Antioxidant Defense during Recovery of Resurrection Plant Haberlea rhodopensis from Drought- and Freezing-Induced Desiccation

In this study, the contribution of nonenzymatic (ascorbate, glutathione) and enzymatic antioxidants (superoxide dismutase, catalase, glutathione reductase, glutathione S-transferase) in the first hours of recovery of the resurrection plant Haberlea rhodopensis from drought- and freezing-induced desiccation was assessed. The initial stage of recovery after desiccation is critical for plants, but less investigated. To better understand the alterations in the activity of antioxidant enzymes, their isoenzyme patterns were determined. Our results showed that ascorbate content remained high during the first 9 h of rehydration of desiccated plants and declined when the leaves′ water content significantly increased. The glutathione content remained high at the first hour of rehydration and then strongly decreased. The changes in ascorbate and glutathione content during recovery from drought- and freezing-induced desiccation showed great similarity. At the beginning of rehydration (1–5 h), the activities of antioxidant enzymes were significantly increased or remained as in dry plants. During 7–24 h of rehydration, certain differences in the enzymatic responses between the two plant groups were registered. The maintenance of a high antioxidant activity and upregulation of individual enzyme isoforms indicated their essential role in protecting plants from oxidative damage during the onset of recovery.

Synergistic Association With Root Endophytic Fungi Improves Morpho-Physiological and Biochemical Responses of Chenopodium quinoa to Salt Stress

Symbiotic associations with microbes can contribute to mitigating abiotic environmental stress in plants. In this study, we investigated individual and interactive effects of two root endophytic fungal species on physiological and biochemical mechanisms of the crop Chenopodium quinoa in response to salinity. Fungal endophytes (FE) Talaromyces minioluteus and Penicillium murcianum, isolated from quinoa plants that occur naturally in the Atacama Desert, were used for endophyte inoculation. A greenhouse experiment was developed using four plant groups: (1) plants inoculated with T. minioluteus (E1+), (2) plants inoculated with P. murcianum (E2+), (3) plants inoculated with both fungal species (E1E2+), and (4) non-inoculated plants (E-). Plants from each group were then assigned to either salt (300 mM) or control (no salt) treatments. Differences in morphological traits, photosynthesis, stomatal conductance, transpiration, superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase, (POD), phenylalanine ammonia-lyase (PAL), phenolic content, and lipid peroxidation between plant groups under each treatment were examined. We found that both endophyte species significantly improved morphological and physiological traits, including plant height, number of shoots, photosynthesis, stomatal conductance, and transpiration, in C. quinoa in response to salt, but optimal physiological responses were observed in E1E2+ plants. Under saline conditions, endophyte inoculation improved SOD, APX, and POD activity by over 50%, and phenolic content by approximately 30%, with optimal enzymatic responses again observed in E1E2+ plants. Lipid peroxidation was significantly lower in inoculated plants than in non-inoculated plants. Results demonstrate that both endophyte species enhanced the ability of C. quinoa to cope with salt stress by improving antioxidative enzyme and non-enzyme systems. In general, both FE species interacting in tandem yielded better morphological, physiological, and biochemical responses to salinity in quinoa than inoculation by a single species in isolation. Our study highlights the importance of stress-adapted FE as a biological agent for mitigating abiotic stress in crop plants.

Ethno-botanical uses and taxonomic composition of some Aju Mbaise (Mbaise wraps)

