Cobalt(III)-promoted hydrolysis of glycine ethyl ester. Hg2+-induced acid hydrolysis and base hydrolysis of the .beta.2-Co(trien)Cl(Gly-oet)2+ ion

1970 ◽  
Vol 9 (1) ◽  
pp. 11-18 ◽  
Author(s):  
David A. Buckingham ◽  
D. M. Foster ◽  
L. G. Marzilli ◽  
Allan M. Sargeson

Nature ◽  
1965 ◽  
Vol 208 (5007) ◽  
pp. 265-267 ◽  
Author(s):  
MARGARET ROBSON




1962 ◽  
Vol 41 (2) ◽  
pp. 234-246 ◽  
Author(s):  
H. J. van der Molen

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.



Author(s):  
M. Alekseenko ◽  
V. Litvyak ◽  
A. Sysa ◽  
E. Hrabovska ◽  
O. Galenko


2012 ◽  
Vol 11 (12) ◽  
pp. 2313-2318
Author(s):  
Theodor Malutan ◽  
Adina Elena Panzariu


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