Rhodamine B and rhodamine 101 as reference substances for fluorescence quantum yield measurements

1980 ◽  
Vol 84 (14) ◽  
pp. 1871-1872 ◽  
Author(s):  
T. Karstens ◽  
K. Kobs
Keyword(s):  
Quantum Yield ◽  
Rhodamine B ◽  
Fluorescence Quantum Yield ◽  
Rhodamine 101

Fluorescent surfactants from common dyes – Rhodamine B and Eosin Y

2020 ◽  
Vol 92 (2) ◽  
pp. 265-274 ◽  
Author(s):  
Ashleigh D. Smith McWilliams ◽  
Selin Ergülen ◽  
Meredith M. Ogle ◽  
Carlos A. de los Reyes ◽  
Matteo Pasquali ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Chain Length ◽  
Rhodamine B ◽  
Fluorescence Quantum Yield ◽  
Fluorescent Dyes ◽  
Boron Nitride Nanotubes ◽  
Aliphatic Chain ◽  
Eosin Y ◽  
Imaging Tool ◽  
Potential Applications

AbstractEight fluorescent surfactants were synthesized by attaching aliphatic chains of 6, 10, 12, or 16 carbons to the fluorescent dyes Rhodamine B and Eosin Y. The obtained critical micelle concentrations (CMC) demonstrate an increasing CMC with decreasing aliphatic chain length, which is a typical behavior for surfactants. Additionally, fluorescence quantum yield experiments show a decrease in quantum yield with increasing aliphatic chain length, suggesting that the tails can interact with the dye, influencing its excited state. Finally, applications for the fluorescent surfactants were demonstrated; as a cellular stain in Panc-1 cells and as a dispersion and imaging tool for carbon and boron nitride nanotubes. These surfactants could provide a useful tool for a wide array of potential applications, from textile dyes to fluorescence imaging.

Investigations of the Reversible Energy Transfer in Concentrated Dye Solutions

1987 ◽  
Vol 42 (12) ◽  
pp. 1451-1455 ◽  
Author(s):  
C. Bojarski ◽  
G. Żurkowska
Keyword(s):  
Energy Transfer ◽  
Theoretical Model ◽  
Quantum Yield ◽  
Rhodamine B ◽  
Fluorescence Quantum Yield ◽  
Rhodamine 6G ◽  
Experimental Results ◽  
High Concentration ◽  
Concentration Region ◽  
Dye Solutions

In the system rhodamine 6G (donor)/rhodamine B (acceptor) the reverse energy transfer considerably increases the donor fluorescence quantum yield, particularly in the high concentration region. These experimental results confirm a theoretical model with no adjustable parameters.

Measurement of the absolute fluorescence quantum yield of rhodamine B solution using a dual-beam thermal lens technique

1996 ◽  
Vol 29 (4) ◽  
pp. 1074-1079 ◽  
Author(s):  
C V Bindhu ◽  
S S Harilal ◽  
Geetha K Varier ◽  
Riju C Issac ◽  
V P N Nampoori ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Rhodamine B ◽  
Thermal Lens ◽  
Fluorescence Quantum Yield ◽  
Dual Beam ◽  
The Absolute ◽  
Absolute Fluorescence

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

scholarly journals Synthesis of carbon nitride oligomer as a precursor of melon with improved fluorescence quantum yield

2021 ◽  
Vol 2 (18) ◽  
pp. 6083-6093
Author(s):  
Yuto Miyake ◽  
Goichiro Seo ◽  
Kotaro Matsuhashi ◽  
Noriyuki Takada ◽  
Kaname Kanai
Keyword(s):  
Optical Properties ◽  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Carbon Nitride

Melem tetramer, the newly synthesized carbon nitride compound is composed of oligomers with four melem units, is stable in air, and has improved optical properties compared to melon.

UV-Trained and Metal-Enhanced Fluorescence of Biliverdin and Biliverdin Nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Parinaz Fathi ◽  
Ayman Roslend ◽  
Kritika Mehta ◽  
Parikshit Moitra ◽  
Kai Zhang ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Biomedical Imaging ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence

Increasing the fluorescence quantum yield of fluorophores is of great interest for in vitro and in vivo biomedical imaging applications. At the same time, photobleaching and photodegradation resulting from continuous...

Internal Conversion with 3,5-Dimethyl-4-(methylamino)benzonitrile in Alkane Solvents

2000 ◽  
Vol 214 (11) ◽  
Author(s):  
I. Rückert ◽  
A. Hebecker ◽  
A.B.J. Parusel ◽  
K.A. Zachariasse
Keyword(s):  
Quantum Yield ◽  
Fluorescence Quantum Yield ◽  
Internal Conversion ◽  
Alkane Solvents

With 3,5-dimethyl-4-(methylamino)benzonitrile (MHD) in n-hexane and n-hexadecane at 25°C, relatively small values are obtained for the fluorescence quantum yield Φ

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