Effect of glucocorticoids on hexose uptake by mouse fibroblasts in vitro

Biochemistry ◽  
1971 ◽  
Vol 10 (2) ◽  
pp. 277-284 ◽  
Author(s):  
William B. Pratt ◽  
Jean G. Gray ◽  
Lewis Aronow
Keyword(s):  
Mouse Fibroblasts ◽  
Hexose Uptake

Antioxidant potential of Arnica montana and Urtica dioica hydroalcoholic extracts on mouse fibroblasts in vitro

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
L Moldovan ◽  
O Craciunescu ◽  
L Toma ◽  
A Gaspar ◽  
D Constantin
Keyword(s):  
Urtica Dioica ◽  
Antioxidant Potential ◽  
Mouse Fibroblasts ◽  
Arnica Montana

scholarly journals The Effect of Glucocorticoids on Protein and Nucleic Acid Synthesis in Mouse Fibroblasts Growing in Vitro

1966 ◽  
Vol 241 (22) ◽  
pp. 5244-5250
Author(s):  
William B. Pratt ◽  
Lewis Aronow
Keyword(s):  
Nucleic Acid ◽  
Acid Synthesis ◽  
Nucleic Acid Synthesis ◽  
Mouse Fibroblasts

scholarly journals Phosphorylation by Cyclin C/Cyclin-Dependent Kinase 2 following Mitogenic Stimulation of Murine Fibroblasts Inhibits Transcriptional Activity of LSF during G1 Progression

2009 ◽  
Vol 29 (9) ◽  
pp. 2335-2345 ◽  
Author(s):  
Utsav H. Saxena ◽  
Christina M. H. Powell ◽  
Jill K. Fecko ◽  
Roxanne Cacioppo ◽  
Hubert S. Chou ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Target Genes ◽  
Cyclin Dependent Kinase ◽  
Mitogenic Stimulation ◽  
Mouse Fibroblasts ◽  
Cyclin C ◽  
Murine Fibroblasts ◽  
Interfering Rna ◽  
Stimulation Of

ABSTRACT Transcription factor LSF is required for progression from quiescence through the cell cycle, regulating thymidylate synthase (Tyms) expression at the G1/S boundary. Given the constant level of LSF protein from G0 through S, we investigated whether LSF is regulated by phosphorylation in G1. In vitro, LSF is phosphorylated by cyclin E/cyclin-dependent kinase 2 (CDK2), cyclin C/CDK2, and cyclin C/CDK3, predominantly on S309. Phosphorylation of LSF on S309 is maximal 1 to 2 h after mitogenic stimulation of quiescent mouse fibroblasts. This phosphorylation is mediated by cyclin C-dependent kinases, as shown by coimmunoprecipitation of LSF and cyclin C in early G1 and by abrogation of LSF S309 phosphorylation upon suppression of cyclin C with short interfering RNA. Although mouse fibroblasts lack functional CDK3 (the partner of cyclin C in early G1 in human cells), CDK2 compensates for this absence. By transient transfection assays, phosphorylation at S309, mediated by cyclin C overexpression, inhibits LSF transactivation. Moreover, overexpression of cyclin C and CDK3 inhibits induction of endogenous Tyms expression at the G1/S transition. These results identify LSF as only the second known target (in addition to pRb) of cyclin C/CDK activity during progression from quiescence to early G1. Unexpectedly, this phosphorylation prevents induction of LSF target genes until late G1.

scholarly journals Experimental in vitro Comparative Study of Chromovitectomy Agents Cyto- and Phototoxicity

2020 ◽  
Vol 17 (3) ◽  
pp. 473-480
Author(s):  
N. M. Kislitsyna ◽  
S. V. Novikov ◽  
N. V. Perova ◽  
S. V. Kolesnik ◽  
A. I. Kolesnik ◽  
...  
Keyword(s):  
Safety Profile ◽  
Cytotoxic Effect ◽  
Vitreous Body ◽  
Fibroblast Cell ◽  
Negative Control ◽  
Mouse Fibroblast ◽  
Test Plate ◽  
Mouse Fibroblasts ◽  
Nih 3T3

Intravitreal use of vital dyes in combination with the action of endoillumination can induce a cyto- and phototoxic effect on posterior eye segment structures. The search for a staining agent with a maximum safety profile to retinal structures, intensively and selectively coloring vitreous body and vitreoretinal interface structure, remains relevant.Objective: to determine comparative viability of NIH / 3T3 mouse fibroblast cell culture with traditional agents for chromovitrectomy and “Vitreocontrast” suspension with and without endovitreal illumination.Materials and methods. NIH / 3T3 mouse fibroblast cultures contacted with agents for chromovitrectomy (MembraneBlue® Dual, Triamcinolone acetonide, “Vitreocontrast” suspension) and the corresponding controls in a volume of 50 μl / well. The test plate was irradiated with a Photon II illuminator (Synergetics, USA), working distance of 5 mm. The control tablet with the introduced preparations was not exposed to light. Next, the cells were washed and incubated, after which the morphology and lysis of the cells, as well as the number of proliferating relatively negative control of fibroblasts, were evaluated using the vital dye PrestoBlue Cell Viability Reagent. Negative control was the complete growth medium for the cultivation of mouse fibroblasts of the NIH / 3T3 line. The results of the cytotoxic reaction of a culture of mouse fibroblasts of the NIH / 3T3 line were interpreted using the table “The degree of cell response”.Results. Studies have shown that exposure to a source of endovitual illumination does not affect the cytotoxic effect of TA suspension and MembraneBlue® Dual dye. The TA suspension, both after light source and without it, has a moderate cytotoxic effect, and MembraneBlue® Dual has no cytotoxic effect on the culture of mouse fibroblasts of the NIH / 3T3 strain. Without light, “Vitreocontrast” suspension does not have cytotoxic effect on mouse fibroblasts culture NIH / 3T3 line. Light irradiation for 1 h increases the cytotoxicity of “Vitreocontrast” suspension to the level of unsharp cytotoxicity allowed by ISO Standard 10993-5-2011.Conclusion. The safety profile of MembraneBlue® Dual and “Vitreocontrast” suspension allows them to be recommended for use in endovitreal surgery. The cyto- and phototoxicity demonstrated in the experiment with TA suspension can reduce the functional outcomes of retinal surgery. 

The influence of MuLV and SV40 viruses on senescence in mouse fibroblasts in vitro

1976 ◽  
Vol 32 (1) ◽  
pp. 98-99 ◽  
Author(s):  
R. S. U. Baker
Keyword(s):  
Mouse Fibroblasts

Specific binding of glucocorticoids in vitro in the soluble fraction of mouse fibroblasts

Biochemistry ◽  
1972 ◽  
Vol 11 (8) ◽  
pp. 1401-1410 ◽  
Author(s):  
William B. Pratt ◽  
Douglas N. Ishii
Keyword(s):  
Soluble Fraction ◽  
Specific Binding ◽  
Mouse Fibroblasts
Sign in / Sign up

Export Citation Format

Share Document