A specific cell surface glycoconjugate controlling cell motility: evidence by functional monoclonal antibodies that inhibit cell motility and tumor cell metastasis

Anti-Mac-1 (M1/70), a rat monoclonal antibody that reacts with mouse and human macrophages, polymorphonuclear leukocytes (PMNL), and natural killer cells, selectively inhibited complement receptor-mediated rosetting by murine macrophages and human PMNL. Preincubation of macrophages with anti-Mac-1 inhibited formation of rosettes with sheep erythrocytes bearing IgM antibody and murine C3 fragments. No inhibition was observed when other monoclonal antibodies that react with macrophages (such as anti-Ly5, anti-H-2, or anti-pan-leukocyte) were tested at 10-fold higher concentrations. Anti-Mac-1 did not affect macrophage Fc receptor-mediated rosetting. Erythrocytes bearing homogeneous human C3 fragments C3b (EC3b) or C3bi (EC3bi) were used to test the specificity of the murine macrophage and human PMNL complement receptor inhibited by anti-Mac-1. In both cases, anti-Mac-1 inhibited CR3-mediated rosetting of EC3bi but not CR1-dependent rosetting of EC3b. The results show that Mac-1 is either identical to CR3 or closely associated with CR3 function. This is one of the first cases in which a monoclonal antibody-defined differentiation antigen has been associated with a specific cell surface function.

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Specific cell surface labels in the visual centers of Xenopus laevis tadpole identified using monoclonal antibodies

Developmental Biology ◽

10.1016/0012-1606(87)90335-6 ◽

1987 ◽

Vol 122 (1) ◽

pp. 90-100 ◽

Cited By ~ 106

Author(s):

Shin Takagi ◽

Toshiaki Tsuji ◽

Takashi Amagai ◽

Tetsuro Takamatsu ◽

Hajime Fujisawa

Keyword(s):

Monoclonal Antibodies ◽

Cell Surface ◽

Xenopus Laevis ◽

Specific Cell ◽

Specific Cell Surface ◽

Xenopus Laevis Tadpole

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The expression of myeloid-specific antigens on myeloid leukemia cells: correlations with leukemia subclasses and implications for normal myeloid differentiation

Blood ◽

10.1182/blood.v61.3.456.bloodjournal613456 ◽

1983 ◽

Vol 61 (3) ◽

pp. 456-463

Author(s):

ED Ball ◽

MW Fanger

Keyword(s):

Monoclonal Antibodies ◽

Cell Surface ◽

Myeloid Leukemia ◽

Present Report ◽

Leukemia Cells ◽

Acute Myelocytic Leukemia ◽

Specific Cell ◽

Normal Peripheral Blood ◽

Myelocytic Leukemia ◽

Specific Cell Surface

The expression of three distinct myeloid-specific cell surface antigens detected by monoclonal antibodies (PMN 6, PMN 29, and AML-2–23) on acute and chronic myeloid leukemia cells is correlated with blast cell morphology and normal myeloid cell antigen display. In studies on normal peripheral blood cells, monoclonal antibodies PMN 6 and PMN 29 have previously been shown to react exclusively with neutrophils while AML-2–23 reacts with both neutrophils and monocytes. The present report demonstrates that these antigens are absent from blast cells of patients with acute myelocytic leukemia (AML) classified as M1 and M2 in the French-American-British system and chronic myelocytic leukemia in myeloid blast crisis. However, leukemia cells with myelomonocytic morphology (M4) expressed all three antigens, while cells with pure monocytic features (M5) were generally only positive for AML-2–23. Based on the absence of these antigens on both leukemic and normal myeloblasts and granulocyte-monocyte progenitors and their characteristic patterns of display on more differentiated leukemic and normal cells, we propose a modified concept of normal myelopoiesis. In this hypothesis, the myeloblast is an uncommitted cell that gives rise to a series of intermediate precursors that acquire committment to either the granulocytic or monocytic lineage marked by the acquisition of specific cell surface markers.

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Monoclonal antibodies against Neisseria gonorrhoeae: production of antibodies directed against a strain-specific cell surface antigen.

Infection and Immunity ◽

10.1128/iai.32.2.641-648.1981 ◽

1981 ◽

Vol 32 (2) ◽

pp. 641-648 ◽

Cited By ~ 24

Author(s):

I Nachamkin ◽

J G Cannon ◽

R S Mittler

Keyword(s):

Monoclonal Antibodies ◽

Cell Surface ◽

Neisseria Gonorrhoeae ◽

Surface Antigen ◽

Cell Surface Antigen ◽

Specific Cell ◽

Specific Cell Surface

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The expression of myeloid-specific antigens on myeloid leukemia cells: correlations with leukemia subclasses and implications for normal myeloid differentiation

Blood ◽

10.1182/blood.v61.3.456.456 ◽

1983 ◽

Vol 61 (3) ◽

pp. 456-463 ◽

Cited By ~ 63

Author(s):

ED Ball ◽

MW Fanger

Keyword(s):

Monoclonal Antibodies ◽

Cell Surface ◽

Myeloid Leukemia ◽

Present Report ◽

Leukemia Cells ◽

Acute Myelocytic Leukemia ◽

Specific Cell ◽

Normal Peripheral Blood ◽

Myelocytic Leukemia ◽

Specific Cell Surface

Abstract The expression of three distinct myeloid-specific cell surface antigens detected by monoclonal antibodies (PMN 6, PMN 29, and AML-2–23) on acute and chronic myeloid leukemia cells is correlated with blast cell morphology and normal myeloid cell antigen display. In studies on normal peripheral blood cells, monoclonal antibodies PMN 6 and PMN 29 have previously been shown to react exclusively with neutrophils while AML-2–23 reacts with both neutrophils and monocytes. The present report demonstrates that these antigens are absent from blast cells of patients with acute myelocytic leukemia (AML) classified as M1 and M2 in the French-American-British system and chronic myelocytic leukemia in myeloid blast crisis. However, leukemia cells with myelomonocytic morphology (M4) expressed all three antigens, while cells with pure monocytic features (M5) were generally only positive for AML-2–23. Based on the absence of these antigens on both leukemic and normal myeloblasts and granulocyte-monocyte progenitors and their characteristic patterns of display on more differentiated leukemic and normal cells, we propose a modified concept of normal myelopoiesis. In this hypothesis, the myeloblast is an uncommitted cell that gives rise to a series of intermediate precursors that acquire committment to either the granulocytic or monocytic lineage marked by the acquisition of specific cell surface markers.

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