Evidence That DNA Polymerase δ Isolated by Immunoaffinity Chromatography Exhibits High-Molecular Weight Characteristics and Is Associated with the KIAA0039 Protein and RPA†

Biochemistry ◽  
2000 ◽  
Vol 39 (24) ◽  
pp. 7245-7254 ◽  
Author(s):  
Jinyao Mo ◽  
Li Liu ◽  
Argentina Leon ◽  
Nayef Mazloum ◽  
Marietta Y. W. T. Lee
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Dna Polymerase ◽  
Immunoaffinity Chromatography ◽  
Dna Polymerase Δ ◽  
Molecular Weight Characteristics

Immunoaffinity Chromatography of Canine High-Molecular-Weight Renin: Partial Purification and Characterization

1983 ◽  
Vol 65 (2) ◽  
pp. 117-120 ◽  
Author(s):  
Fumihiko Ikemoto ◽  
Victor J. Dzau ◽  
Edgar Haber ◽  
Kazuo Takaori ◽  
Kenjiro Yamamoto
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Specific Activity ◽  
Immunoaffinity Chromatography ◽  
Partial Purification ◽  
Low Molecular Weight ◽  
Specific Method ◽  
Disulphide Bonds ◽  
Binding Substance ◽  
Molecular Weight Form

1. Canine high-molecular-weight renin (mol. wt. 60 000) is believed to be a complex of renin (low-molecular-weight form, mol. wt. 40 000) and renin-binding substance. The immunocross-reactivity of high-molecular-weight renin and low-molecular-weight renin was demonstrated by using antibodies specific to low-molecular-weight renin. 2. Immunoaffinity chromatography with renin-specific antibodies coupled to Sepharose provided a simple and specific method for isolation of high-molecular-weight renin. High-molecular-weight renin with a specific activity of 137 600 ng of ANG I h−1 mg−1 of protein (19.6 Goldblatt units/mg of protein) was obtained. 3. This high-molecular-weight renin was stable in dithiothreitol (25 mmol/l), suggesting that disulphide bonds may not be involved in the binding mechanism between low-molecular-weight renin and renin-binding substance. 4. However, exposure to low pH (3.0) resulted in conversion of high-molecular-weight renin into the low-molecular-weight form.

scholarly journals Evidence that a high molecular weight replicative DNA polymerase is conserved during evolution.

1981 ◽  
Vol 78 (11) ◽  
pp. 6771-6775 ◽  
Author(s):  
U. Hubscher ◽  
A. Spanos ◽  
W. Albert ◽  
F. Grummt ◽  
G. R. Banks
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Dna Polymerase

scholarly journals Cloning and characterization of the 5' part of the high molecular weight transcript of rat DNA polymerase beta.

1995 ◽  
Vol 42 (2) ◽  
pp. 253-258
Author(s):  
R Konopiński ◽  
R Nowak ◽  
J A Siedlecki
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Dna Polymerase ◽  
Alternative Polyadenylation ◽  
Dna Polymerase Beta ◽  
High Molecular Weight Dna ◽  
Reading Frame ◽  
Polymerase Beta ◽  
Brain Cdna Library ◽  
Specific Regulation

A rat brain cDNA library has been constructed. Three identical clones of about 2.2 kb representing high molecular weight DNA polymerase beta transcript were found. Sequencing proved our earlier suggestion that both high and low molecular weight DNA polymerase beta transcripts have the same open reading frame and differ mainly at the 3' end. Because of that, alternative polyadenylation is discussed as a possible mechanism for tissue- and development-specific regulation of the DNA polymerase beta gene expression.

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