This document focuses on the ethnobotanical uses and taxonomic composition of some Aju Mbaise (Mbaise wraps). A total of 31 plant species belonging to 24 families were identified in the wraps collected from the study area. These plants are used in the treatment of ailments such as hypertension, typhoid, malaria, boost fertility in females, post-partum (immediately after birth), treatment of diabetes, treatment of arthritis, and induce weight loss. Annonaceae family constituted 9.68% of the total plant family, followed by Acanthaceae, Combretaceae, Fabaceae, Malvaceae, and Rubiaceae with 6.45% each. Among the plant species, Cnestis ferruginea (Connaraceae) occurred in five wraps. This is followed by Alternanthera bettzickiana (Amaranthaceae), Craterispermum cerinanthum (Rubiaceae), and Napoleona imparalis (Lecythidaceae) found in four wraps while Acanthus montanus (Acanthaceae), Barteria nigritana (Passifloraceae), Nephrolepis exaltata (Nephrolepidaceae), Oxytenanthera abyssinica (Poaceae), Palisota hirsuta (Commelinaceae), Scleria naumanniana (Cyperaceae), and Sida rhomboidea (Malvaceae) are in three ailments each. These plant species and families with high occurrence are the most effectively used plant groups in the study for the treatment of these diseases.

CLASSIFICATION OF LANDS OF REMOTE SENSITIVE DATA BY NDVI METHOD IN SMART AGRICULTURE

A large share of the earth's surface is observed with remote sensing technology. Thanks to the data obtained from this process, information about the observed lands is obtained. In this study, NDVI (normalized difference), which is developed by applying mathematical operations on the reflection values of plants at different wavelengths from remote sensing technology and different application areas of this technology, electromagnetic rays, and spectral reflection values, and which is used as a method that provides a value expressing vegetation density. Vegetation index) method, NDVI value, and plant groups analyzed according to this value, sample MATLAB applications related to the NDVI method are mentioned. -Green-Blue) image of visible red and infrared regions, histogram graph showing the relationships between the intensities of values in NIR (near-infrared) and Red (visible Red) bands, NDVI image, and threshold function at the end. The NDVI image was obtained by using the direction (to detect areas that may have vegetation) is shown.

Ecosystem dynamics after forest fire. Blideen Atlas case

Human disturbances affect the majority of terrestrial ecosystems. The radical changes in the behaviour of ecological systems, partial or total destruction of plant biomass, often with the death of fundamental entities. Of these disturbances, fires affect many terrestrial ecosystems, particularly forests, by changing their floristic composition, their structure and their functioning; the global average annual forest area burned is estimated at 65 million ha. The Mediterranean basin, a hotspot, annually loses between 0.5 and 1 million hectares of forest. In Algeria, the annual average of areas destroyed by fire is between 45,000 and 50,000 ha. Our work set out to study the changes in the plant coverage of the land one year after the passage of fire. We studied the effects at the floristic, ecological and dynamic level in a forest ecosystem located in the north of Algeria (Atlas Blideen). The phytoecological inventory of vegetation (74 surveys, 162 species) was carried out from subjective sampling. To highlight the different groupings in the study area, classical statistical treatments (factorial analysis of correspondences) were applied to the floristic and ecological data. The results of computer processing made it possible to individualize and classify four plant groups according to the degree of the fire. The qualitative and quantitative analysis of these groups shows a therophytization of the flora, due to the regression of the forest cover (disappearance of the phanerophytes) caused by the passage of fire, with a floral procession represented mainly by Asteraceae, Poaceae and Fabaceae and an index disturbance which greatly exceeds 50%. However, some tree and shrub taxa such as cork oak, holm oak and Pistacia lentiscus tree have the capacity to reappear by rejecting the calcined stumps.

Ecological aspect of the development of soil-forming processes at the post-technogenic stage

The relevance of the study is caused by the problem of the global anthropogenic transformation of natural landscapes in Kuzbass as a result of the development of mining. The rate of reclamation of disturbed lands lags behind the rate and scale of their formation, and most of the territory is self-recovering. This paper is aimed at studying the ecological conditions of the natural environment and technogenic factors affecting the dynamics of soil-forming processes and the formation of phytocenoses in disturbed territories, which differ in the way of formation at the technogenic stage. For the first time, some physicochemical parameters of technozems were obtained. Like ebriozems, they are formed in the post-technogenic phase of the development of a technogenic landscape under the conditions of its self-growth. The similarities and differences in the properties of technozems and ebriozems formed in the same natural and climatic conditions of the southern forest-steppe zone of Kuzbass have been revealed. It was found that the rate of their transformation, expressed in the formation of the soil profile, depends on the conditions of the relief and underlying rocks created at the technogenic stage. The presence of a preserved layer of potentially fertile rocks and the absence of slope surfaces led to the formation of the frontal soil and vegetation cover of the technozem; under other conditions, the formation of soils and plant groups occurs fragmentarily, which leads to the asynchronous development of the functions and modes of the technogenic landscape.

