Analysis of the rapidly synthesized ribonucleic acid of the normal human lymphocyte by agarose-polyacrylamide gel electrophoresis

Biochemistry ◽  
1971 ◽  
Vol 10 (9) ◽  
pp. 1733-1740 ◽  
Author(s):  
Paul E. Neiman ◽  
Patrick H. Henry
Keyword(s):  
Human Lymphocyte ◽  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Normal Human

Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis

Biochemistry ◽  
1968 ◽  
Vol 7 (2) ◽  
pp. 659-668 ◽  
Author(s):  
C. Wesley. Dingman ◽  
Andrew C. Peacock
Keyword(s):  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Analytical Studies

Re-evaluation of normal human urinary proteins fractionated by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis

1988 ◽  
Vol 172 (1) ◽  
pp. 77-84 ◽  
Author(s):  
Kiyoko Sano Shiba ◽  
Kiyoko Kanamori ◽  
Hiroko Cho ◽  
Noriko Furuhata ◽  
Tomiko Harada ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Urinary Proteins ◽  
One Dimensional ◽  
Normal Human

scholarly journals A mucus-secreting human colonic cancer cell line. Purification and partial characterization of the secreted mucins

1989 ◽  
Vol 258 (3) ◽  
pp. 793-799 ◽  
Author(s):  
J J Maoret ◽  
J Font ◽  
C Augeron ◽  
P Codogno ◽  
C Bauvy ◽  
...  
Keyword(s):  
Cell Line ◽  
Conditioned Medium ◽  
Gel Electrophoresis ◽  
Polyclonal Antibodies ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cancer Cell Line ◽  
Monosaccharide Composition ◽  
Polyacrylamide Gel ◽  
Antigenic Determinants ◽  
Normal Human

A stably differentiated clonal derivative (Cl.16E) of the human colonic adenocarcinoma cell line HT29 secretes in culture high-Mr glycoproteins that were purified from the serum-free conditioned medium by preparative SDS/polyacrylamide-gel electrophoresis. Analysis of the oligosaccharides released from the [3H]glucosamine-labelled high-Mr glycoproteins by alkaline-borohydride treatment showed that this material consisted of O-linked oligosaccharides (without any detectable N-linked oligosaccharides) that were eluted as three fractions from Bio-Gel P-6 columns. The main oligosaccharide fraction obtained after such treatment and desialylation was eluted together with a six-unit glucose polymer from a Bio-Gel P-4 column. Polyclonal antibodies were raised against the high-Mr glycoproteins, and in immunoblot analysis they reacted specifically with the high-Mr glycoproteins present in the conditioned medium. Furthermore, immunohistochemical staining of sections in paraffin wax revealed that these antibodies labelled normal human gastrointestinal mucins. We conclude that (1) the high-Mr glycoproteins prepared by SDS/polyacrylamide-gel electrophoresis are pure mucus glycoproteins on the basis of sensitivity to alkaline-borohydride treatment, monosaccharide composition and immunochemical and immunohistological findings, and (2) these mucins have antigenic determinants in common with the normal human gastrointestinal mucins.

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