Preparative polyacrylamide gel electrophoresis of ribonucleic acid. Identification of multiple molecular species of bacteriophage T7 lysozyme messenger ribonucleic acid

Biochemistry ◽  
1974 ◽  
Vol 13 (16) ◽  
pp. 3394-3400 ◽  
Author(s):  
Frederick S. Hagen ◽  
Elton T. Young
Keyword(s):  
Molecular Species ◽  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Bacteriophage T7 ◽  
Messenger Ribonucleic Acid

Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis

Biochemistry ◽  
1968 ◽  
Vol 7 (2) ◽  
pp. 659-668 ◽  
Author(s):  
C. Wesley. Dingman ◽  
Andrew C. Peacock
Keyword(s):  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Analytical Studies

scholarly journals Rapid, simple method of preparing rotaviral double-stranded ribonucleic acid for analysis by polyacrylamide gel electrophoresis.

1981 ◽  
Vol 14 (3) ◽  
pp. 273-280 ◽  
Author(s):  
K W Theil ◽  
C M McCloskey ◽  
L J Saif ◽  
D R Redman ◽  
E H Bohl ◽  
...  
Keyword(s):  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Simple Method

scholarly journals Ribonucleic acid synthesis by spermatozoa from the rat and hamster

1973 ◽  
Vol 133 (4) ◽  
pp. 635-639 ◽  
Author(s):  
Julia MacLaughlin ◽  
Charles Terner
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Acid Synthesis ◽  
Polyacrylamide Gel

Spermatozoa from the cauda of the epididymis of the hamster and rat were incubated with [5-3H]uridine and glucose. By using a procedure avoiding bacterial and other cellular contamination, sonic extracts were prepared and digested with deoxyribonuclease and Pronase. Radioactive RNA of high molecular weight was isolated by two methods: (a) gel filtration on Sephadex G-75 columns and (b) polyacrylamide-gel electrophoresis in which it migrated in the region of 28S and 23S RNA markers. The macromolecules were alkali-labile and hydrolysed by ribonuclease. From 3H radioactivity and E260 of the isolated RNA the rate of incorporation of uridine into RNA of spermatozoa was calculated to be 0.1–0.5nmol/h per mg of RNA.

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