Ostracods had colonized estuaries by the late Silurian

The fossil record of terrestrialization documents notable shifts in the environmental and physiological tolerances of many animal and plant groups. However, for certain significant components of modern freshwater and terrestrial environments, the transition out of marine settings remains largely unconstrained. Ostracod crustaceans occupy an exceptional range of modern aquatic environments and are invaluable palaeoenvironmental indicators in the fossil record. However, pre-Carboniferous records of supposed non-marine and marginal marine ostracods are sparse, and the timing of their marine to non-marine transition has proven elusive. Here, we reassess the early environmental history of ostracods in light of new assemblages from the late Silurian of Vietnam. Two, low diversity but distinct ostracod assemblages are associated with estuarine deposits. This occurrence is consistent with previous incidental reports of ostracods occupying marginal and brackish settings through the late Silurian and Devonian. Therefore, ostracods were pioneering the occupation of marginal marine and estuarine settings 60 Myr before the Carboniferous and they were a component of the early phase of transition from marine to non-marine environments.

Amarakaeri: Connecting Biodiversity | Conectando la Biodiversidad

<p><b><i>This digital monograph is made available by the publisher. Print copies are available for purchase through our distributor, Penguin Random House and on Amazon.com</i></b></p> <p><em><b> </b></em></p><p>Highlighting the enormous biodiversity of the Amarakaeri Communal Reserve (ACR) and the critical role this protected area plays in the conservation of Madre de Dios, in southeastern Peru, with more than 1,700 vivid photographs, <i>Amarakaeri: Connecting Biodiversity</i> offers readers a glimpse into the extensive research conducted by scholars from the Smithsonian National Zoo and Conservation Biology Institute and their Peruvian counterparts. For three years, scientists and local experts had the extraordinary opportunity to collect data at the premontane forests of the ACR to determine potential impacts of an exploratory gas platform on selected animal and plant groups. <i>Amarakaeri</i> also portrays the main threats to the ACR and presents a vision for the region’s future.</p><p>Destacando la enorme biodiversidad de la Reserva Comunal de Amarakaeri (RCA) y el papel crítico que esta área protegida juega en la conservación de Madre de Dios, en el sureste de Perú, <i>Amarakaeri: Conectando la Biodiversidad</i> ofrece a los lectores una mirada rápida a la extensa investigación realizada por académicos del Smithsonian National Zoo y del Conservation Biology Institute junto con sus homólogos peruanos. Durante tres años, los científicos y expertos locales tuvieron la extraordinaria oportunidad de compilar datos en los bosques premontanos de la RCA para determinar los posibles impactos de una plataforma exploratoria de gas en grupos seleccionados de animales y plantas. <i>Amarakaeri</i> además retrata las principales amenazas a la reserva y presenta una visión para el futuro de la región.<br></p><div></div>

