On the Optimal Ratio of Heavy to Light Chain Genes for Efficient Recombinant Antibody Production by CHO Cells

2008 ◽  
Vol 21 (1) ◽  
pp. 122-133 ◽  
Author(s):  
Stefan Schlatter ◽  
Scott H. Stansfield ◽  
Diane M. Dinnis ◽  
Andrew J. Racher ◽  
John R. Birch ◽  
...  
Keyword(s):  
Light Chain ◽  
Antibody Production ◽  
Cho Cells ◽  
Recombinant Antibody ◽  
Optimal Ratio ◽  
Recombinant Antibody Production

scholarly journals Augmenting recombinant antibody production in HEK293E cells: Optimising transfection and culture parameters

2022 ◽  
Author(s):  
Zealyn Shi-Lin Heng ◽  
Joshua Yi Yeo ◽  
Darius Wen-Shuo Koh ◽  
Samuel Ken-En Gan ◽  
Wei-Li Ling
Keyword(s):  
Light Chain ◽  
Antibody Production ◽  
Recombinant Antibody ◽  
Cost Effective ◽  
Efficient Production ◽  
Effective Manner ◽  
Recombinant Antibody Production ◽  
Production Increase ◽  
Culture Parameters ◽  
Complexation Time

Abstract Background Optimising recombinant antibody production is important for cost-effective therapeutics and diagnostics. With impact on commercialisation, higher productivity beyond laboratory scales is highly sought, where efficient production can also accelerate antibody characterisations and investigations. Methods Investigating HEK293E cells for mammalian antibody production, various transfection and culture parameters were systematically analysed for antibody light chain production before evaluating them for whole antibody production. Transfection parameters investigated include seeding cell density, the concentration of the transfection reagent and DNA, complexation time, temperature, and volume, as well as culture parameters such as medium replacement, serum deprivation, use of cell maintenance antibiotic, incubation temperature, medium volume, post-transfection harvest day and common nutrient supplements. Results Using 2 mL adherent HEK293E cell culture transfections with 25 kDa linear Polyethylenimine in the most optimised parameters, we demonstrated a ~ 2-fold production increase for light chain alone and for whole antibody production reaching 536 and 49 μg respectively in a cost-effective manner. With the addition of peptone, κ light chain increased by ~ 4-fold to 1032 μg while whole antibody increased to a lesser extent by ~ 2.5-fold to 51 μg, with benefits potentially for antibodies limited by their light chains in production. Conclusions Our optimised findings show promise for a more efficient and convenient antibody production method through transfection and culture optimisations that can be incorporated to scale up processes and with potential transferability to other mammalian-based recombinant protein production using HEK293E cells. Statement of Significance Recombinant antibody production is crucial for antibody research and development. Systematically investigating transfection and culture parameters such as PEI/DNA concentrations, complexation time, volume, and temperature, supplements, etc., we demonstrated a ~ 4-fold light chain alone production increase to 1032 μg and a 2.5-fold whole antibody production increase to 51 μg from 2 mL transfections.

Metabolite profiling of recombinant CHO cells: Designing tailored feeding regimes that enhance recombinant antibody production

2011 ◽  
Vol 108 (12) ◽  
pp. 3025-3031 ◽  
Author(s):  
Christopher A. Sellick ◽  
Alexandra S. Croxford ◽  
Arfa R. Maqsood ◽  
Gill Stephens ◽  
Hans V. Westerhoff ◽  
...  
Keyword(s):  
Antibody Production ◽  
Cho Cells ◽  
Metabolite Profiling ◽  
Recombinant Antibody ◽  
Recombinant Cho Cells ◽  
Feeding Regimes ◽  
Recombinant Antibody Production

Effects of glutamine and asparagine on recombinant antibody production using CHO-GS cell lines

2014 ◽  
Vol 30 (6) ◽  
pp. 1457-1468 ◽  
Author(s):  
Ping Xu ◽  
Xiao-Ping Dai ◽  
Erica Graf ◽  
Richard Martel ◽  
Reb Russell
Keyword(s):  
Cell Lines ◽  
Antibody Production ◽  
Recombinant Antibody ◽  
Recombinant Antibody Production

scholarly journals Screening for novel genes ofSaccharomyces cerevisiaeinvolved in recombinant antibody production

2016 ◽  
Vol 17 (1) ◽  
pp. fow104 ◽  
Author(s):  
Jorg C. de Ruijter ◽  
German Jurgens ◽  
Alexander D. Frey
Keyword(s):  
Antibody Production ◽  
Recombinant Antibody ◽  
Novel Genes ◽  
Recombinant Antibody Production

Economics and ecology: Modelling of continuous primary recovery and capture scenarios for recombinant antibody production

2020 ◽  
Vol 308 ◽  
pp. 87-95 ◽  
Author(s):  
A.L. Cataldo ◽  
D. Burgstaller ◽  
G. Hribar ◽  
A. Jungbauer ◽  
P. Satzer
Keyword(s):  
Antibody Production ◽  
Recombinant Antibody ◽  
Recombinant Antibody Production
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