scholarly journals Transient Inactivation of Almond Mandelonitrile Lyase by 3-Methyleneoxindole: A Photooxidation Product of the Natural Plant Hormone Indole-3-Acetic Acid

Biochemistry ◽  
1994 ◽  
Vol 33 (10) ◽  
pp. 2891-2899 ◽  
Author(s):  
Ioanna P. Petrounia ◽  
Joel Goldberg ◽  
Edward J. Brush
Keyword(s):  
Acetic Acid ◽  
Plant Hormone ◽  
Natural Plant ◽  
Mandelonitrile Lyase ◽  
Indole 3 Acetic Acid

Structure Systematics of Auxin Herbicides. XXX. The Crystal Structures of (2-Isopropylphenoxy)-acetic Acid, (4-Fluorophenoxy)acetic Acid, (2,4-Dichloro-5-fluorophenoxy)acetic Acid and (Indol-3-ylthio)acetic Acid

1992 ◽  
Vol 45 (7) ◽  
pp. 1101 ◽  
Author(s):  
G Smith ◽  
DE Lynch ◽  
DS Sagatys ◽  
CHL Kennard ◽  
GF Katekar
Keyword(s):  
Acetic Acid ◽  
Crystal Structures ◽  
Plant Hormone ◽  
Side Chains ◽  
X Ray Diffraction ◽  
Natural Plant ◽  
Ring Systems ◽  
X Ray ◽  
Auxin Herbicides ◽  
Indole 3 Acetic Acid

The crystal structures of four analogues of the auxin herbicide series, including inactive examples, have been determined by X-ray diffraction methods. The compounds (2-isopropy1phenoxy)acetic acid (1), (4-fluorophenoxy)acetic acid (2) and (2,4-dichloro-5-fluorophenoxy)acetic acid (3) are from the phenoxyalkanoic acid series while (indol-3-ylthio)acetic acid (4) is the sulfur analogue of the natural plant hormone indole-3-acetic acid. All examples exist as hydrogen-bonded cyclic dimers [O…O, 2.640(7), 2.62(1), 2.637(4) and 2.665(4) � for (1)-(4), respectively]. Compounds (1) and (2) are essentially planar while compounds (3) and (4) have side chains anticlinally related to the ring systems, similar to (2,4-dich1orophenoxy)acetic acid and to indole-3-acetic acid.

scholarly journals Biochemical and Genetic Bases of Indole-3-Acetic Acid (Auxin Phytohormone) Degradation by the Plant-Growth-Promoting Rhizobacterium Paraburkholderia phytofirmans PsJN

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Raúl Donoso ◽  
Pablo Leiva-Novoa ◽  
Ana Zúñiga ◽  
Tania Timmermann ◽  
Gonzalo Recabarren-Gajardo ◽  
...  
Keyword(s):  
Energy Source ◽  
Gene Regulation ◽  
Acetic Acid ◽  
Plant Growth ◽  
Plant Hormone ◽  
Content Type ◽  
Gene Functions ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

ABSTRACT Several bacteria use the plant hormone indole-3-acetic acid (IAA) as a sole carbon and energy source. A cluster of genes (named iac) encoding IAA degradation has been reported in Pseudomonas putida 1290, but the functions of these genes are not completely understood. The plant-growth-promoting rhizobacterium Paraburkholderia phytofirmans PsJN harbors iac gene homologues in its genome, but with a different gene organization and context than those of P. putida 1290. The iac gene functions enable P. phytofirmans to use IAA as a sole carbon and energy source. Employing a heterologous expression system approach, P. phytofirmans iac genes with previously undescribed functions were associated with specific biochemical steps. In addition, two uncharacterized genes, previously unreported in P. putida and found to be related to major facilitator and tautomerase superfamilies, are involved in removal of an IAA metabolite called dioxindole-3-acetate. Similar to the case in strain 1290, IAA degradation proceeds through catechol as intermediate, which is subsequently degraded by ortho-ring cleavage. A putative two-component regulatory system and a LysR-type regulator, which apparently respond to IAA and dioxindole-3-acetate, respectively, are involved in iac gene regulation in P. phytofirmans. These results provide new insights about unknown gene functions and complex regulatory mechanisms in IAA bacterial catabolism. IMPORTANCE This study describes indole-3-acetic acid (auxin phytohormone) degradation in the well-known betaproteobacterium P. phytofirmans PsJN and comprises a complete description of genes, some of them with previously unreported functions, and the general basis of their gene regulation. This work contributes to the understanding of how beneficial bacteria interact with plants, helping them to grow and/or to resist environmental stresses, through a complex set of molecular signals, in this case through degradation of a highly relevant plant hormone.

