Micelle-Induced Folding of Spinach Thylakoid Soluble Phosphoprotein of 9 kDa and Its Functional Implications†,‡

Information regarding the normal morphology of uterine blood vessels at ultrastructural level in mammals is scarce Electron microscopy studies dealing with endometrial vasculature despite the functional implications due to hormone priming are not available. Light microscopy observations with combined injection of dyes and microradiography along with histochemical studies does not enable us to know the detailed fine structure of the possible various types of blood vessels in this tissue. The present work has been designed to characterize the blood vessels of endometrium of mice as well as the behavior of the endothelium to injection of low molecular weight proteins during the normal estrous cycle in this animal. One hundred and forty female albino mice were sacrificed after intravascular injection of horse radish peroxidase (HRP) at 30 seconds, 5, 15, 30 and 60 minutes.

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Intraindividual Variability and Stability of Affect and Well-Being

GeroPsych ◽

10.1024/1662-9647/a000094 ◽

2013 ◽

Vol 26 (3) ◽

pp. 185-199 ◽

Cited By ~ 40

Author(s):

Christina Röcke ◽

Annette Brose

Keyword(s):

Age Differences ◽

Well Being ◽

Subjective Well Being ◽

Emotional Experiences ◽

Short Term ◽

Regulatory Processes ◽

Functional Implications ◽

Methodological Approaches ◽

Underlying Processes

Whereas subjective well-being remains relatively stable across adulthood, emotional experiences show remarkable short-term variability, with younger and older adults differing in both amount and correlates. Repeatedly assessed affect data captures both the dynamics and stability as well as stabilization that may indicate emotion-regulatory processes. The article reviews (1) research approaches to intraindividual affect variability, (2) functional implications of affect variability, and (3) age differences in affect variability. Based on this review, we discuss how the broader literature on emotional aging can be better integrated with theories and concepts of intraindividual affect variability by using appropriate methodological approaches. Finally, we show how a better understanding of affect variability and its underlying processes could contribute to the long-term stabilization of well-being in old age.

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Glucokinase gene structure. Functional implications of molecular genetic studies

Diabetes ◽

10.2337/diabetes.39.5.523 ◽

1990 ◽

Vol 39 (5) ◽

pp. 523-527 ◽

Cited By ~ 21

Author(s):

M. A. Magnuson

Keyword(s):

Gene Structure ◽

Molecular Genetic ◽

Glucokinase Gene ◽

Genetic Studies ◽

Functional Implications

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Hierarchy of π-Stacking Determines the Conformational Preference of Bis-Squaraines

10.26434/chemrxiv.13041830.v1 ◽

2020 ◽

Author(s):

Abhishek Singh ◽

Reman K. Singh ◽

G Naresh Patwari

Keyword(s):

Hydrogen Bonding ◽

Rational Design ◽

Biological Molecules ◽

Conformational Space ◽

X Ray Crystallography ◽

Simple Strategy ◽

Induced Folding ◽

Π Stacking ◽

Varying Length ◽

Dynamics Simulations

The rational design of conformationally controlled foldable modules can lead to a deeper insight into the conformational space of complex biological molecules where non-covalent interactions such as hydrogen bonding and π-stacking are known to play a pivotal role. Squaramides are known to have excellent hydrogen bonding capabilities and hence, are ideal molecules for designing foldable modules that can mimic the secondary structures of bio-molecules. The π-stacking induced folding of bis-squaraines tethered using aliphatic primary and secondary-diamine linkers of varying length is explored with a simple strategy of invoking small perturbations involving the length linkers and degree of substitution. Solution phase NMR investigations in combination with molecular dynamics simulations suggest that bis-squaraines predominantly exist as extended conformations. Structures elucidated by X-ray crystallography confirmed a variety of folded and extended secondary conformations including hairpin turns and 𝛽-sheets which are determined by the hierarchy of π-stacking relative to N–H···O hydrogen bonds.

