Circular dichroism studies of barnase and its mutants: Characterization of the contribution of aromatic side chains

Biochemistry ◽  
1993 ◽  
Vol 32 (39) ◽  
pp. 10303-10313 ◽  
Author(s):  
Stephane Vuilleumier ◽  
Javier Sancho ◽  
Ron Loewenthal ◽  
Alan R. Fersht
1977 ◽  
Vol 81 (2) ◽  
pp. 395-401 ◽  
Author(s):  
Karl LINTNER ◽  
Serge FERMANDJIAN ◽  
Domenico REGOLI ◽  
Jean BARABE

Author(s):  
John P. Robinson ◽  
J. David Puett

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.


Biochemistry ◽  
1971 ◽  
Vol 10 (8) ◽  
pp. 1330-1335 ◽  
Author(s):  
C. Allen Bush ◽  
Stanley M. Ziegler

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