The Evolution of Wahlenbergia (Campanulaceae) in Australasia

<p>Wahlenbergia is a large genus of flowering plants within the family Campanulaceae. In this thesis the first molecular phylogeny of Wahlenbergia was reconstructed from approximately 20% of the genus, based on the nuclear ribosomal ITS (nrITS) DNA marker and the chloroplast trnL-F DNA marker, with samples from South Africa, Europe, Australia and New Zealand. Additionally a large phylogeny with increased within-species sampling focusing on addressing taxonomic questions among the 45 Australasian species of Wahlenbergia was also reconstructed based on nrITS and trnL-F, plus an additional chloroplast DNA marker, trnK. Relationships and species limits of the New Zealand species of Wahlenbergia were further analysed using amplified fragment length polymorphisms (AFLPs). Wahlenbergia was found to be polyphyletic, though most of the species form a clade. Tree topologies and molecular dating analysis showed that the genus originated in South Africa about 16.2 million years ago (mya), then dispersed to Australasia before radiating there about 3.7 mya, thus refuting the hypothesis of Gondwanan vicariance for the Australasian species. Two dispersals from Australia to New Zealand are hypothesised, one leading to a radiation of species with the rhizomatous growth from about 1.0 mya and the other leading to a radiation of species with the radicate growth form 0.49 mya, although the radicate species might not form a clade. Low levels of genetic variation among individuals from Australia and New Zealand was revealed with all markers, and the phylogenies were poorly resolved as a result. The low genetic diversity is probably due to rapid and recent evolution during a period of geological and climatic change, coupled with incomplete lineage sorting and hybridisation. Phylogenies reconstructed using AFLPs were also poorly resolved, although AFLPs were found to be useful for species delimitation, as has been shown in studies of other plant groups. Despite the poor resolution, several morphological species and subspecies were recovered as monophyletic with DNA sequence data, notably the morphologically distinctive New Zealand W. cartilaginea, W. matthewsii and W. congesta subsp. congesta. Further research into species boundaries within the W. albomarginata/W. pygmaea complex is needed. Members of the New Zealand lowland radicate W. gracilis complex may all belong to the same morphologically variable species, although further research is needed to justify such a taxonomic change. The other New Zealand radicate species, W. vernicosa, is probably a separately evolving lineage, and is not conspecific with the Australian W. littoricola.</p>

The Evolution of Wahlenbergia (Campanulaceae) in Australasia

<p>Wahlenbergia is a large genus of flowering plants within the family Campanulaceae. In this thesis the first molecular phylogeny of Wahlenbergia was reconstructed from approximately 20% of the genus, based on the nuclear ribosomal ITS (nrITS) DNA marker and the chloroplast trnL-F DNA marker, with samples from South Africa, Europe, Australia and New Zealand. Additionally a large phylogeny with increased within-species sampling focusing on addressing taxonomic questions among the 45 Australasian species of Wahlenbergia was also reconstructed based on nrITS and trnL-F, plus an additional chloroplast DNA marker, trnK. Relationships and species limits of the New Zealand species of Wahlenbergia were further analysed using amplified fragment length polymorphisms (AFLPs). Wahlenbergia was found to be polyphyletic, though most of the species form a clade. Tree topologies and molecular dating analysis showed that the genus originated in South Africa about 16.2 million years ago (mya), then dispersed to Australasia before radiating there about 3.7 mya, thus refuting the hypothesis of Gondwanan vicariance for the Australasian species. Two dispersals from Australia to New Zealand are hypothesised, one leading to a radiation of species with the rhizomatous growth from about 1.0 mya and the other leading to a radiation of species with the radicate growth form 0.49 mya, although the radicate species might not form a clade. Low levels of genetic variation among individuals from Australia and New Zealand was revealed with all markers, and the phylogenies were poorly resolved as a result. The low genetic diversity is probably due to rapid and recent evolution during a period of geological and climatic change, coupled with incomplete lineage sorting and hybridisation. Phylogenies reconstructed using AFLPs were also poorly resolved, although AFLPs were found to be useful for species delimitation, as has been shown in studies of other plant groups. Despite the poor resolution, several morphological species and subspecies were recovered as monophyletic with DNA sequence data, notably the morphologically distinctive New Zealand W. cartilaginea, W. matthewsii and W. congesta subsp. congesta. Further research into species boundaries within the W. albomarginata/W. pygmaea complex is needed. Members of the New Zealand lowland radicate W. gracilis complex may all belong to the same morphologically variable species, although further research is needed to justify such a taxonomic change. The other New Zealand radicate species, W. vernicosa, is probably a separately evolving lineage, and is not conspecific with the Australian W. littoricola.</p>