Velocity of the creatine kinase reaction in the neonatal rabbit heart: role of mitochondrial creatine kinase

Biochemistry ◽  
1988 ◽  
Vol 27 (6) ◽  
pp. 2165-2172 ◽  
Author(s):  
Stanton B. Perry ◽  
John McAuliffe ◽  
James A. Balschi ◽  
Paul R. Hickey ◽  
Joanne S. Ingwall
Keyword(s):  
Creatine Kinase ◽  
Rabbit Heart ◽  
Mitochondrial Creatine Kinase ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction

Kinetics of the creatine kinase reaction in neonatal rabbit heart: an empirical analysis of the rate equation

Biochemistry ◽  
1991 ◽  
Vol 30 (10) ◽  
pp. 2585-2593 ◽  
Author(s):  
John J. McAuliffe ◽  
Stanton B. Perry ◽  
Elwood E. Brooks ◽  
Joanne S. Ingwall
Keyword(s):  
Creatine Kinase ◽  
Empirical Analysis ◽  
Rate Equation ◽  
Rabbit Heart ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction ◽  
Kinetics Of

Phosphocreatine pathway for energy transport: ADP diffusion and cardiomyopathy

1991 ◽  
Vol 261 (4) ◽  
pp. L30-L38
Author(s):  
V. A. Saks ◽  
Y. O. Belikova ◽  
A. V. Kuznetsov ◽  
Z. A. Khuchua ◽  
T. H. Branishte ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Energy Transport ◽  
Isolated Heart ◽  
Cellular Respiration ◽  
Regulation Of Respiration ◽  
Mitochondrial Creatine Kinase ◽  
Kinase Reaction ◽  
Cardiomyopathic Hamsters ◽  
Creatine Kinase Reaction ◽  
Cardiomyopathic Heart

Chemically skinned (by treatment with saponin, 40 μg/ml) isolated cardiomyocytes were used to study the intracellular diffusion of ADP and creatine (Cr). Stimulation of respiration was studied in these cardiomyocytes without intact sarcolemma and in isolated heart mitochondrial by addition of ADP and Cr in the presence of 0.2 mM ATP (via mitochondrial creatine kinase reaction: Cr + MgATP = MgADP + PCr). The Michaelis constant (Km) for Cr was similar in both cases, 5.67 ± 0.11 (SD) mM in skinned myocytes and 6.9 ± 0.2 mM in mitochondria, showing that there is no significant restriction to the diffusion of this substrate. However, the apparent Km for external ADP increased from 17.6 ± 1.0 μM for mitochondria to 250 ± 38 μM for skinned cardiomyocytes, showing decreased diffusivity of ADP as a result of binding to cellular structures. In the presence of 25 mM Cr, the Km for ADP for myocytes decreased to 35.6 ± 5.6 μM due to the coupling of the creatine kinase and oxidative phosphorylation reactions. Provision of substrate for the creatine kinase reaction amplified the weak ADP signal in the regulation of respiration. The activity of the mitochondrial creatine kinase was decreased by a factor of two in cardiomyopathic hamsters and human hearts and was associated with a twofold decrease in creatine-stimulated respiration. These data show a potentially key role of mitochondrial creatine kinase in the regulation of cellular respiration and the possible importance of changes in its activity for the functional disturbances of the cardiomyopathic heart. cellular respiration; creatine kinase; oxidative phosphorylati

Phosphocreatine pathway for energy transport: ADP diffusion and cardiomyopathy

1991 ◽  
Vol 261 (4) ◽  
pp. 30-38
Author(s):  
V. A. Saks ◽  
Y. O. Belikova ◽  
A. V. Kuznetsov ◽  
Z. A. Khuchua ◽  
T. H. Branishte ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Oxidative Phosphorylation ◽  
Energy Transport ◽  
Isolated Heart ◽  
Cellular Respiration ◽  
Regulation Of Respiration ◽  
Mitochondrial Creatine Kinase ◽  
Kinase Reaction ◽  
Cardiomyopathic Hamsters ◽  
Creatine Kinase Reaction

