human brain tumors
Recently Published Documents


TOTAL DOCUMENTS

534
(FIVE YEARS 22)

H-INDEX

63
(FIVE YEARS 2)

Data ◽  
2021 ◽  
Vol 6 (12) ◽  
pp. 132
Author(s):  
Denis S. Zavorotnyuk ◽  
Stanislav I. Pekov ◽  
Anatoly A. Sorokin ◽  
Denis S. Bormotov ◽  
Nikita Levin ◽  
...  

Alterations in cell metabolism, including changes in lipid composition occurring during malignancy, are well characterized for various tumor types. However, a significant part of studies that deal with brain tumors have been performed using cell cultures and animal models. Here, we present a dataset of 124 high-resolution negative ionization mode lipid profiles of human brain tumors resected during neurosurgery. The dataset is supplemented with 38 non-tumor pathological brain tissue samples resected during elective surgery. The change in lipid composition alterations of brain tumors enables the possibility of discriminating between malignant and healthy tissues with the implementation of ambient mass spectrometry. On the other hand, the collection of clinical samples allows the comparison of the metabolism alteration patterns in animal models or in vitro models with natural tumor samples ex vivo. The presented dataset is intended to be a data sample for bioinformaticians to test various data analysis techniques with ambient mass spectrometry profiles, or to be a source of clinically relevant data for lipidomic research in oncology.


2021 ◽  
Author(s):  
Wenjuan Zhang ◽  
Surabhi Talele ◽  
Jann N Sarkaria ◽  
William F Elmquist

Author(s):  
Elena Hernando-Pérez ◽  
Enrique Pérez-Riesgo ◽  
Santiago Cepeda ◽  
Ignacio Arrese ◽  
Rosario Sarabia ◽  
...  

Cancers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 960
Author(s):  
Halina Abramczyk ◽  
Beata Brozek-Pluska ◽  
Monika Kopec ◽  
Jakub Surmacki ◽  
Maciej Błaszczyk ◽  
...  

To monitor redox state changes and biological mechanisms occurring in mitochondrial cytochromes in cancers improving methods are required. We used Raman spectroscopy and Raman imaging to monitor changes in the redox state of the mitochondrial cytochromes in ex vivo human brain and breast tissues at 532 nm, 633 nm, 785 nm. We identified the oncogenic processes that characterize human infiltrating ductal carcinoma (IDC) and human brain tumors: gliomas; astrocytoma and medulloblastoma based on the quantification of cytochrome redox status by exploiting the resonance-enhancement effect of Raman scattering. We visualized localization of cytochromes by Raman imaging in the breast and brain tissues and analyzed cytochrome c vibrations at 750, 1126, 1337 and 1584 cm-1 as a function of malignancy grade. We found that the concentration of reduced cytochrome c becomes abnormally high in human brain tumors and breast cancers and correlates with the grade of cancer. We showed that Raman imaging provides additional insight into the biology of astrocytomas and breast ductal invasive cancer, which can be used for noninvasive grading, differential diagnosis.


2021 ◽  
Author(s):  
Johanna Schaffenrath ◽  
Tania Wyss ◽  
Liqun He ◽  
Elisabeth Jane Rushing ◽  
Mauro Delorenzi ◽  
...  

Abstract Background Brain tumors, whether primary or secondary, have limited therapeutic options despite advances in understanding driver gene mutations and heterogeneity within tumor cells. The cellular and molecular composition of brain tumor stroma, an important modifier of tumor growth, has been less investigated to date. Only few studies have focused on the vasculature of human brain tumors despite the fact that the blood-brain barrier (BBB) represents the major obstacle for efficient drug delivery. Methods In this study, we employed RNA sequencing to characterize transcriptional alterations of endothelial cells isolated from primary and secondary human brain tumors. We used an immunoprecipitation approach to enrich for endothelial cells from normal brain, glioblastoma (GBM) and lung cancer brain metastasis (BM). Results Analysis of the endothelial transcriptome showed deregulation of genes implicated in cell proliferation, angiogenesis and deposition of extracellular matrix (ECM) in the vasculature of GBM and BM. Deregulation of genes defining the BBB dysfunction module were found in both tumor types. We identified deregulated expression of genes in vessel-associated fibroblasts in GBM. Conclusion We characterize alterations in BBB genes in GBM and BM vasculature and identify proteins that might be exploited for developing drug delivery platforms. In addition, our analysis on vessel-associated fibroblasts in GBM shows that the cellular composition of brain tumor stroma merits further investigation.


Soft Matter ◽  
2021 ◽  
Author(s):  
Frank Sauer ◽  
Steffen Grosser ◽  
Steve Pawlizak ◽  
Tobias R. Kießling ◽  
Martin Reiss-Zimmermann ◽  
...  

Biomechanical changes are critical for cancer progression. However, the relationship between the rheology of single cells measured ex-vivo and the living tumor is not yet understood. Here, we combined single-cell...


2020 ◽  
Author(s):  
H. Abramczyk ◽  
B. Brozek-Pluska ◽  
M. Kopec ◽  
M. Błaszczyk ◽  
M. Radek

AbstractBackgroundTo monitoring redox state changes and biological mechanisms occurring in mitochondrial cytochromes in cancers improving novel methods are required.MethodsWe used Raman spectroscopy and Raman imaging to monitor changes in the redox state of the mitochondrial cytochromes in ex vivo human brain and breast tissues at 532 nm, 633 nm, 785 nm.ResultsWe identified the oncogenic processes that characterize human infiltrating ductal carcinoma (IDC) and human brain tumors: gliomas; astrocytoma and medulloblastoma based on the quantification of cytochrome redox status by exploiting the resonance-enhancement effect of Raman scattering. We visualized localization of cytochromes by Raman imaging in the breast and brain tissues and analyzed cytochrome c vibrations at 750, 1126, 1337 and 1584 cm-1 as a function of malignancy grade. We found that the concentration of reduced cytochrome c becomes abnormally high in human brain tumors and breast cancers and correlates with the grade of cancer aggressiveness.ConclusionsWe showed that Raman imaging provides additional insight into the biology of astrocytomas and breast ductal invasive cancer, which can be used for noninvasive grading, differential diagnosis, delineation of tumor extent, planning of surgery, and radiotherapy and post-treatment monitoring.Simple SummaryGliomas comprise around 30% of human brain tumors, while invasive ductal carcinoma (IDC) comprises around 80% of human breast cancers. The aim of our study was to show that cancerogenesis affects the redox status of mitochondrial cytochromes, which can be tracked by using Raman spectroscopy and imaging. We have shown the correlation between the intensity of cytochromes Raman bands at 750, 1126, 1337 and 1584 cm-1 and malignancy grade for brain and breast cancers.Graphical Abstract


2020 ◽  
Vol Volume 15 ◽  
pp. 7509-7521
Author(s):  
Mateusz Cieśluk ◽  
Katarzyna Pogoda ◽  
Piotr Deptuła ◽  
Paulina Werel ◽  
Alina Kułakowska ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document