Oligonucleotide Synthesis:  Methods and Applications. Methods in Molecular Biology, Volume 288 Edited by Piet Herdewijn (Katholieke Universiteit Leuven, Belgium). Humana Press:  Totowa, NJ. 2005. xvi + 436 pp. $125.00. ISBN 1-58829-233-9.

2005 ◽  
Vol 127 (3) ◽  
pp. 1060-1060
Keyword(s):  
Molecular Biology ◽  
Synthesis Methods ◽  
Oligonucleotide Synthesis

Oligonucleotide synthesis interfaced with molecular biology and nanotechnology

2014 ◽  
Author(s):  
Marvin H. Caruthers ◽  
Subhadeep Roy ◽  
Heera Krishna ◽  
Ayman Alawneh ◽  
Shibasich Paul ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Oligonucleotide Synthesis

scholarly journals Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3′ Terminal Structures for Enzymatic De Novo DNA Synthesis

Genes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 102 ◽  
Author(s):  
Sebastian Barthel ◽  
Sebastian Palluk ◽  
Nathan J. Hillson ◽  
Jay D. Keasling ◽  
Daniel H. Arlow
Keyword(s):  
Elevated Temperatures ◽  
De Novo ◽  
Fold Increase ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Synthesis Methods ◽  
Transferase Activity ◽  
Oligonucleotide Synthesis ◽  
De Novo Synthesis ◽  
Aqueous Conditions ◽  
Terminal Structure

Enzymatic oligonucleotide synthesis methods based on the template-independent polymerase terminal deoxynucleotidyl transferase (TdT) promise to enable the de novo synthesis of long oligonucleotides under mild, aqueous conditions. Intermediates with a 3′ terminal structure (hairpins) will inevitably arise during synthesis, but TdT has poor activity on these structured substrates, limiting its usefulness for oligonucleotide synthesis. Here, we described two parallel efforts to improve the activity of TdT on hairpins: (1) optimization of the concentrations of the divalent cation cofactors and (2) engineering TdT for enhanced thermostability, enabling reactions at elevated temperatures. By combining both of these improvements, we obtained a ~10-fold increase in the elongation rate of a guanine-cytosine hairpin.

Electron Microscopy in Molecular Biology

1967 ◽  
Vol 25 ◽  
pp. 2-3
Author(s):  
Cecil E. Hall
Keyword(s):  
Electron Microscopy ◽  
Molecular Biology ◽  
Noise Level ◽  
Molecular Structures ◽  
Material Structure ◽  
The Arts ◽  
Shadow Casting ◽  
Electron Image ◽  
High Degree ◽  
Very High

The visualization of organic macromolecules such as proteins, nucleic acids, viruses and virus components has reached its high degree of effectiveness owing to refinements and reliability of instruments and to the invention of methods for enhancing the structure of these materials within the electron image. The latter techniques have been most important because what can be seen depends upon the molecular and atomic character of the object as modified which is rarely evident in the pristine material. Structure may thus be displayed by the arts of positive and negative staining, shadow casting, replication and other techniques. Enhancement of contrast, which delineates bounds of isolated macromolecules has been effected progressively over the years as illustrated in Figs. 1, 2, 3 and 4 by these methods. We now look to the future wondering what other visions are waiting to be seen. The instrument designers will need to exact from the arts of fabrication the performance that theory has prescribed as well as methods for phase and interference contrast with explorations of the potentialities of very high and very low voltages. Chemistry must play an increasingly important part in future progress by providing specific stain molecules of high visibility, substrates of vanishing “noise” level and means for preservation of molecular structures that usually exist in a solvated condition.

Molecular Biology and Biotechnology

2009 ◽  
Keyword(s):  
Molecular Biology

An introduction to the themed issue on RNA biology in China

2020 ◽  
Vol 64 (6) ◽  
pp. 863-866
Author(s):  
Zhe Wu
Keyword(s):  
Molecular Biology ◽  
Chinese Society ◽  
Fortieth Anniversary ◽  
Rna Biology ◽  
Research And Education ◽  
Education In China

Abstract The year 2019 marked the fortieth anniversary of the Chinese Society of Biochemistry and Molecular Biology (CSBMB), whose mission is to promote biomolecular research and education in China. The last 40 years have witnessed tremendous growth and achievements in biomolecular research by Chinese scientists and Essays in Biochemistry is delighted to publish this themed issue that focuses on exciting areas within RNA biology, with each review contributed by key experts from China.

Molecular Biology of Atrial Natriuretic Peptides

1987 ◽  
Vol 16 (1) ◽  
pp. 43-61 ◽  
Author(s):  
Hisayuki Matsuo ◽  
Hiroshi Nakazato
Keyword(s):  
Molecular Biology ◽  
Natriuretic Peptides ◽  
Atrial Natriuretic Peptides ◽  
Atrial Natriuretic
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