Chemically skinned (by treatment with saponin, 40 μg/ml) isolated cardiomyocytes were used to study the intracellular diffusion of ADP and creatine (Cr). Stimulation of respiration was studied in these cardiomyocytes without intact sarcolemma and in isolated heart mitochondrial by addition of ADP and Cr in the presence of 0.2 mM ATP (via mitochondrial creatine kinase reaction: Cr + MgATP = MgADP + PCr). The Michaelis constant (Km) for Cr was similar in both cases, 5.67 ± 0.11 (SD) mM in skinned myocytes and 6.9 ± 0.2 mM in mitochondria, showing that there is no significant restriction to the diffusion of this substrate. However, the apparent Km for external ADP increased from 17.6 ± 1.0 μM for mitochondria to 250 ± 38 μM for skinned cardiomyocytes, showing decreased diffusivity of ADP as a result of binding to cellular structures. In the presence of 25 mM Cr, the Km for ADP for myocytes decreased to 35.6 ± 5.6 μM due to the coupling of the creatine kinase and oxidative phosphorylation reactions. Provision of substrate for the creatine kinase reaction amplified the weak ADP signal in the regulation of respiration. The activity of the mitochondrial creatine kinase was decreased by a factor of two in cardiomyopathic hamsters and human hearts and was associated with a twofold decrease in creatine-stimulated respiration. These data show a potentially key role of mitochondrial creatine kinase in the regulation of cellular respiration and the possible importance of changes in its activity for the functional disturbances of the cardiomyopathic heart. cellular respiration; creatine kinase; oxidative phosphorylation

scholarly journals P3331Obesity is associated with an increase in the forward rate constant of the creatine kinase reaction

2017 ◽  
Vol 38 (suppl_1) ◽  
Author(s):  
J. Rayner ◽  
W. Clarke ◽  
M.A. Peterzan ◽  
C.T. Rodgers ◽  
S. Neubauer ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Rate Constant ◽  
Forward Rate ◽  
Kinase Reaction ◽  
Forward Rate Constant ◽  
Creatine Kinase Reaction

31P imaging of in Vivo creatine kinase reaction rates

1987 ◽  
Vol 74 (3) ◽  
pp. 574-579 ◽  
Author(s):  
Paul S Hsieh ◽  
Robert S Balaban
Keyword(s):  
Creatine Kinase ◽  
Reaction Rates ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction

scholarly journals Triple repetition time saturation transfer (TRiST) 31 P spectroscopy for measuring human creatine kinase reaction kinetics

2010 ◽  
Vol 63 (6) ◽  
pp. 1493-1501 ◽  
Author(s):  
Michael Schär ◽  
AbdEl-Monem M. El-Sharkawy ◽  
Robert G. Weiss ◽  
Paul A. Bottomley
Keyword(s):  
Creatine Kinase ◽  
Reaction Kinetics ◽  
Saturation Transfer ◽  
Kinase Reaction ◽  
Repetition Time ◽  
Creatine Kinase Reaction

A study of creatine kinase reaction in rat brain under chronic pathological conditions—chronic ischemia and ethanol intoxication

2000 ◽  
Vol 53 (4) ◽  
pp. 431-435 ◽  
Author(s):  
Svatava Kašparová ◽  
Dušan Dobrota ◽  
Vladimı́r Mlynárik ◽  
Tran N. Pham ◽  
Tibor Liptaj ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Rat Brain ◽  
Chronic Ischemia ◽  
Ethanol Intoxication ◽  
Kinase Reaction ◽  
Pathological Conditions ◽  
Creatine Kinase Reaction

scholarly journals A Flow-Quench Apparatus for Cryoenzymic Studies. Application to the Creatine Kinase Reaction

1980 ◽  
Vol 110 (2) ◽  
pp. 397-403 ◽  
Author(s):  
Thomas E. BARMAN ◽  
Andre BRUN ◽  
Franck TRAVERS
Keyword(s):  
Creatine Kinase ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction
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