Syngas (mixture of CO, H2 and CO2) fermentation suffers from mass transfer limitation due to low solubility of CO and H2 in the liquid medium. Therefore, it is critical to characterize the mass transfer in syngas fermentation reactors to guide in delivery of syngas to the microorganisms. The objective of this study is to measure and predict the overall volumetric mass transfer coefficient, kLa for O2 at various operating conditions in a 7-L sparged and non-sparged continuous stirred-tank reactor (CSTR). Measurements indicated that the kLa for O2 increased with an increase in air flow rate and agitation speed. However, kLa for O2 decreased with the increase in the headspace pressure. The highest kLa for O2 with air sparged in the CSTR was 116 h−1 at 600 sccm, 900 rpm, 101 kPa, and 3 L working volume. Backmixing of the headspace N2 in the sparged CSTR reduced the observed kLa. The mass transfer model predicted the kLa for O2 within 10% of the experimental values. The model was extended to predict the kLa for syngas components CO, CO2 and H2, which will guide in selecting operating conditions that minimize power input to the bioreactor and maximize the syngas conversion efficiency.
Carbon monoxide and hydrogen mass transfer in a stirred tank reactor
