Effect of Glucose or Glycerol as the Sole Carbon Source on Gene Expression from the Salmonella prpBCDE Promoter in Escherichia coli

2006 ◽  
Vol 22 (6) ◽  
pp. 1547-1551 ◽  
Author(s):  
S.K. Lee ◽  
J.D. Keasling
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Effect Of Glucose

Mannose utilization in Escherichia coli requires cyclic AMP but not an exogenous inducer

1980 ◽  
Vol 26 (12) ◽  
pp. 1508-1511 ◽  
Author(s):  
Ann D. E. Fraser ◽  
Hiroshi Yamazaki
Keyword(s):  
Escherichia Coli ◽  
Carbon Source ◽  
Cyclic Amp ◽  
Adenylyl Cyclase ◽  
Sole Carbon Source ◽  
Receptor Protein ◽  
Deficient Mutant ◽  
Phosphotransferase System ◽  
Cyclic Amp Receptor Protein ◽  
Cyclic Monophosphate

It has not been clarified whether the utilization of mannose by Escherichia coli requires adenosine 3′,5′-cyclic monophosphate (cyclic AMP). Using an adenylyl cyclase deficient mutant (CA8306B) and a cyclic AMP receptor protein (CRP) deficient mutant (5333B) we have shown that the utilization of mannose is dependent on the cyclic AMP–CRP complex. 2-Deoxyglucose (DG) is a nonmetabolizable glucose analog specific for the phosphotransferase system (PTS) which transports mannose (termed here PTSM). Growth of CA8306B on glycerol is unaffected by addition of the analog, whereas growth of the strain on glycerol plus cyclic AMP ceases im mediately upon addition of DG. These results suggest that the formation of PTSM is dependent on cyclic AMP. In addition, CA8306B grown on glycerol plus cyclic AMP can immediately utilize mannose when transferred to a medium containing mannose as a sole carbon source, whereas the same strain grown on glycerol without cyclic AMP cannot utilize mannose when so transferred. These results suggest that the formation of PTSM does not require an exogenous inducer.

scholarly journals Heterologous Production of 6-Deoxyerythronolide B in Escherichia coli through the Wood Werkman Cycle

Metabolites ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 228
Author(s):  
R. Axayacatl Gonzalez-Garcia ◽  
Lars K. Nielsen ◽  
Esteban Marcellin
Keyword(s):  
Escherichia Coli ◽  
Natural Products ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Structural Diversity ◽  
Heterologous Production ◽  
E Coli ◽  
Anticancer Properties ◽  
Defined Media ◽  
Extender Unit

Polyketides are a remarkable class of natural products with diverse functional and structural diversity. The class includes many medicinally important molecules with antiviral, antimicrobial, antifungal and anticancer properties. Native bacterial, fungal and plant hosts are often difficult to cultivate and coax into producing the desired product. As a result, Escherichia coli has been used for the heterologous production of polyketides, with the production of 6-deoxyerythronolide B (6-dEB) being the first example. Current strategies for production in E. coli require feeding of exogenous propionate as a source for the precursors propionyl-CoA and S-methylmalonyl-CoA. Here, we show that heterologous polyketide production is possible from glucose as the sole carbon source. The heterologous expression of eight genes from the Wood-Werkman cycle found in Propionibacteria, in combination with expression of the 6-dEB synthases DEBS1, DEBS2 and DEBS3 resulted in 6-dEB formation from glucose as the sole carbon source. Our results show that the Wood-Werkman cycle provides the required propionyl-CoA and the extender unit S-methylmalonyl-CoA to produce up to 0.81 mg/L of 6-dEB in a chemically defined media.

scholarly journals Enhanced Bacterial Growth and Gene Expression of D-Amino Acid Dehydrogenase With D-Glutamate as the Sole Carbon Source

2018 ◽  
Vol 9 ◽  
Author(s):  
Takeshi Naganuma ◽  
Yoshiakira Iinuma ◽  
Hitomi Nishiwaki ◽  
Ryota Murase ◽  
Kazuo Masaki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acid ◽  
Carbon Source ◽  
Bacterial Growth ◽  
Sole Carbon Source ◽  
Acid Dehydrogenase ◽  
Amino Acid Dehydrogenase

scholarly journals Carriage of Shiga toxin phage profoundly affects Escherichia coli gene expression and carbon source utilization

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Petya Berger ◽  
Ivan U. Kouzel ◽  
Michael Berger ◽  
Nadja Haarmann ◽  
Ulrich Dobrindt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Carbon Source ◽  
Shiga Toxin ◽  
Carbon Source Utilization
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