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Hierarchy of π-Stacking Determines the Conformational Preference of Bis-Squaraines

10.26434/chemrxiv.13041830 ◽

2020 ◽

Author(s):

Abhishek Singh ◽

Reman K. Singh ◽

G Naresh Patwari

Keyword(s):

Hydrogen Bonding ◽

Rational Design ◽

Biological Molecules ◽

Conformational Space ◽

X Ray Crystallography ◽

Simple Strategy ◽

Induced Folding ◽

Π Stacking ◽

Varying Length ◽

Dynamics Simulations

The rational design of conformationally controlled foldable modules can lead to a deeper insight into the conformational space of complex biological molecules where non-covalent interactions such as hydrogen bonding and π-stacking are known to play a pivotal role. Squaramides are known to have excellent hydrogen bonding capabilities and hence, are ideal molecules for designing foldable modules that can mimic the secondary structures of bio-molecules. The π-stacking induced folding of bis-squaraines tethered using aliphatic primary and secondary-diamine linkers of varying length is explored with a simple strategy of invoking small perturbations involving the length linkers and degree of substitution. Solution phase NMR investigations in combination with molecular dynamics simulations suggest that bis-squaraines predominantly exist as extended conformations. Structures elucidated by X-ray crystallography confirmed a variety of folded and extended secondary conformations including hairpin turns and 𝛽-sheets which are determined by the hierarchy of π-stacking relative to N–H···O hydrogen bonds.

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Faculty Opinions recommendation of Osmolyte-induced folding of an intrinsically disordered protein: folding mechanism in the absence of ligand.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.4040956.3812054 ◽

2010 ◽

Author(s):

Kunihiro Kuwajima

Keyword(s):

Protein Folding ◽

Intrinsically Disordered Protein ◽

Intrinsically Disordered ◽

Disordered Protein ◽

Folding Mechanism ◽

Induced Folding

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Gravity-induced folding off a gneiss dome complex, Rincon Mountains, Arizona: Discussion and reply

Geological Society of America Bulletin ◽

10.1130/0016-7606(1977)88<1211:gfoagd>2.0.co;2 ◽

1977 ◽

Vol 88 (8) ◽

pp. 1211 ◽

Cited By ~ 5

Author(s):

C. H. THORMAN

Keyword(s):

Gneiss Dome ◽

Induced Folding ◽

Rincon Mountains

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A comprehensive annotation dataset of intact LTR retrotransposons of 300 plant genomes

Scientific Data ◽

10.1038/s41597-021-00968-x ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Shan-Shan Zhou ◽

Xue-Mei Yan ◽

Kai-Fu Zhang ◽

Hui Liu ◽

Jie Xu ◽

...

Keyword(s):

Evolutionary Dynamics ◽

Age Determination ◽

Genome Plasticity ◽

Ltr Retrotransposons ◽

Functional Variation ◽

Plant Genomes ◽

Sequencing Technologies ◽

Plant Groups ◽

Functional Implications ◽

Classification Information

AbstractLTR retrotransposons (LTR-RTs) are ubiquitous and represent the dominant repeat element in plant genomes, playing important roles in functional variation, genome plasticity and evolution. With the advent of new sequencing technologies, a growing number of whole-genome sequences have been made publicly available, making it possible to carry out systematic analyses of LTR-RTs. However, a comprehensive and unified annotation of LTR-RTs in plant groups is still lacking. Here, we constructed a plant intact LTR-RTs dataset, which is designed to classify and annotate intact LTR-RTs with a standardized procedure. The dataset currently comprises a total of 2,593,685 intact LTR-RTs from genomes of 300 plant species representing 93 families of 46 orders. The dataset is accompanied by sequence, diverse structural and functional annotation, age determination and classification information associated with the LTR-RTs. This dataset will contribute valuable resources for investigating the evolutionary dynamics and functional implications of LTR-RTs in plant genomes.

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