Determination of Plant Hormone Indole-3-Acetic Acid in Aqueous Solution

2012 ◽  
Vol 25 (1) ◽  
pp. 303-307 ◽  
Author(s):  
Jana Bulíčková ◽  
Romana Sokolová ◽  
Stefania Giannarelli ◽  
Beatrice Muscatello
Keyword(s):  
Aqueous Solution ◽  
Acetic Acid ◽  
Plant Hormone ◽  
Indole 3 Acetic Acid

Identification of IAA-regulated genes in Pseudomonas syringae pv. tomato strain DC3000

2021 ◽  
Author(s):  
Arnaud-Thierry Djami-Tchatchou ◽  
Zipeng Alex Li ◽  
Paul Stodghill ◽  
Melanie J. Filiatrault ◽  
Barbara N. Kunkel
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Acetic Acid ◽  
Stress Response ◽  
Pseudomonas Syringae ◽  
Plant Pathogen ◽  
Type Iii Secretion ◽  
Plant Hormone ◽  
Indole 3 Acetic Acid ◽  
Exogenous Iaa

The auxin indole-3-acetic acid (IAA) is a plant hormone that not only regulates plant growth and development but also plays important roles in plant-microbe interactions. We previously reported that IAA alters expression of several virulence-related genes in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000 ( Pto DC3000). To learn more about the impact of IAA on regulation of Pto DC3000 gene expression we performed a global transcriptomic analysis of bacteria grown in culture, in the presence or absence of exogenous IAA. We observed that IAA repressed expression of genes involved in the Type III secretion (T3S) system and motility and promoted expression of several known and putative transcriptional regulators. Several of these regulators are orthologs of factors known to regulate stress responses and accordingly expression of several stress response-related genes was also upregulated by IAA. Similar trends in expression for several genes were also observed by RT-qPCR. Using an Arabidopsis thaliana auxin receptor mutant that accumulates elevated auxin, we found that many of the P. syringae genes regulated by IAA in vitro were also regulated by auxin in planta . Collectively the data indicate that IAA modulates many aspects of Pto DC3000 biology, presumably to promote both virulence and survival under stressful conditions, including those encountered in or on plant leaves. IMPORTANCE Indole-3-acetic acid (IAA), a form of the plant hormone auxin, is used by many plant-associated bacteria as a cue to sense the plant environment. Previously, we showed that IAA can promote disease in interactions between the plant pathogen Pseudomonas syringae strain Pto DC000 and one of its hosts, Arabidopsis thaliana . However, the mechanisms by which IAA impacts the biology of Pto DC3000 and promotes disease are not well understood. Here we demonstrate that IAA is a signal molecule that regulates gene expression in Pto DC3000. The presence of exogenous IAA affects expression of over 700 genes in the bacteria, including genes involved in Type III secretion and genes involved in stress response. This work offers insight into the roles of auxin promoting pathogenesis.

scholarly journals Evidence for an Increase in Microviscosity of Plasma Membranes from Soybean Hypocotyls Induced by the Plant Hormone, Indole-3-Acetic Acid

1976 ◽  
Vol 58 (4) ◽  
pp. 548-551 ◽  
Author(s):  
Sam L. Helgerson ◽  
William A. Cramer ◽  
D. James Morré
Keyword(s):  
Acetic Acid ◽  
Plasma Membranes ◽  
Plant Hormone ◽  
Indole 3 Acetic Acid

The Interaction of Human Serum with the Plant Hormone Indole-3-Acetic Acid

1976 ◽  
Vol 36 (1) ◽  
pp. 71-76
Author(s):  
JORGEN HILDEN ◽  
FOLKE RoNNIKE ◽  
HENNING SCHOU
Keyword(s):  
Acetic Acid ◽  
Human Serum ◽  
Plant Hormone ◽  
Indole 3 Acetic Acid

scholarly journals The main oxidative inactivation pathway of the plant hormone auxin

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ken-ichiro Hayashi ◽  
Kazushi Arai ◽  
Yuki Aoi ◽  
Yuka Tanaka ◽  
Hayao Hira ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Amino Acid ◽  
Plant Development ◽  
Plant Hormone ◽  
Amino Acid Conjugates ◽  
Auxin Homeostasis ◽  
Oxidative Inactivation ◽  
Indole 3 Acetic Acid

AbstractInactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated in auxin inactivation. In this study, we show that the predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via the GH3-ILR1-DAO pathway. IAA is first converted to IAA-amino acid conjugates by GH3 IAA-amidosynthetases. The IAA-amino acid conjugates IAA-aspartate (IAA-Asp) and IAA-glutamate (IAA-Glu) are storage forms of IAA and can be converted back to IAA by ILR1/ILL amidohydrolases. We further show that DAO1 dioxygenase irreversibly oxidizes IAA-Asp and IAA-Glu into 2-oxindole-3-acetic acid-aspartate (oxIAA-Asp) and oxIAA-Glu, which are subsequently hydrolyzed by ILR1 to release inactive oxIAA. This work established a complete pathway for the oxidative inactivation of auxin and defines the roles played by auxin homeostasis in plant development.

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