  
Nathalie Van Craynest ◽  
Jean-Michel Daoudi ◽  
Christophe Di Giorgio ◽  
Abraham J. Domb ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Delivery Systems ◽  
Nonviral Gene Delivery ◽  
In Vitro Transfection

Octaarginine-modified chitosan as a nonviral gene delivery vector: properties and in vitro transfection efficiency

2010 ◽  
Vol 13 (2) ◽  
pp. 693-702 ◽  
Author(s):  
Xiaoli Zhao ◽  
Zhaoyang Li ◽  
Wenguang Liu ◽  
Wingmoon Lam ◽  
Peng Sun ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vector ◽  
Modified Chitosan ◽  
Delivery Vector ◽  
In Vitro Transfection

Physical properties and in vitro transfection efficiency of gene delivery vectors based on complexes of DNA with synthetic polycations

2002 ◽  
Vol 81 (1-2) ◽  
pp. 201-217 ◽  
Author(s):  
Tomáš Reschel ◽  
Čestmı́r Koňák ◽  
David Oupický ◽  
Leonard W. Seymour ◽  
Karel Ulbrich
Keyword(s):  
Gene Delivery ◽  
Physical Properties ◽  
Transfection Efficiency ◽  
Gene Delivery Vectors ◽  
Synthetic Polycations ◽  
In Vitro Transfection

Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation, Characterization and In Vitro Transfection

2015 ◽  
Vol 15 (7) ◽  
pp. 912-926 ◽  
Author(s):  
Ling Li ◽  
Fangfang Zhao ◽  
Baojing Zhao ◽  
Jin Zhang ◽  
Chao Li ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Macrocyclic Polyamine ◽  
Gene Delivery Vector ◽  
Delivery Vector ◽  
In Vitro Transfection

Evaluation of low molecular weight polyethylenimine-introduced chitosan for gene delivery to mesenchymal stem cells

2020 ◽  
Vol 10 (7) ◽  
pp. 1170-1176
Author(s):  
Minchen Liu ◽  
Yulan Hu ◽  
Yi Feng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
A549 Cells ◽  
Safety Evaluation ◽  
Physicochemical Characteristics ◽  
Hatching Rate

This study aimed to examine the transfection ability of polyethylenimine (PEI) (1800 Da)-grafted chitosan (10 kDa) (CP), a newly synthesized PEI derivative, in mesenchymal stem cells (MSCs). The safety evaluation of the complex/DNA was studied in vitro and in vivo. In addition, CP/pGL3 was applied to investigate the effects of transfection efficiency. In this study, CP/DNA can be formed with compatible physicochemical characteristics for gene delivery. CP cytotoxicity decreased in A549 cells. Moreover, a zebrafish embryo model was used for evaluating the safety in vivo. Compared to the PEI (25 kDa) group, the zebrafish hatching rate increased and the mortality rate decreased in the CP/DNA group, which provided an indication of the safety of CP. In comparison with chitosan (100 kDa)-PEI (1200 Da), CP's transfection efficiency was higher in both A549 cells and MSCs. This study aimed to lay the foundation for further applications of CP in gene delivery. Therefore, further gene therapy investigations of CP by using MSCs need to be performed.

scholarly journals The effects of lipoplex formulation variables on the protein corona and comparisons with in vitro transfection efficiency

2013 ◽  
Vol 171 (3) ◽  
pp. 261-268 ◽  
Author(s):  
Jamie L. Betker ◽  
Joe Gomez ◽  
Thomas J. Anchordoquy
Keyword(s):  
Transfection Efficiency ◽  
Protein Corona ◽  
In Vitro Transfection ◽  
Formulation Variables
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