scholarly journals The Membrane Interface Dictates Different Anchor Roles for “Inner Pair” and “Outer Pair” Tryptophan Indole Rings in Gramicidin A Channels

Biochemistry ◽  
2011 ◽  
Vol 50 (22) ◽  
pp. 4855-4866 ◽  
Author(s):  
Hong Gu ◽  
Kevin Lum ◽  
Jung H. Kim ◽  
Denise V. Greathouse ◽  
Olaf S. Andersen ◽  
...  
Keyword(s):  
Gramicidin A ◽  
Membrane Interface ◽  
Outer Pair

Two-Dimensional Crystallization of Tetanus Toxin

1985 ◽  
Vol 43 ◽  
pp. 528-529
Author(s):  
Jeffry A. Reidler ◽  
John P. Robinson
Keyword(s):  
Electron Microscopy ◽  
Tetanus Toxin ◽  
Structural Information ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Membrane Interface ◽  
3D Reconstructions ◽  
Cellular Receptors ◽  
Computer Reconstruction ◽  
2D Crystals

We have prepared two-dimensional (2D) crystals of tetanus toxin using procedures developed by Uzgiris and Kornberg for the directed production of 2D crystals of monoclonal antibodies at an antigen-phospholipid monolayer interface. The tetanus toxin crystals were formed using a small mole fraction of the natural receptor, GT1, incorporated into phosphatidyl choline monolayers. The crystals formed at low concentration overnight. Two dimensional crystals of this type are particularly useful for structure determination using electron microscopy and computer image refinement. Three dimensional (3D) structural information can be derived from these crystals by computer reconstruction of photographs of toxin crystals taken at different tilt angles. Such 3D reconstructions may help elucidate the mechanism of entry of the enzymatic subunit of toxins into cells, particularly since these crystals form directly on a membrane interface at similar concentrations of ganglioside GT1 to the natural cellular receptors.

Phase Transitions in Phospholipid Monolayers Studied by Atomic Force Microscopy and Langmuir-Blodgett Technique

2008 ◽  
Vol 59 (11) ◽  
Author(s):  
Maria Tomoaia-Cotisel ◽  
Aurora Mocanu
Keyword(s):  
Atomic Force Microscopy ◽  
Lipid Membrane ◽  
Phase Behaviour ◽  
Membrane Interface ◽  
Force Microscopy ◽  
Langmuir Blodgett ◽  
Atomic Force ◽  
Phospholipid Monolayers ◽  
Air Water Interface ◽  
Condensed Liquid

The phase behaviour and surface structure of dipalmitoyl phosphatidyl choline (DPPC) monolayers at the air/water interface, in the absence and the presence of procaine, have been investigated by Langmuir-Blodgett (LB) technique and atomic force microscopy. The LB films were transferred on mica, at a controlled surface pressure, characteristic for the expanded liquid to condensed liquid phase transition of pure DPPC monolayers. The results indicate that procaine penetrates into and specifically interacts with phospholipid monolayers stabilizing the lipid membrane interface.

Influence of acylation on the channel characteristics of gramicidin A

Biochemistry ◽  
1992 ◽  
Vol 31 (32) ◽  
pp. 7320-7324 ◽  
Author(s):  
T. C. B. Vogt ◽  
J. A. Killian ◽  
B. De Kruijff ◽  
O. S. Andersen
Keyword(s):  
Gramicidin A ◽  
Channel Characteristics

Mixed Langmuir monolayers of gramicidin A and fluorinated alcohols

2007 ◽  
Vol 313 (2) ◽  
pp. 600-607 ◽  
Author(s):  
Marcin Broniatowski ◽  
Katarzyna Obidowicz ◽  
Nuria Vila Romeu ◽  
Elżbieta Broniatowska ◽  
Patrycja Dynarowicz-Łątka
Keyword(s):  
Langmuir Monolayers ◽  
Gramicidin A ◽  
Fluorinated Alcohols
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